{"id":100583,"date":"2024-07-05T10:43:54","date_gmt":"2024-07-05T16:43:54","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&p=100583"},"modified":"2026-08-13T13:47:14","modified_gmt":"2026-08-13T19:47:14","slug":"rpmi-1640-medium","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/rpmi-1640-medium\/","title":{"rendered":"PurMa\u2122 Roswell Park Memorial Institute 1640 (RPMI-1640)"},"content":{"rendered":"

PurMa\u2122 RPMI-1640 Cell Culture Medium \u2013 High\u2011Consistency Mammalian Cell Culture Media<\/strong><\/p>\n

Product Description<\/h2>\n

PurMa\u2122 RPMI 1640 medium is a high-performance cell culture formulation optimized for mammalian cells, including tumor and suspension lines. Originally developed in 1967 at Roswell Park Memorial Institute, RPMI-1640 is now a standard medium for suspension and tumor cells. Our optimized PurMa\u2122\u00a0 RPMI-1640 cell culture medium builds on this classic formulation to support robust proliferation and high experimental reproducibility. RPMI 1640 was originally developed to culture human cells.<\/p>\n

Not sure whether DMEM or RPMI 1640 is right for your cell line? See our comparison guide<\/a>.<\/p>\n

PurMa\u2122\u00a0 RPMI-1640 cell culture medium contains elevated phosphate levels that support tumor cells relying on glycolysis as their main energy source. This makes it especially suitable for cell aggregates and cancer research applications. The medium has a higher pH of approximately 7.6, and includes biotin, vitamin B12, and PABA, along with high concentrations of inositol and choline. These components help maintain healthy cell metabolism and stable long\u2011term cultures.<\/p>\n

We manufacture PurMa\u2122\u00a0 RPMI-1640 cell culture medium using 99% pure amino acids and strict quality controls. Our production is based on more than 30 years of experience in cutting\u2011edge cell and tissue culture science. PurMa Biologics routinely produces over 1500 media formulations, ensuring deep expertise in nutrient balance, sterility, and performance. This high level of purity and process control translates into superior consistency between batches and more comparable results between laboratories.<\/p>\n

PurMa\u2122\u00a0 RPMI-1640 cell culture medium is successfully used for culturing human normal and neoplastic leukocytes, HeLa, Jurkat, MCF\u20117, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells. Whether you work in oncology, immunology, neuroscience, or bioproduction, this medium provides a reliable platform for routine culture, assay development, and advanced research.<\/p>\n

Considering its quality and performance, PurMa\u2122\u00a0 RPMI-1640 cell culture medium is offered at an extremely reasonable price point. It is an excellent choice for laboratories that need consistent, high\u2011quality cell culture media without compromising their budget.<\/p>\n

FAQs<\/h2>\n

Q1. What is PurMa\u2122\u00a0 RPMI-1640 cell culture medium used for?<\/strong><\/h3>\n

PurMa RPMI-1640 cell culture medium is used for culturing human normal and neoplastic leukocytes, as well as many common cell lines. Typical applications include HeLa, Jurkat, MCF\u20117, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells. It is widely used in cancer research, immunology, and general mammalian cell biology.<\/p>\n

Q2. How is PurMa\u2122\u00a0 RPMI-1640 different from other cell culture media?<\/strong><\/h3>\n

PurMa RPMI-1640 cell culture medium has a higher pH of around 8 and contains elevated phosphate levels that support glycolysis\u2011dependent tumor cells. It also includes biotin, vitamin B12, and PABA, plus high concentrations of inositol and choline. These features distinguish it from other standard media and make it especially suitable for tumor and suspension cultures.<\/p>\n

Q3. Why should I choose PurMa RPMI-1640 over standard RPMI-1640?<\/strong>
\nPurMa\u2122\u00a0 RPMI-1640 cell culture medium is manufactured with 99% pure amino acids and strict quality controls. Our 30 years of experience and production of more than 1500 media formulations ensure excellent batch\u2011to\u2011batch consistency. This leads to more reliable data, closer alignment between experiments, and better comparability with results from other laboratories.<\/p>\n

Q4. Which cell types grow best in PurMa\u2122\u00a0 RPMI-1640 cell culture medium?<\/strong>
\nPurMa RPMI-1640 cell culture medium is ideal for leukocytes, hybridomas, and many tumor and suspension cell lines. It performs particularly well with HeLa, Jurkat, MCF\u20117, PC12, PBMC, astrocytes, carcinomas, and bone marrow cultures. Always confirm compatibility with your specific cell line and adapt supplements as needed.<\/p>\n

Q5. Does PurMa\u2122\u00a0 RPMI-1640 contain amino acids, vitamins, and other nutrients?<\/strong>
\nYes. PurMa RPMI-1640 cell culture medium contains a balanced mixture of amino acids, vitamins, and inorganic salts. In addition to its high phosphate level, it includes biotin, vitamin B12, PABA, inositol, and choline in high concentrations. These components help support stable growth and maintain healthy cell metabolism.<\/p>\n

Q6. Where can I find the full formulation of PurMa\u2122\u00a0 RPMI-1640 cell culture medium?<\/strong><\/h4>\n

The complete formulation of PurMa RPMI-1640 cell culture medium is available on our website. Please refer to the RPMI-1640 Formulation<\/em> section on this product page or follow the provided formulation link to see the full list of components and their concentrations.<\/p>\n

Formulation<\/h2>\n

Formulation:<\/strong> Complete formulation is here: RPMI-1640 Formulation<\/span><\/strong><\/a><\/p>\n

The Standard formulation of PurMa\u2122 RPMI-1640 is identical to Thermofisher, Gibco RPMI 1640 medium and slightly different than ATCC formulation. PurMa Biologics, however, manufactures the ATCC RPMI1640 routinely.<\/p>\n

References<\/h3>\n
    \n
  1. Culture of normal human leukocytes.<\/span> <\/a>Moore et al. 1967 Feb 20;199(8):519-24. PMID: 4960081<\/li>\n
  2. Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. <\/a><\/span>Dunne et al. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.<\/li>\n<\/ol>\n
    \n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    Parameter<\/th>\nSpecification<\/th>\n<\/tr>\n<\/thead>\n
    Appearance<\/td>\nRed, clear liquid<\/td>\n<\/tr>\n
    pH<\/td>\n\u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n
    Osmolality<\/td>\n\u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n
    Endotoxin<\/td>\n\u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n
    Mycoplasma<\/td>\nNegative<\/td>\n<\/tr>\n
    Suitability<\/td>\n\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n
    Additive<\/td>\n\u00a0Sodium pyruvate<\/a><\/td>\n<\/tr>\n
    Indicator<\/td>\n\u00a0Phenol red<\/td>\n<\/tr>\n
    Mycoplasma Detection<\/td>\nNegative<\/td>\n<\/tr>\n
    Sterility Tested<\/td>\n\u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n
    Form<\/td>\nLiquid<\/td>\n<\/tr>\n
    Shipping Condition<\/td>\n\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

    \n

    Learn more:<\/strong> What Is RPMI 1640?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    A high-performance RPMI-1640 optimized for mammalian cells, including tumor and suspension lines.<\/p>\n