{"id":104463,"date":"2024-07-10T15:58:04","date_gmt":"2024-07-10T21:58:04","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=104463"},"modified":"2026-07-24T15:22:44","modified_gmt":"2026-07-24T21:22:44","slug":"chemically-defined-f12k-medium","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/chemically-defined-f12k-medium\/","title":{"rendered":"PurMa\u2122 Chemically Defined Kaighn\u2019s Modification of Ham\u2019s F-12 Medium (CD-Ham\u2019s F-12K)"},"content":{"rendered":"<p><strong>PurMa\u2122 Chemically Defined Kaighn\u2019s Modification of Ham\u2019s F-12 Medium (CD-Ham\u2019s F-12K)<\/strong><\/p>\n<h2><strong>Product Description<\/strong><\/h2>\n<p>PurMa\u2122 Chemically Defined Kaighn&#8217;s Modification of Ham\u2019s F-12 Medium (CD-Ham\u2019s F-12K) is a <strong>chemically defined Ham\u2019s F-12K medium.<\/strong> It is\u00a0 specifically engineered for serum-free mammalian cell culture. Moreover, this formulation is <strong>100% synthetic<\/strong>, <strong>FBS-free<\/strong>, and free of undefined animal-derived components. Consequently, this provides a highly controlled environment for in vitro cell growth and advanced research applications. Unlike conventional media, which require ~10% fetal bovine serum (FBS), PurMa\u2122 CD-Ham\u2019s F-12K relies on <strong>known quantities of pure chemicals<\/strong> and a <strong>proprietary synthetic mixture of growth factors, hormones, and nutrients<\/strong>. More importantly, this eliminates lot-to-lot variability. Compared to using serum, it supports reproducible, scalable experiments that align with regulatory expectations.<\/p>\n<h3>Key Features &amp; Benefits<\/h3>\n<p><strong>Chemically defined Ham\u2019s F-12K medium<\/strong><br \/>\n\u2013 No FBS and no trace animal-derived components<br \/>\n\u2013 100% synthetic formulation for maximal control<\/p>\n<ul>\n<li><strong>Serum-free, animal-component\u2013free<\/strong><br \/>\n\u2013 Reduces biological variability and risk of contamination from serum<br \/>\n\u2013 Facilitates regulatory compliance and documentation<\/li>\n<li><strong>Enhanced stability with L\u2011alanyl\u2011L\u2011glutamine<\/strong><br \/>\n\u2013 Uses L\u2011alanyl\u2011L\u2011glutamine instead of unstable L\u2011glutamine<br \/>\n\u2013 Improved shelf life and reduced degradation by-products<br \/>\n\u2013 Higher buffering capacity, contributing to a more stable pH and lower contamination risk<\/li>\n<li><strong>Consistent performance<\/strong><br \/>\n\u2013 Defined composition of amino acids, vitamins, salts, sugars, and synthetic growth factors<br \/>\n\u2013 Supports reproducible cell growth and functional assays<\/li>\n<li><strong>Flexible for multiple cell types<\/strong><br \/>\n\u2013 Evidently proven to be suitable for customization of primary\u00a0 rat and chicken long term culture.<br \/>\n\u2013 Can be adapted to specific mammalian cell lines via supplements or user-defined additives<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-124479 aligncenter\" src=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Ham-F12-Graph-picture-1080x649.png\" alt=\"Ham F12 Graph \" width=\"566\" height=\"340\" srcset=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Ham-F12-Graph-picture-1080x649.png 1080w, https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Ham-F12-Graph-picture-768x461.png 768w, https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Ham-F12-Graph-picture-1536x923.png 1536w, https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Ham-F12-Graph-picture-500x300.png 500w, https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Ham-F12-Graph-picture-700x421.png 700w, https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Ham-F12-Graph-picture.png 1653w\" sizes=\"(max-width: 566px) 100vw, 566px\" \/><\/p>\n<p><strong>Applications<\/strong><\/p>\n<p><strong>PurMa\u2122 CD F12K Kaighn&#8217;s medium is ideal for:<\/strong><\/p>\n<ul>\n<li>Serum-free and animal-component\u2013free mammalian cell culture<\/li>\n<li>Studies where experimental reproducibility is critical<\/li>\n<li>Regulatory-sensitive workflows (bioprocess development, preclinical studies, QC labs)<\/li>\n<li>Mechanistic studies of cellular signaling without confounding serum components<\/li>\n<li>Protocols requiring precise control over media composition<\/li>\n<\/ul>\n<p><strong>Gradual Adaptation from FBS-Containing Media<\/strong><\/p>\n<p>For optimal results, gradually adapt cells from conventional FBS-containing media to PurMa\u2122 <strong>chemically defined Ham\u2019s F-12K medium<\/strong>. A stepwise reduction of FBS supports cell survival and stable growth.