{"id":104671,"date":"2024-07-10T16:46:59","date_gmt":"2024-07-10T22:46:59","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=104671"},"modified":"2026-07-24T15:22:42","modified_gmt":"2026-07-24T21:22:42","slug":"chemically-defined-rpmi-1640-medium","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/chemically-defined-rpmi-1640-medium\/","title":{"rendered":"PurMa\u2122Chemically Defined Roswell-Park Memorial Institute 1640 (CD-RPMI-1640)"},"content":{"rendered":"<h2 data-rm-block-id=\"block-1\">Product Description<\/h2>\n<p data-rm-block-id=\"block-2\">PurMa\u2122 Chemically Defined Roswell-Park Memorial Institute 1640 (CD-RPMI-1640) is a fully synthetic, serum-free medium optimized for mammalian cell culture. Unlike conventional RPMI that requires 10% FBS, <strong>PurMa chemically defined RPMI 1640<\/strong> contains no serum, no FBS, and no traces of animal-derived components. Every ingredient is known, controlled, and chemically defined.<\/p>\n<p data-rm-block-id=\"block-3\">This medium is ideal when you need reproducible performance, regulatory compliance, and clean experimental backgrounds without undefined serum variables.<\/p>\n<h2 data-rm-block-id=\"block-4\"><\/h2>\n<h3 data-rm-block-id=\"block-5\"><\/h3>\n<h3 data-rm-block-id=\"block-6\"><\/h3>\n<h3 data-rm-block-id=\"block-7\">Key Features and Benefits<\/h3>\n<ul>\n<li data-rm-block-id=\"block-8\"><strong>100% chemically defined, FBS-free formulation<\/strong><br \/>\nCD RPMI 1640 medium contains no serum, no FBS, and no animal-origin components. This reduces variability, simplifies regulatory documentation, and supports serum-independent workflows.<\/li>\n<li data-rm-block-id=\"block-9\"><strong>L\u2011alanyl\u2011L\u2011glutamine instead of L\u2011glutamine<\/strong><br \/>\nThe base of <strong>PurMa chemically defined RPMI 1640<\/strong> uses L\u2011alanyl\u2011L\u2011glutamine for improved stability. This dipeptide minimizes glutamine breakdown, extends shelf life, and enhances buffering capacity, which can lower the risk of contamination and pH drift.<\/li>\n<li data-rm-block-id=\"block-10\"><strong>Proprietary synthetic growth factor cocktail<\/strong><br \/>\nA defined blend of synthetic growth factors, hormones, and nutrients replaces serum functions. The result is reliable growth and viability without serum supplementation or animal-derived additives.<\/li>\n<li data-rm-block-id=\"block-11\"><strong>Consistent, controlled environment<\/strong><br \/>\nEvery component in CD-RPMI-1640 is characterized and quantified. This allows precise interpretation of cellular responses, improved assay reproducibility, and easier method transfer across labs.<\/li>\n<li data-rm-block-id=\"block-12\"><strong>Optimized for a wide range of mammalian cells<\/strong><br \/>\nThe formulation supports adaptation of many cell lines typically maintained in classical RPMI + 10% FBS. Once adapted, cells can be maintained and frozen using the same <strong>PurMa chemically defined RPMI 1640<\/strong> system.<\/li>\n<li class=\"flex w-full max-w-full flex-col gap-3 text-base! min-w-0 text-sm whitespace-normal [&amp;_a]:text-core-foreground-tertiary [&amp;_a]:leading-tight [&amp;_a]:font-semibold [&amp;_a]:underline [&amp;_strong]:font-bold [&amp;_p]:m-0 [&amp;_p]:whitespace-pre-wrap [&amp;_ol]:my-5 [&amp;_ol]:list-decimal [&amp;_ol]:pl-5 [&amp;_ul]:mt-2 [&amp;_ul]:list-disc [&amp;_ul]:pl-5 [&amp;_li]:my-3 [&amp;_li]:list-item [&amp;_li:first-child]:mt-0 [&amp;_ul&gt;li_p]:m-0 [&amp;_li&gt;ol]:mt-3 [&amp;_li&gt;ul]:mt-3 [&amp;_hr]:border-core-borders-default [&amp;_hr]:my-3 [&amp;_hr]:border-t-[1.5px] [&amp;_hr]:border-r-0 [&amp;_hr]:border-b-0 [&amp;_hr]:border-l-0 [&amp;_blockquote]:border-core-borders-default [&amp;_blockquote]:text-core-foreground-secondary [&amp;_blockquote]:border-l-4 [&amp;_blockquote]:pl-4 [&amp;_blockquote]:italic [&amp;_blockquote_p:first-of-type]:before:content-[open-quote] [&amp;_blockquote_p:last-of-type]:after:content-[close-quote] [&amp;_h1]:mt-4 [&amp;_h1]:mb-3 [&amp;_h1]:text-4xl [&amp;_h1]:leading-tight [&amp;_h1]:font-bold [&amp;_h1]:tracking-wide [&amp;_h2]:mt-4 [&amp;_h2]:mb-3 [&amp;_h2]:text-3xl [&amp;_h2]:leading-tight [&amp;_h2]:font-semibold [&amp;_h2]:tracking-wide [&amp;_h3]:mt-4 [&amp;_h3]:mb-3 [&amp;_h3]:text-2xl [&amp;_h3]:leading-tight [&amp;_h3]:font-semibold [&amp;_h3]:tracking-wide [&amp;_h4]:mt-4 [&amp;_h4]:mb-3 [&amp;_h4]:font-semibold [&amp;_h5]:mt-4 [&amp;_h5]:mb-3 [&amp;_h5]:font-semibold [&amp;_img]:my-3 [&amp;_figure&gt;*]:my-0 [&amp;_video]:my-8 [&amp;_figure]:my-8 [&amp;_:not(pre)&gt;code]:before:content-[&quot;&#96;&quot;] [&amp;_:not(pre)&gt;code]:after:content-[&quot;&#96;&quot;] [&amp;_pre_code]:before:content-none [&amp;_pre_code]:after:content-none [&amp;_a_code]:text-inherit [&amp;_h1_code]:text-inherit [&amp;_h2_code]:text-inherit [&amp;_h3_code]:text-inherit [&amp;_h4_code]:text-inherit [&amp;_blockquote_code]:text-inherit [&amp;_thead_th_code]:text-inherit [&amp;_hr+*]:mt-0 [&amp;_h2+*]:mt-0 [&amp;_h3+*]:mt-0 [&amp;_h4+*]:mt-0 [&amp;_table]:w-full [&amp;_table]:table-auto [&amp;_table]:border-separate [&amp;_table]:border-spacing-0 [&amp;_table]:text-left [&amp;_table]:block [&amp;_table]:overflow-x-auto [&amp;_thead]:bg-core-surface-secondary [&amp;_thead_th]:border-core-borders-default [&amp;_thead_th]:border [&amp;_thead_th]:p-2.5 [&amp;_thead_th]:px-4 [&amp;_thead_th]:text-sm [&amp;_thead_th:first-child]:rounded-tl-lg [&amp;_thead_th:last-child]:rounded-tr-lg [&amp;_thead_th:not(:first-child)]:border-l-0 [&amp;_tbody_td]:border-core-borders-default [&amp;_tbody_td]:border [&amp;_tbody_td]:border-t-0 [&amp;_tbody_td]:p-2.5 [&amp;_tbody_td]:px-4 [&amp;_tbody_td:not(:first-child)]:border-l-0 [&amp;_tbody_tr:last-child_td:first-child]:rounded-bl-lg [&amp;_tbody_tr:last-child_td:last-child]:rounded-br-lg *:first:mt-0 *:last:mb-0\" data-rm-block-id=\"block-13\">Stemcell technologies RPMI 1640 is a widely used cell culture medium that supports reliable growth and maintenance of diverse cell lines for advanced research applications.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h2 data-rm-block-id=\"block-4\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-124478 aligncenter\" src=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/RPMI-Graph-Picture-1080x649.png\" alt=\"Chemically Defined RPMI Vs RPMI1640 with FBS\" width=\"596\" height=\"358\" srcset=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/RPMI-Graph-Picture-1080x649.png 1080w, https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/RPMI-Graph-Picture-768x461.png 768w, https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/RPMI-Graph-Picture-1536x923.png 1536w, https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/RPMI-Graph-Picture-500x300.png 500w, https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/RPMI-Graph-Picture-700x421.png 700w, https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/RPMI-Graph-Picture.png 1653w\" sizes=\"(max-width: 596px) 100vw, 596px\" \/><\/h2>\n<p>&nbsp;<\/p>\n<p><strong>Formulation<\/strong><\/p>\n<ul>\n<li data-rm-block-id=\"block-15\">The CD-RPMI-1640 formulation includes:<strong>\u00a0 \u00a0 <a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Chemically-Defined-RPMI-1640-Formulation-P3C310109-3.pdf\">Base formulation of Chemically Defined RPMI-1640 , P3C310109<\/a><\/strong><\/li>\n<li data-rm-block-id=\"block-16\">Base chemically defined RPMI-1640 with L\u2011alanyl\u2011L\u2011glutamine<br \/>\nChemically Defined RPMI-1640, L\u2011alanine\u2013L\u2011glutamine base (Cat# P3C310109).<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li data-rm-block-id=\"block-17\"><strong>Synthetic growth factor and hormone cocktail<\/strong><br \/>\nA proprietary mixture of fully synthetic growth factors, hormones, and nutrients supports proliferation and viability without any serum or animal-derived components.