{"id":100962,"date":"2024-07-06T21:31:42","date_gmt":"2024-07-07T03:31:42","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=100962"},"modified":"2026-07-24T15:22:41","modified_gmt":"2026-07-24T21:22:41","slug":"dmem-max-l-glutamine","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/dmem-max-l-glutamine\/","title":{"rendered":"PurMa\u2122 Dulbecco&#8217;s Modified Eagle&#8217;s Medium MAX (DMEM MAX)"},"content":{"rendered":"<p data-rm-block-id=\"block-1\"><strong>PurMa\u2122 Dulbecco\u2019s Modified Eagle\u2019s Medium MAX (DMEM MAX ) with L\u2011alanyl\u2011L\u2011glutamine\u00a0 \u2013 High Glucose, | PurMa Biologics<\/strong><\/p>\n<h2 data-rm-block-id=\"block-2\"><strong>Product Overview<\/strong><\/h2>\n<p data-rm-block-id=\"block-3\">PurMa Biologics<\/p>\n<p data-rm-block-id=\"block-4\">PurMa\u2122 Dulbecco\u2019s Modified Eagle\u2019s Medium MAX (DMEM MAX ) with L\u2011alanyl\u2011L\u2011glutamine is an advanced classical cell culture medium designed for <strong>mammalian cell culture<\/strong> that demands higher stability and performance than regular DMEM. DMEM\u2011MAX is optimized for <strong>primary cells and sensitive cell lines<\/strong>, providing superior buffering capacity and a more stable glutamine source.<\/p>\n<p data-rm-block-id=\"block-6\"><strong>Major difference vs. regular DMEM:<\/strong><br \/>\nStandard Dulbecco\u2019s Modified Eagle\u2019s Medium (DMEM) uses <strong>L\u2011glutamine<\/strong> supplementation. <strong>DMEM\u2011MAX replaces L\u2011glutamine with 4 mM L\u2011alanyl\u2011L\u2011glutamine<\/strong>, which greatly enhances stability and reduces toxic by\u2011products.<\/p>\n<h2 data-rm-block-id=\"block-8\"><strong>\u00a0Advantages of Dulbecco\u2019s Modified Eagle\u2019s Medium MAX (DMEM MAX ) with L\u2011alanyl\u2011L\u2011glutamine\u00a0<\/strong><\/h2>\n<p data-rm-block-id=\"block-9\">Compared with regular DMEM, PurMa Biologics Dulbecco\u2019s Modified Eagle\u2019s Medium MAX (DMEM MAX ) with L\u2011alanyl\u2011L\u2011glutamine offers:<\/p>\n<ul>\n<li data-rm-block-id=\"block-10\"><strong>Higher pH capacity<\/strong> for more stable culture conditions<\/li>\n<li data-rm-block-id=\"block-11\"><strong>4 mM L\u2011alanyl\u2011L\u2011glutamine (0.869 g\/L) instead of L\u2011glutamine<\/strong><\/li>\n<li data-rm-block-id=\"block-12\"><strong>Longer shelf life<\/strong> of the medium due to improved glutamine stability<\/li>\n<li data-rm-block-id=\"block-13\"><strong>Minimized toxic ammonia build\u2011up<\/strong> from glutamine degradation<\/li>\n<li data-rm-block-id=\"block-14\"><strong>Improved cell viability and growth<\/strong> in primary and continuous cell lines<\/li>\n<li data-rm-block-id=\"block-15\"><strong>Stability across a wide range of temperatures<\/strong><\/li>\n<li data-rm-block-id=\"block-16\"><strong>Enhanced performance for primary cell culture<\/strong>, including sensitive mammalian cells<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-17\">DMEM MAX with L\u2011Alanyl\u2011L\u2011Glutamine is ideal when you need <strong>consistent, high\u2011quality performance<\/strong> in demanding cell culture workflows.<\/p>\n<h2 data-rm-block-id=\"block-18\"><strong>Why L\u2011Alanyl\u2011L\u2011Glutamine Instead of L\u2011Glutamine? (Mechanism)<\/strong><\/h2>\n<p data-rm-block-id=\"block-19\">While <strong>L\u2011glutamine<\/strong> is an essential amino acid for mammalian cells, it is chemically unstable in aqueous solution. Over time, L\u2011glutamine degrades and generates <strong>toxic ammonia<\/strong> and <strong>pyrrolidone carboxylic acid<\/strong>, which can:<\/p>\n<ul>\n<li data-rm-block-id=\"block-20\">Stress or damage cells which is proven to be far less in Dulbecco&#8217;s Modified Eagle&#8217;s Medium Max<\/li>\n<li data-rm-block-id=\"block-21\">Reduce viability and growth<\/li>\n<li data-rm-block-id=\"block-22\">Compromise experimental reproducibility<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-23\">One way to address this is to frequently add fresh L\u2011glutamine to the medium, but this is labor\u2011intensive and difficult to control precisely.