Eagle\u2019s Minimal Essential Medium-MAX (EMEM-MAX) is a commonly used media for Mammalian cell culture.<\/p>\n
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.<\/p>\n
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and is gradually cleaved by aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.<\/p>\n
In addition to our Max formulation products, we manufacture PurMaTM <\/sup>GluaSup (Cat# P3S210 559), a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.<\/p>\n Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)\u00a0contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).<\/p>\n The complete formulation for Eagle’s Minimal Essential Medium-MAX (EMEM-MAX) can be found here: EMEM-MAX Formulation<\/strong><\/span><\/a>\u00a0<\/span><\/p>\n <\/p>\nContents<\/h2>\n
Eagle\u2019s Minimal Essential Medium-MAX (EMEM-MAX) contains:<\/h3>\n
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Formulation<\/h2>\n
References<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n