{"id":102933,"date":"2024-07-08T22:08:48","date_gmt":"2024-07-09T04:08:48","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=102933"},"modified":"2026-07-24T15:22:43","modified_gmt":"2026-07-24T21:22:43","slug":"iscoves-modified-dulbeccos-medium-max","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/iscoves-modified-dulbeccos-medium-max\/","title":{"rendered":"PurMa\u2122Iscove&#8217;s Modified Dulbecco&#8217;s Medium MAX (IMDM-MAX)"},"content":{"rendered":"<h2 data-rm-block-id=\"block-1\"><strong>PurMa\u2122 Iscove\u2019s Modified Dulbecco\u2019s Medium MAX (IMDM\u2011MAX)<\/strong><\/h2>\n<h2>Product Description<\/h2>\n<p data-rm-block-id=\"block-2\">PurMa\u2122 IMDM MAX cell culture medium is an advanced\u00a0 form of <a href=\"https:\/\/purmabiologics.com\/blog\/product\/iscoves-modified-dulbeccos-medium-imdm\/\">IMDM<\/a> cell culture medium designed for high\u2011performance mammalian cell culture. It is ideal for labs that need robust, reproducible growth conditions for hybridomas, lymphocytes, and other fastidious cell lines.<\/p>\n<p data-rm-block-id=\"block-3\">The defining feature of IMDM\u2011MAX is its use of <strong>L\u2011alanyl\u2011L\u2011glutamine<\/strong> instead of conventional <strong>L\u2011glutamine<\/strong>. This unique MAX formulation is proprietary to PurMa Biologics and is engineered to support modern cell culture workflows with improved stability and reliability.<\/p>\n<p data-rm-block-id=\"block-4\"><strong>Why choose IMDM\u2011MAX over Regular IMDM?<\/strong><\/p>\n<p data-rm-block-id=\"block-5\">While iscoves modified dulbeccos medium IMDM is widely used, it has key limitations for long or sensitive cultures. IMDM\u2011MAX offers several practical advantages:<\/p>\n<ul>\n<li>\n<p data-rm-block-id=\"block-6\"><strong>L\u2011alanyl\u2011L\u2011glutamine instead of L\u2011glutamine<\/strong><br \/>\nL\u2011glutamine is chemically unstable in aqueous solution and degrades over time, producing ammonia that can stress cells. IMDM\u2011MAX replaces it with dipeptide L\u2011alanyl\u2011L\u2011glutamine, which is far more stable during storage and incubation.<\/p>\n<\/li>\n<li>\n<p data-rm-block-id=\"block-7\"><strong>Longer shelf life<\/strong><br \/>\nBecause L\u2011alanyl\u2011L\u2011glutamine is more stable, IMDM\u2011MAX maintains glutamine availability for a longer period. This helps preserve the functional quality of the medium over its entire shelf life, reducing waste and re\u2011preparation.<\/p>\n<\/li>\n<li>\n<p data-rm-block-id=\"block-8\"><strong>Higher buffering capacity<\/strong> IMDM\u2011MAX is formulated to better maintain pH under typical CO\u2082 incubator conditions. Stronger buffering helps keep your cultures in a more consistent environment, even with frequent door openings or higher metabolic activity.<\/p>\n<\/li>\n<\/ul>\n<ul>\n<li>\n<p data-rm-block-id=\"block-9\"><strong>Lower risk of contamination events<\/strong><br \/>\nMore stable nutrients and better pH control mean fewer stressful medium changes and less handling. Reduced handling often translates into fewer contamination opportunities and more consistent results across experiments.<\/p>\n<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-10\">Together, these differences make IMDM MAX cell culture medium a strong upgrade path for users of traditional IMDM iscoves modified dulbecco medium who need longer, cleaner, and more controlled cultures.<\/p>\n<p data-rm-block-id=\"block-11\"><strong>Key Features and Benefits<\/strong><\/p>\n<ul>\n<li>\n<p data-rm-block-id=\"block-12\"><strong>MAX formulation unique to PurMa Biologics<\/strong><br \/>\nOnly PurMa Biologics uses the MAX designation, which specifically indicates the use of L\u2011alanyl\u2011L\u2011glutamine in this IMDM MAX cell culture medium.<\/p>\n<\/li>\n<li>\n<p data-rm-block-id=\"block-13\"><strong>Optimized for mammalian cell culture<\/strong><br \/>\nSupports a wide range of mammalian cells, including suspension and adherent lines that demand a rich, balanced nutrient profile.<\/p>\n<\/li>\n<li>\n<p data-rm-block-id=\"block-14\"><strong>Improved stability during storage and use<\/strong><br \/>\nL\u2011alanyl\u2011L\u2011glutamine reduces glutamine breakdown and ammonia buildup, helping maintain a healthier culture environment over time.<\/p>\n<\/li>\n<li>\n<p data-rm-block-id=\"block-15\"><strong>Stronger pH control<\/strong><br \/>\nEnhanced buffering capacity supports stable pH across longer culture intervals, improving experimental consistency.<\/p>\n<\/li>\n<li>\n<p data-rm-block-id=\"block-16\"><strong>Lower contamination risk through fewer interventions<\/strong><br \/>\nMore stable medium can reduce the frequency of complete medium changes, decreasing the number of manipulations and potential contamination points.<\/p>\n<\/li>\n<li>\n<p data-rm-block-id=\"block-17\"><strong>Consistent, reproducible performance<\/strong><br \/>\nDesigned for labs that depend on stable cell performance across multiple passages, assays, and production runs.