<\/p>\n<p data-rm-block-id=\"block-24\"><strong>PurMa\u2122 Dulbecco\u2019s Modified Eagle\u2019s Medium MAX (DMEM MAX ) with L\u2011alanyl\u2011L\u2011glutamine solves this problem<\/strong> by using <strong>L\u2011alanyl\u2011L\u2011glutamine<\/strong>, a dipeptide form that is:<\/p>\n<ul>\n<li data-rm-block-id=\"block-25\"><strong>Much more stable in aqueous solution<\/strong><\/li>\n<li data-rm-block-id=\"block-26\"><strong>Resistant to rapid degradation<\/strong>, limiting ammonia formation<\/li>\n<li data-rm-block-id=\"block-27\"><strong>Slowly cleaved by aminopeptidases<\/strong>, gradually releasing L\u2011glutamine and L\u2011alanine<\/li>\n<li data-rm-block-id=\"block-28\">Providing a <strong>sustained source of glutamine<\/strong> for protein synthesis and metabolism (e.g., TCA cycle)<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-29\">The result is <strong>healthier cells, less toxicity, and more reproducible results<\/strong> over longer culture periods.<\/p>\n<p data-rm-block-id=\"block-30\">In addition to our DMEM\u2011MAX formulations, PurMa Biologics also manufactures <strong>PurMa\u2122 GluaSup (Cat# P3S210559)<\/strong>, a standalone <strong>L\u2011alanyl\u2011L\u2011glutamine supplement<\/strong> designed to prevent L\u2011glutamine degradation in other media.<\/p>\n<h2 data-rm-block-id=\"block-31\"><strong>\u00a0DMEM\u2011MAX Composition and Features<\/strong><\/h2>\n<p data-rm-block-id=\"block-32\">PurMa Biologics <strong>Dulbecco\u2019s Modified Eagle\u2019s Medium\u2011MAX (DMEM\u2011MAX)<\/strong> contains:<\/p>\n<ul>\n<li data-rm-block-id=\"block-33\"><strong>4 mM L\u2011alanyl\u2011L\u2011glutamine (0.869 g\/L)<\/strong> \u2013 stable glutamine source<\/li>\n<li data-rm-block-id=\"block-34\"><strong>5 mM sodium pyruvate<\/strong> \u2013 additional energy substrate<\/li>\n<li data-rm-block-id=\"block-35\"><strong>High glucose (4.5 g\/L)<\/strong> \u2013 supports metabolically active, fast\u2011growing cells<\/li>\n<li data-rm-block-id=\"block-36\"><strong>Non\u2011essential amino acids (NEAA)<\/strong> \u2013 reduce biosynthetic burden on cells<\/li>\n<li data-rm-block-id=\"block-37\"><strong>3.7 g\/L sodium bicarbonate<\/strong> \u2013 enhanced buffering capacity<\/li>\n<li data-rm-block-id=\"block-38\"><strong>Phenol red<\/strong> \u2013 visual pH indicator<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-39\">PurMa Biologics manufactures <strong>several DMEM\u2011MAX variants<\/strong> based on different <strong>sodium bicarbonate and HEPES<\/strong> levels to fine\u2011tune pH capacity and buffering for your specific incubator CO\u2082 conditions and experimental requirements.<\/p>\n<h2 data-rm-block-id=\"block-40\"><strong>Serum Supplementation<\/strong><\/h2>\n<p data-rm-block-id=\"block-41\">Like classical DMEM, <strong>DMEM\u2011MAX contains no proteins, lipids, or growth factors<\/strong>. Therefore, it <strong>requires supplementation<\/strong>, commonly with <strong>10% Fetal Bovine Serum (FBS)<\/strong> or a suitable serum\u2011free or defined supplement appropriate for your cell type.