Eagle\u2019s Minimum Essential Medium, commonly called EMEM or MEM, is a foundational mammalian cell culture medium developed from Harry Eagle\u2019s earlier Basal Medium Eagle. It provides essential amino acids, vitamins, inorganic salts, and glucose to support cell maintenance and proliferation.<\/p>\n
Standard EMEM is commonly used for adherent mammalian cell lines, but the correct medium and supplements should always be selected according to the requirements of the specific cell line.<\/p>\n
PurMa\u2019s standard EMEM formulation contains:<\/p>\n
A sodium bicarbonate concentration of 1.5 g\/L is commonly used with a humidified 5% CO\u2082 atmosphere. Researchers should confirm the required bicarbonate level, supplements, and incubation conditions for their individual cell line.<\/p>\n
PurMa manufactures EMEM in standard and customized formulations, including options without L-glutamine, phenol red, sodium bicarbonate, sodium pyruvate, or glucose. High-glucose, high-bicarbonate, and HEPES-buffered formulations are also available.<\/p>\n
Choose the formulation, format, and package size above, or contact PurMa<\/a> for assistance matching EMEM to your protocol.<\/p>\n EMEM is commonly used as a basal medium for adherent mammalian cells in research areas such as virology, cancer biology, toxicology, and bioproduction. Because medium requirements vary among cell lines, use the formulation recommended by the cell-line supplier or a validated laboratory protocol.<\/p>\n For adapted suspension cultures, use a formulation specifically validated for suspension growth rather than assuming standard EMEM is interchangeable.<\/p>\n for complete formulation click here:\u00a0 EMEM Formulation<\/a>\u00a0<\/span><\/strong><\/p>\n References<\/p>\n \nApplications<\/h2>\n
Formulation<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0Sodium pyruvate<\/a><\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n