<\/p>\n<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-18\"><strong>Typical Applications<\/strong><\/p>\n<ul>\n<li data-rm-block-id=\"block-19\">Expansion and maintenance of mammalian cell lines<\/li>\n<li data-rm-block-id=\"block-20\">Hybridoma and antibody\u2011producing cell culture<\/li>\n<li data-rm-block-id=\"block-21\">Immunology, oncology, and vaccine research<\/li>\n<li data-rm-block-id=\"block-22\">Pre\u2011production and process development runs<\/li>\n<li data-rm-block-id=\"block-23\">Long\u2011term or higher\u2011density cultures where stability is critical<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-24\"><strong>How IMDM\u2011MAX Compares in Daily Lab Use<\/strong><\/p>\n<p data-rm-block-id=\"block-25\">Researchers moving from standard IMDM often notice:<\/p>\n<ul>\n<li data-rm-block-id=\"block-26\">More consistent growth curves over extended culture periods<\/li>\n<li data-rm-block-id=\"block-27\">Reduced variability between flasks and batches<\/li>\n<li data-rm-block-id=\"block-28\">Fewer culture failures linked to pH drift or glutamine degradation<\/li>\n<li data-rm-block-id=\"block-29\">Lower need for frequent medium replacement<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-30\">These practical gains can save time, reduce cost per usable culture, and improve confidence in downstream data and product quality.<\/p>\n<p data-rm-block-id=\"block-30\">PurMa Biologics manufactures 142 types of IMDM including IMDM with L-alanyl-L-glutamine without phenol red which is used a lot by laboratories.<\/p>\n<h3 data-rm-block-id=\"block-31\">Storage and Handling<\/h3>\n<ul>\n<li data-rm-block-id=\"block-32\">Store at the recommended temperature (typically 2\u20138 \u00b0C; check product label)<\/li>\n<li data-rm-block-id=\"block-33\">Protect from light where applicable<\/li>\n<li data-rm-block-id=\"block-34\">Use aseptic technique for all transfers and medium changes<\/li>\n<li data-rm-block-id=\"block-35\">Do not use if the medium shows turbidity, unexpected color change, or precipitates<\/li>\n<\/ul>\n<h4 data-rm-block-id=\"block-36\"><strong>FAQ<\/strong><\/h4>\n<p data-rm-block-id=\"block-37\"><strong>Q1. What is IMDM\u2011MAX and how is it different from standard IMDM?<\/strong><br \/>\nIMDM\u2011MAX is PurMa\u2122 Iscove\u2019s Modified Dulbecco\u2019s Medium formulated with L\u2011alanyl\u2011L\u2011glutamine instead of L\u2011glutamine. This MAX formulation is unique to PurMa Biologics and provides improved stability, longer shelf life, and better pH control compared to conventional IMDM preparations.<\/p>\n<p data-rm-block-id=\"block-38\"><strong>Q2. How does IMDM\u2011MAX differ from regular IMDM?<\/strong><br \/>\nThe major difference is the use of L\u2011alanyl\u2011L\u2011glutamine in IMDM MAX cell culture medium rather than L\u2011glutamine in regular IMDM. This makes the IMDM MAX cell culture medium more stable, with higher buffering capacity and a lower probability of contamination due to fewer required medium changes and interventions.<\/p>\n<p data-rm-block-id=\"block-39\"><strong>Q3. Why is L\u2011alanyl\u2011L\u2011glutamine beneficial in cell culture media?<\/strong><br \/>\nL\u2011alanyl\u2011L\u2011glutamine is a stable dipeptide that slowly releases glutamine over time. It resists degradation and ammonia formation better than free L\u2011glutamine, helping to maintain a healthier environment for mammalian cells during storage and incubation.<\/p>\n<p data-rm-block-id=\"block-40\"><strong>Q4. Which cell types are suitable for IMDM\u2011MAX?<\/strong><br \/>\nIMDM\u2011MAX is suitable for a broad range of mammalian cells, including suspension and adherent cell lines. It is especially useful for hybridomas, lymphocytes, and other demanding cells that benefit from a stable, nutrient\u2011rich IMDM MAX cell culture medium.<\/p>\n<p data-rm-block-id=\"block-41\"><strong>Q5. Does IMDM\u2011MAX reduce contamination in my cultures?<\/strong><br \/>\nIMDM MAX cell culture medium cannot eliminate contamination, but its improved stability and buffering reduce the need for frequent medium changes and handling. Less handling typically means fewer opportunities for contaminants to enter your cultures.<\/p>\n<p data-rm-block-id=\"block-42\"><strong>Q6. Can I switch from regular IMDM to IMDM\u2011MAX directly?<\/strong><br \/>\nMany labs can transition from Regular IMDM to IMDM\u2011MAX with a brief adaptation phase. It is good practice to test the IMDM MAX cell culture medium in parallel with your current medium and monitor growth, viability, and product expression before fully switching.<\/p>\n<p data-rm-block-id=\"block-43\"><strong>Q7. How should I store IMDM\u2011MAX?<\/strong><br \/>\nStore IMDM\u2011MAX at the temperature specified on the product label, typically 2\u20138 \u00b0C, and protect it from light if indicated. Always use sterile technique, and discard the bottle if you notice turbidity, unexpected color changes, or precipitates.