PBS is the everyday default buffer for most washing and dilution steps. Reach for HBSS or EBSS specifically when your protocol needs bicarbonate buffering matched to a CO2 incubator, or Ca\/Mg-free conditions for dissociation.<\/p>\n<\/div>\n
![\"PurMa](\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/PBS-1x-7.4_093024_BG.png\")
From PurMa Biologics<\/p>\n
The full buffer range<\/p>\n
PurMa manufactures HEPES 1M pH 8.0, TRIS 1M pH 8.0, EDTA 0.5M pH 8.0 and MES 0.5M pH 6.0, alongside Hanks’ and Earle’s balanced salt solutions at 1X pH 7.4.<\/p>\n
All buffers and salt solutions<\/a>HEPES Buffer 1M<\/a><\/p>\n Made in the USA and manufactured in house. Certificate of Analysis with every lot.<\/strong> Bulk pricing, purchase orders and custom formulations welcome.<\/p>\n<\/div>\n Cells are sensitive to their environment, and a good buffer protects them by:<\/p>\n Because these jobs differ, no single buffer is right for everything.<\/p>\n These contain inorganic salts (and often glucose) to maintain pH and osmotic balance while supplying ions.<\/p>\n HBSS (Hanks’ Balanced Salt Solution)<\/strong>\u00a0has low bicarbonate, so it holds a stable pH in normal room air \u2014 making it ideal for bench work: washing cells, prepping for dissociation, and short-term transport. Explore\u00a0HBSS<\/a>.<\/p>\n EBSS (Earle’s Balanced Salt Solution)<\/strong>\u00a0has high bicarbonate and is built for a 5% CO2 incubator, and it’s often used as a base for CO2-buffered media. Explore\u00a0EBSS<\/a>.<\/p>\n PBS (Phosphate Buffered Saline)<\/strong>\u00a0is a simple, phosphate-buffered isotonic solution<\/a> for washing cells and preparing dilutions \u2014 typically calcium- and magnesium-free. Explore\u00a0PBS<\/a>.<\/p>\n DPBS (Dulbecco’s Phosphate Buffered Saline)<\/strong>\u00a0is a PBS variant available with or without calcium and magnesium, used for cell washing and gentle handling. Explore\u00a0DPBS<\/a>.<\/p>\n When you need tighter pH control \u2014 especially\u00a0outside<\/em>\u00a0a CO2 incubator \u2014 zwitterionic buffers help.<\/p>\n HEPES<\/strong>\u00a0maintains a stable physiological pH (7.2\u20137.4) and is widely added to media for CO2-independent work and open-air procedures. Explore\u00a0HEPES<\/a>.<\/p>\n MES<\/strong>\u00a0buffers in a lower pH range (about 5.5\u20136.7), for applications that need a stable, slightly acidic pH. Explore\u00a0MES<\/a>.<\/p>\nWhat Do Buffers Do in Cell Culture?<\/h2>\n
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Balanced Salt Solutions (BSS)<\/h2>\n
pH Buffers<\/h3>\n
How to Choose the Right Cell Culture Buffer<\/h2>\n