<\/li>\n<li data-rm-block-id=\"block-18\">This composition allows truly serum-independent culture while preserving performance for demanding research and preclinical workflows.<\/li>\n<li data-rm-block-id=\"block-19\">Gradual Transition from FBS-Containing Media<\/li>\n<li data-rm-block-id=\"block-20\">For reliable adaptation from 10% FBS media to <strong>PurMa chemically defined RPMI 1640<\/strong>, a stepwise reduction of FBS is strongly recommended. Use at least two control conditions:<\/li>\n<\/ul>\n<ol>\n<li data-rm-block-id=\"block-21\">A reference culture maintained in standard RPMI + 10% FBS only.<\/li>\n<li data-rm-block-id=\"block-22\">A culture in <strong>PurMa chemically defined RPMI 1640<\/strong> + 10% FBS that will undergo staged FBS reduction.<\/li>\n<\/ol>\n<ul>\n<li data-rm-block-id=\"block-23\">A typical transition schedule:<\/li>\n<li data-rm-block-id=\"block-24\">Start: 10% FBS + PurMa\u2122 Chemically Defined Medium (adaptation culture)<\/li>\n<li data-rm-block-id=\"block-25\">After 48 hours: 8% FBS + PurMa\u2122 Chemically Defined Medium<\/li>\n<li data-rm-block-id=\"block-26\">After 48 hours: 6% FBS + PurMa\u2122 Chemically Defined Medium<\/li>\n<li data-rm-block-id=\"block-27\">After 48 hours: 4% FBS + PurMa\u2122 Chemically Defined Medium<\/li>\n<li data-rm-block-id=\"block-28\">After 48 hours: 2% FBS + PurMa\u2122 Chemically Defined Medium<\/li>\n<li data-rm-block-id=\"block-29\">After 48 hours: 1% FBS + PurMa\u2122 Chemically Defined Medium<\/li>\n<li data-rm-block-id=\"block-30\">After 24 hours: 0% FBS, <strong>PurMa chemically defined RPMI 1640<\/strong> only<\/li>\n<\/ul>\n<p>At each step: Spin down cells, fully discard the spent medium and replace with fresh medium containing the next lower FBS level. You do not need to start with an extremely high cell density; around 500,000 cells\/ml is usually sufficient. Use more than one plate or flask during adaptation, since a small proportion may experience stress or contamination.<\/p>\n<p><strong>Long-Term Culture and Freezing<\/strong><\/p>\n<ol>\n<li data-rm-block-id=\"block-37\">Once cells are fully adapted to <strong>PurMa chemically defined medium RPMI 1640<\/strong>, they should remain in the same chemically defined formulation and vendor for routine culture and cryopreservation.<\/li>\n<li data-rm-block-id=\"block-38\">Expand adapted cells and prepare multiple plates for freezing.<\/li>\n<li data-rm-block-id=\"block-39\">When thawing adapted cells during the first week, supplement <strong>PurMa chemically defined RPMI 1640<\/strong> with 10% PurMa\u2122 CD Booster (Cat# P3C10144) to support recovery.<\/li>\n<li data-rm-block-id=\"block-40\">Maintain cells exclusively in PurMa chemically defined medium after adaptation to preserve phenotype and performance.<\/li>\n<li data-rm-block-id=\"block-41\">This workflow supports stable, long-term, serum-free culture suitable for reproducible experiments and regulated environments.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>The CD-RPMI-1640 includes a base formulation including L-Alanine -L glutamine : <a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Chemically-Defined-RPMI-1640-Formulation-P3C310109-3.pdf\"><span style=\"color: #0000ff;\"><strong>Base formulation of Chemically Defined RPMI-1640 , P3C310109<\/strong><\/span><\/a><\/p>\n<p data-rm-block-id=\"block-45\">\u00a0 \u00a0 \u00a0 \u00a0 <strong>\u00a0FAQs<\/strong><\/p>\n<ul>\n<li data-rm-block-id=\"block-46\"><\/li>\n<li data-rm-block-id=\"block-47\"><strong>Q1. What is PurMa chemically defined RPMI 1640?<\/strong><br \/>\n<strong>A1.<\/strong> PurMa chemically defined RPMI 1640 is a fully synthetic, serum-free version of RPMI-1640 designed for mammalian cell culture. It contains only chemically defined components and a proprietary synthetic growth factor cocktail, with no FBS or animal-derived materials.<\/li>\n<li data-rm-block-id=\"block-48\"><strong>Q2. Does PurMa chemically defined RPMI 1640 contain any FBS or animal components?<\/strong><br \/>\n<strong>A2.