<\/p>\n<p><strong>Recommended adaptation strategy<\/strong><\/p>\n<ol>\n<li><strong>Set up controls<\/strong>\n<ul>\n<li>Control 1: Standard growth medium + 10% FBS (no chemically defined medium)<\/li>\n<li>Control 2: PurMa\u2122 CD-Ham\u2019s F-12K + 10% FBS (to monitor adaptation vs. standard)<\/li>\n<li>Additionally, we recommend adding <a href=\"https:\/\/purmabiologics.com\/blog\/product\/high-fungicide-purma-antibiotic-mix-solution\/\"><span style=\"color: #0000ff;\">PurMa\u2122 Antibiotic Mix<\/span><\/a> for preventing contamination.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Step wise FBS reduction<\/strong><br \/>\nEvery 48 hours, fully replace the medium as follows: spin down cells each time, discard the spent medium completely, and resuspend in fresh medium:<\/p>\n<ul>\n<li>Step 1: PurMa\u2122 CD-Ham\u2019s F-12K + 10% FBS<\/li>\n<li>Step 2 (48 h): PurMa\u2122 CD-Ham\u2019s F-12K + 8% FBS<\/li>\n<li>Step 3 (48 h): PurMa\u2122 CD-Ham\u2019s F-12K + 6% FBS<\/li>\n<li>Step 4 (48 h): PurMa\u2122 CD-Ham\u2019s F-12K + 4% FBS<\/li>\n<li>Step 5 (48 h): PurMa\u2122 CD-Ham\u2019s F-12K + 2% FBS<\/li>\n<li>Step 6 (48 h): PurMa\u2122 CD-Ham\u2019s F-12K + 1% FBS<\/li>\n<li>Final step (24 h later): <strong>PurMa\u2122 CD-Ham\u2019s F-12K with 0% FBS<\/strong> (fully serum-free)<\/li>\n<\/ul>\n<\/li>\n<li><strong>Practical notes<\/strong>\n<ul>\n<li>You do not need an extremely high starting density; ~500,000 cells\/mL is not mandatory.<\/li>\n<li>Use multiple flasks\/plates in parallel, as some cultures may fail or become contaminated during adaptation.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Post-adaptation handling<\/strong>\n<ul>\n<li>Once cells are fully adapted, maintain them exclusively in the same <strong>chemically defined Ham\u2019s F-12K medium<\/strong> from the same vendor and formulation, including for freezing and recovery.<\/li>\n<li>After adaptation to PurMa\u2122 CD-Ham\u2019s F-12K, expand the culture, prepare multiple plates, and freeze several vials for backup.<\/li>\n<li>For the first week after thawing adapted cells, supplement PurMa\u2122 CD-Ham\u2019s F-12K with <strong>10% PurMa\u2122 CD Booster (Cat# P3C10144)<\/strong> to support robust recovery.<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<p><strong>Formulation Highlights<\/strong><\/p>\n<p>The base formulation of PurMa\u2122 CD-Ham\u2019s F-12K (Cat# P3C210108) includes:<\/p>\n<ul>\n<li>Balanced inorganic salts for osmolarity and ionic strength<\/li>\n<li>Carbohydrate energy sources (e.g., sugars)<\/li>\n<li>Defined amino acids, including <strong>L\u2011alanyl\u2011L\u2011glutamine<\/strong> as the stable glutamine source<\/li>\n<li>Vitamins and trace elements<\/li>\n<li>A proprietary, fully synthetic cocktail of growth factors, hormones, and other nutrients<\/li>\n<\/ul>\n<p>This composition allows PurMa\u2122 CD-Ham\u2019s F-12K to function as a <strong>standalone, chemically defined Ham\u2019s F-12K medium<\/strong> without serum supplementation, while maintaining robust cell growth and viability.