<\/p>\n<h2 data-rm-block-id=\"block-42\"><strong>Applications of DMEM\u2011MAX<\/strong><\/h2>\n<p data-rm-block-id=\"block-43\">PurMa\u2122 Dulbecco\u2019s Modified Eagle\u2019s Medium\u2011MAX (DMEM\u2011MAX) is recommended for:<\/p>\n<ul>\n<li data-rm-block-id=\"block-44\">Primary mammalian cell cultures<\/li>\n<li data-rm-block-id=\"block-45\">Sensitive or fastidious cell lines requiring stable culture conditions<\/li>\n<li data-rm-block-id=\"block-46\">Long\u2011term cultures where ammonia build\u2011up is a concern<\/li>\n<li data-rm-block-id=\"block-47\">High\u2011density cultures and scale\u2011up experiments<\/li>\n<li data-rm-block-id=\"block-48\">Applications where <strong>standard DMEM<\/strong> does not provide sufficient stability or performance,\u00a0 Modified Eagle&#8217;s Medium Max DMEM works far better and more efficiently.<\/li>\n<li data-rm-block-id=\"block-49\">Lastly, based on our decades of experience, PurMa\u2122 Dulbecco\u2019s Modified Eagle\u2019s Medium MAX DMEM MAX L\u2011alanyl\u2011L\u2011glutamine is proven to grow any cell line properly.<\/li>\n<\/ul>\n<h2 data-rm-block-id=\"block-50\"><strong>Formulation Details<\/strong><\/h2>\n<p data-rm-block-id=\"block-51\">For a complete list of amino acids, vitamins, salts, and other components, please refer to the full <strong>DMEM\u2011MAX formulation<\/strong>:<strong><span style=\"color: #0000ff;\"><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/DMEM-MAX-Formulation-P3M110105.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"color: #0000ff;\">DMEM MAX Formulation<\/span><\/a><\/span><\/strong><\/p>\n<h2 data-rm-block-id=\"block-52\"><strong>FAQ\u00a0<\/strong><\/h2>\n<p data-rm-block-id=\"block-53\"><strong>What is the main difference between DMEM\u2011MAX and regular DMEM?<\/strong><br \/>\nDMEM\u2011MAX replaces unstable L\u2011glutamine with <strong>4 mM L\u2011alanyl\u2011L\u2011glutamine<\/strong>, providing higher stability, reduced ammonia build\u2011up, and improved cell viability compared with standard DMEM.<\/p>\n<p data-rm-block-id=\"block-54\"><strong>Does PurMa\u2122 Dulbecco\u2019s Modified Eagle\u2019s Medium MAX (DMEM MAX ) with L\u2011Alanyl\u2011L\u2011Glutamine, contain FBS?<\/strong><br \/>\nNo. <strong>DMEM\u2011MAX does not contain FBS, proteins, lipids, or growth factors<\/strong> and should be supplemented, typically with <strong>10% Fetal Bovine Serum (FBS)<\/strong> or an appropriate serum\u2011free supplement.<\/p>\n<p data-rm-block-id=\"block-55\"><strong>Why is L\u2011alanyl\u2011L\u2011glutamine better for long\u2011term culture?<\/strong><br \/>\nL\u2011alanyl\u2011L\u2011Glutamine is <strong>more stable in aqueous solution<\/strong> and does not rapidly degrade to ammonia. It is slowly cleaved by aminopeptidases to release L\u2011glutamine and L\u2011alanine, supporting cell growth while minimizing toxic by\u2011products.<\/p>\n<p data-rm-block-id=\"block-56\"><strong>H3: What cell types are suitable for DMEM\u2011MAX?<\/strong><br \/>\nDulbecco\u2019s Modified Eagle\u2019s Medium MAX (DMEM MAX ) with L\u2011Alanyl\u2011L\u2011Glutamine, is suitable for a wide range of <strong>mammalian cells<\/strong>, especially <strong>primary cells and sensitive cell lines<\/strong> that benefit from stable glutamine and enhanced buffering capacity.<\/p>\n<h2 data-rm-block-id=\"block-57\">References:<\/h2>\n<ol>\n<li data-rm-block-id=\"block-58\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/11182021\/\" rel=\"nofollow noopener\" target=\"_blank\">A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. <\/a><\/span>Hu J, Bok D. Mol Vis. 2001 Feb 7;7:14-9. PMID: 11182021<\/li>\n<li data-rm-block-id=\"block-59\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/21743014\/\" rel=\"nofollow noopener\" target=\"_blank\">Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19. <\/a><\/span>Ahmado A, et al., Invest