<\/strong> No. PurMa chemically defined RPMI 1640 is completely FBS-free and animal component\u2013free. All nutrients, growth factors, and hormones are synthetic and chemically defined, which supports regulatory compliance and reduces lot-to-lot variability.<\/li>\n<li data-rm-block-id=\"block-49\"><strong>Q3. Why does this medium use L\u2011alanyl\u2011L\u2011glutamine instead of L\u2011glutamine?<\/strong><br \/>\n<strong>A3.<\/strong> L\u2011alanyl\u2011L\u2011glutamine is more stable than free L\u2011glutamine. It slows glutamine degradation, extends medium shelf life, and improves buffering capacity. This helps maintain a more stable culture environment and can reduce contamination risks related to pH shifts and breakdown products.<\/li>\n<li data-rm-block-id=\"block-50\"><strong>Q4. How do I transition cells from 10% FBS RPMI to PurMa chemically defined RPMI 1640?<\/strong><br \/>\n<strong>A4.<\/strong> Transition cells gradually by reducing FBS in steps. Start with 10% FBS in PurMa chemically defined medium, then lower FBS to 8%, 6%, 4%, 2%, and 1% every 48 hours, spinning down and fully replacing the medium each time. Finally, move to 0% FBS and use only PurMa chemically defined RPMI 1640. Keep parallel control cultures in standard 10% FBS medium.<\/li>\n<li data-rm-block-id=\"block-51\"><strong>Q5. Do I need booster supplements with PurMa chemically defined RPMI 1640?<\/strong><br \/>\n<strong>A5.<\/strong> For routine culture of adapted cells, PurMa chemically defined RPMI 1640 is used as supplied. During the first week after thawing adapted cells, we recommend adding 10% PurMa CD Booster (Cat# P3C10144) to support robust recovery.<\/li>\n<li data-rm-block-id=\"block-52\"><strong>Q6. What applications is PurMa chemically defined RPMI 1640 suitable for?<\/strong><br \/>\n<strong>A6.<\/strong> PurMa chemically defined RPMI 1640 is ideal for serum-free mammalian cell culture, mechanistic studies requiring low background, assay development, bioproduction optimization, and projects that demand high reproducibility and simplified regulatory documentation due to its fully defined, animal-free composition.<\/li>\n<li data-rm-block-id=\"block-53\"><strong>Q7. What is RPMI- <\/strong><strong>1640<\/strong><strong>\u00a0used for in cell culture?<\/strong><br \/>\n<strong>A7.<\/strong> 1640 medium rpmi is widely used for mammalian cell culture, supporting lymphocytes, hybridomas, and many suspension and adherent cell lines. In its chemically defined form, such as PurMa chemically defined RPMI 1640, it enables serum-free, animal component\u2013free culture with improved reproducibility.<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-55\"><strong>References<\/strong><\/p>\n<ol>\n<li data-rm-block-id=\"block-56\"><span style=\"color: #0000ff;\"><a class=\"docsum-title\" style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/11027156\/\" data-ga-category=\"result_click\" data-ga-action=\"16\" data-ga-label=\"11027156\" data-full-article-url=\"from_term=chemically+defined+medium&amp;from_page=2&amp;from_pos=6\" data-article-id=\"11027156\" rel=\"nofollow noopener\" target=\"_blank\">Growth of NS0 cells in protein-free, chemically defined medium. <\/a><\/span><span class=\"docsum-authors full-authors\">Gorfien S, et al,<span class=\"docsum-journal-citation full-journal-citation\">Biotechnol Prog. 2000 Sep-Oct;16(5):682-7. doi: 10.1021\/bp000109a.<\/span> <span class=\"citation-part\">PMID: <span class=\"docsum-pmid\">11027156<\/span><\/span><\/span><\/li>\n<li data-rm-block-id=\"block-57\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/4960081\/\" rel=\"nofollow noopener\" target=\"_blank\">Culture of normal human leukocytes. <\/a><\/span>Moore GE, et al. 1967 Feb 20;199(8):519-24. PMID: 4960081<\/li>\n<li data-rm-block-id=\"block-58\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/5437104\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle.<\/span> <\/a>Dunne HW, et al. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.