<\/p>\n<h4><strong>Storage &amp; Handling<\/strong><\/h4>\n<ul>\n<li>Store according to product label (typically 2\u20138 \u00b0C, protected from light)<\/li>\n<li>Avoid repeated freeze\u2013thaw cycles if applicable<\/li>\n<li>Use aseptic technique at all times to minimize the risk of contamination<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Formulation<\/strong><\/p>\n<p>The CD-Ham\u2019s F-12K includes\u00a0 L-Alanine -L glutamine :<a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Base-formulation-of-CD-Hams-F-12K-P3C210108-1.pdf\"><span style=\"color: #0000ff;\"><strong>Base formulation of CD Ham&#8217;s F-12K P3C210108<\/strong><\/span><\/a><\/p>\n<p><strong>Additionally, CD-Ham\u2019s F-12K contains a proprietary synthetic cocktail of specific growth factors, hormones, and other\u00a0 nutrients which makes it independent of any serum and animal products. <\/strong>Moreover, for more details about the base media, read\u00a0 <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/purmabiologics.com\/blog\/product\/hams-f-12k-l-alanyl-l-glutamineanyll-l-glutamine-hams-f-12k-l-alanyl-l-glutamine\/\">HamF12-MAX\u00a0<\/a><\/span><\/p>\n<p><strong>References<\/strong><\/p>\n<ul>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/6792277\/\" rel=\"nofollow noopener\" target=\"_blank\">Primary in vitro antibody responses by purified murine B lymphocytes in serum-free defined medium. <\/a><\/span>Mosier DE. J Immunol. 1981 Oct;127(4):1490-3. PMID: 6792277<\/li>\n<\/ul>\n<div class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\">Parameter<\/th>\n<th align=\"left\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\">Appearance<\/td>\n<td align=\"left\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">pH<\/td>\n<td align=\"left\">\u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Osmolality<\/td>\n<td align=\"left\">\u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Endotoxin<\/td>\n<td align=\"left\">\u00a0NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Mycoplasma<\/td>\n<td align=\"left\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Suitability<\/td>\n<td align=\"left\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Additive<\/td>\n<td align=\"left\">\u00a0Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Indicator<\/td>\n<td align=\"left\">\u00a0Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Mycoplasma Detection<\/td>\n<td align=\"left\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Sterility Tested<\/td>\n<td align=\"left\">\u00a0Sterile filtered using 0.1 \u00b5m filter, 3 Times<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Form<\/td>\n<td align=\"left\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Shipping Condition<\/td>\n<td align=\"left\">Room temperature, Overnight<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>&nbsp;<\/p>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-hams-f-12k\/\">What Is Ham&#8217;s F-12K?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>PurMa\u2122 Chemically Defined Kaighn\u2019s Modification of Hams F12 Medium (CD-Ham\u2019s F12K) is a 100% synthetic<strong>. It is FBS-free, animal-component\u2013free, chemically defined Ham\u2019s F-12K medium<\/strong>. Additionally, it replaces serum with a proprietary synthetic cocktail of growth factors, hormones, and nutrients. This ensures batch-to-batch consistency and regulatory-friendly workflows. Moreover, CD-Ham\u2019s F-12K contains <strong>L\u2011alanyl\u2011L\u2011glutamine<\/strong> instead of L\u2011glutamine. This provides improved stability, longer shelf life, and enhanced buffering capacity to support robust, reproducible mammalian cell culture.<\/p>\n","protected":false},"featured_media":124361,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,511],"product_tag":[596,581,670,529,668,530,666,669,667],"class_list":["post-104463","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-kaighns-modification-of-hams-f-12-medium-hams-f-12k","product_tag-advanced","product_tag-cell-culture","product_tag-f-12k","product_tag-hams-f-12k","product_tag-human-transferrin","product_tag-kaighns","product_tag-l-alanyl-l-glutamine","product_tag-recombinant-insulin","product_tag-serum-reduction","entry","has-media","first","instock","taxable","shipping-taxable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/104463","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=104463"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/124361"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=104463"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=104463"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=104463"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=104463"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}