<\/p>\n<p data-rm-block-id=\"block-44\"><strong>Q8. Is IMDM\u2011MAX supplied with serum or supplements?<\/strong><br \/>\nCheck the specific catalog number for details. IMDM\u2011MAX is available as a basal IMDM MAX cell culture medium that can be combined with your preferred serum and supplements, allowing you to match your existing protocols.<\/p>\n<h5 data-rm-block-id=\"block-45\">Formulation<\/h5>\n<p data-rm-block-id=\"block-46\">Complete formulation click here: <a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/02\/IMDM-MAX-Formulation-P3M110107.pdf\" target=\"_blank\" rel=\"noopener\"><strong><span style=\"color: #0000ff;\">IMDM-MAX Formulation\u00a0<\/span><\/strong><\/a><\/p>\n<p data-rm-block-id=\"block-47\"><strong>References<\/strong><\/p>\n<ul>\n<li data-rm-block-id=\"block-48\"><span style=\"color: #0000ff;\">Plaque production by the polyoma virus. <\/span>DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016\/0042-6822(59)90043-1. PMID: 13669362.<\/li>\n<li data-rm-block-id=\"block-49\"><span style=\"color: #0000ff;\">Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes<\/span>. J Exp Med. 1978 Mar 1; 147(3): 923\u2013933. doi:\u00a010.1084\/jem.147.3.923 PMCID: PMC2184195<\/li>\n<li data-rm-block-id=\"block-50\"><span style=\"color: #0000ff;\">Development of an infusible-grade solution for non-cryopreserved hematopoietic cell storage. <\/span>Burger SR, Hubel A, McCullough J. 1999;1(2):123-33. doi:10.1080\/0032472031000141250. PMID: 19746589<\/li>\n<\/ul>\n<div class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\" data-rm-block-id=\"block-51\">Parameter<\/th>\n<th align=\"left\" data-rm-block-id=\"block-52\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-53\">Appearance<\/td>\n<td align=\"left\" data-rm-block-id=\"block-54\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-55\">pH<\/td>\n<td align=\"left\" data-rm-block-id=\"block-56\">\u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-57\">Osmolality<\/td>\n<td align=\"left\" data-rm-block-id=\"block-58\">\u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-59\">Endotoxin<\/td>\n<td align=\"left\" data-rm-block-id=\"block-60\">\u00a0NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-61\">Mycoplasma<\/td>\n<td align=\"left\" data-rm-block-id=\"block-62\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-63\">Suitability<\/td>\n<td align=\"left\" data-rm-block-id=\"block-64\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-65\">Additive<\/td>\n<td align=\"left\" data-rm-block-id=\"block-66\">\u00a0Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-67\">Indicator<\/td>\n<td align=\"left\" data-rm-block-id=\"block-68\">\u00a0Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-69\">Mycoplasma Detection<\/td>\n<td align=\"left\" data-rm-block-id=\"block-70\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-71\">Sterility Tested<\/td>\n<td align=\"left\" data-rm-block-id=\"block-72\">\u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-73\">Form<\/td>\n<td align=\"left\" data-rm-block-id=\"block-74\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-75\">Shipping Condition<\/td>\n<td align=\"left\" data-rm-block-id=\"block-76\">\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p data-rm-block-id=\"block-77\">\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-imdm\/\">What Is IMDM?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>PurMa\u2122 Iscove\u2019s Modified Dulbecco\u2019s Medium MAX (IMDM\u2011MAX) is a premium IMDM with L alanyl Lglutamine\u00a0 cell culture medium optimized for demanding mammalian cells. Unlike standard IMDM, IMDM\u2011MAX uses stable L\u2011alanyl\u2011L\u2011glutamine instead of L\u2011glutamine. This provides a longer shelf life, stronger buffering capacity, and reduced risk of contamination, helping you achieve more consistent, reliable cell culture performance.<\/p>\n","protected":false},"featured_media":127455,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,597],"product_tag":[581,682,666,556,595,545,546],"class_list":["post-102933","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-iscoves-modified-dulbeccos-medium-imdm","product_tag-cell-culture","product_tag-glucose","product_tag-l-alanyl-l-glutamine","product_tag-mammalian-cells","product_tag-max","product_tag-phenol-red","product_tag-without-phenol-red","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/102933","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=102933"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127455"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=102933"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=102933"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=102933"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=102933"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}