<\/li>\n<li data-rm-block-id=\"block-59\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/5274436\/\" rel=\"nofollow noopener\" target=\"_blank\">Long-term culture of human leukaemic leukocytes. <\/a><\/span>P\u00f6ssnerov\u00e1 V, et al. 1970;17(5):513-23. PMID: 5274436<\/li>\n<\/ol>\n<ul>\n<li class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\" data-rm-block-id=\"block-60\">Parameter<\/th>\n<th align=\"left\" data-rm-block-id=\"block-61\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-62\">Appearance<\/td>\n<td align=\"left\" data-rm-block-id=\"block-63\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-64\">pH<\/td>\n<td align=\"left\" data-rm-block-id=\"block-65\">\u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-66\">Osmolality<\/td>\n<td align=\"left\" data-rm-block-id=\"block-67\">\u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-68\">Endotoxin<\/td>\n<td align=\"left\" data-rm-block-id=\"block-69\">\u00a0NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-70\">Mycoplasma<\/td>\n<td align=\"left\" data-rm-block-id=\"block-71\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-72\">Suitability<\/td>\n<td align=\"left\" data-rm-block-id=\"block-73\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-74\">Additive<\/td>\n<td align=\"left\" data-rm-block-id=\"block-75\">\u00a0Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-76\">Indicator<\/td>\n<td align=\"left\" data-rm-block-id=\"block-77\">\u00a0Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-78\">Mycoplasma Detection<\/td>\n<td align=\"left\" data-rm-block-id=\"block-79\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-80\">Sterility Tested<\/td>\n<td align=\"left\" data-rm-block-id=\"block-81\">\u00a0Sterile filtered using 0.1 \u00b5m filter, 3 Times<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-82\">Form<\/td>\n<td align=\"left\" data-rm-block-id=\"block-83\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-84\">Shipping Condition<\/td>\n<td align=\"left\" data-rm-block-id=\"block-85\">\u00a0Room temperature, Overnight<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li data-rm-block-id=\"block-86\"><\/li>\n<\/ul>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-rpmi-1640\/\">What Is RPMI 1640?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>PurMa\u2122 Chemically Defined Roswell-Park Memorial Institute 1640 (CD-RPMI-1640) is a 100% synthetic, FBS-free medium designed for mammalian cell culture. This <strong>PurMa chemically defined RPMI &#8211; 1640<\/strong> formulation contains no animal-derived components and replaces unstable L\u2011glutamine with L\u2011alanyl\u2011L\u2011glutamine for extended shelf life and improved buffering capacity. A proprietary mix of synthetic growth factors and nutrients supports robust growth while delivering high lot-to-lot consistency, ideal for regulatory-sensitive applications and reproducible cell-based assays.<\/p>\n","protected":false},"featured_media":124426,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,512],"product_tag":[596,581,668,666,669,665,667],"class_list":["post-104671","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-roswell-park-memorial-institute-1640","product_tag-advanced","product_tag-cell-culture","product_tag-human-transferrin","product_tag-l-alanyl-l-glutamine","product_tag-recombinant-insulin","product_tag-rpmi-1640","product_tag-serum-reduction","entry","has-media","first","instock","taxable","shipping-taxable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/104671","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=104671"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/124426"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=104671"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=104671"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=104671"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=104671"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}