Ophthalmol Vis Sci. 2011 Sep 9;52(10):7148-59. doi: 10.1167\/iovs.10-6374.PMID: 21743014<span style=\"color: #ffffff;\"><br \/>\n<\/span><\/li>\n<li data-rm-block-id=\"block-60\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27040228\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Protective layer formation on magnesium in cell culture medium. <\/span><\/a>Wagener V, et al., Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:341-51. doi: 10.1016\/j.msec.2016.03.003. Epub 2016 Mar 3.PMID: 27040228<\/li>\n<\/ol>\n<div class=\"table-1\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\" data-rm-block-id=\"block-61\">Parameter<\/th>\n<th align=\"left\" data-rm-block-id=\"block-62\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-63\">Appearance<\/td>\n<td align=\"left\" data-rm-block-id=\"block-64\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-65\">pH<\/td>\n<td align=\"left\" data-rm-block-id=\"block-66\">7.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-67\">Osmolality<\/td>\n<td align=\"left\" data-rm-block-id=\"block-68\">275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-69\">Endotoxin<\/td>\n<td align=\"left\" data-rm-block-id=\"block-70\">NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-71\">Mycoplasma<\/td>\n<td align=\"left\" data-rm-block-id=\"block-72\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-73\">Suitability<\/td>\n<td align=\"left\" data-rm-block-id=\"block-74\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-75\">Additive<\/td>\n<td align=\"left\" data-rm-block-id=\"block-76\">Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-77\">Indicator<\/td>\n<td align=\"left\" data-rm-block-id=\"block-78\">Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-79\">Mycoplasma Detection<\/td>\n<td align=\"left\" data-rm-block-id=\"block-80\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-81\">Sterility Tested<\/td>\n<td align=\"left\" data-rm-block-id=\"block-82\">Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-83\">Form<\/td>\n<td align=\"left\" data-rm-block-id=\"block-84\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-85\">Shipping Condition<\/td>\n<td align=\"left\" data-rm-block-id=\"block-86\">\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p data-rm-block-id=\"block-87\">\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-dmem\/\">What Is DMEM?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Dulbecco\u2019s Modified Eagle\u2019s Medium MAX (DMEM MAX ) with L\u2011Alanyl\u2011L\u2011Glutamine, therefore supplying a longer shelf life.\u00a0 MAX DMEM with lAlanyllglutamine significantly improves cell viability and growth. We manufacture several types of DMEM-MAX based on your requirements.<\/p>\n","protected":false},"featured_media":127452,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,509],"product_tag":[581,593,682,666,556,595,545,546],"class_list":["post-100962","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-dulbeccos-modified-eagles-medium-dmem","product_tag-cell-culture","product_tag-dmem","product_tag-glucose","product_tag-l-alanyl-l-glutamine","product_tag-mammalian-cells","product_tag-max","product_tag-phenol-red","product_tag-without-phenol-red","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/100962","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=100962"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127452"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=100962"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=100962"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=100962"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=100962"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}