{"id":105015,"date":"2024-07-10T19:11:11","date_gmt":"2024-07-11T01:11:11","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=105015"},"modified":"2026-07-24T15:22:44","modified_gmt":"2026-07-24T21:22:44","slug":"mccoys-5a-modified-media-m5amm","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/mccoys-5a-modified-media-m5amm\/","title":{"rendered":"McCoy\u2019s 5A Modified Media (M5AMM)"},"content":{"rendered":"<h2>Background<\/h2>\n<p>McCoy\u2019s 5A Modified Medium (M5AMM) was originally used for Walker Carcinosarcoma 256 Cells. It was subsequently modified to create a new medium known as McCoy\u2019s 5A. Additionally, McCoy\u2019s 5A Modified Media (M5AMM) contains several folds of amino acids and vitamins compared to the original formulation. Our McCoy\u2019s 5A covers the main needs for your cell lines, delivers consistency, excellent quality, and the full control you need for mammalian cell culture.<\/p>\n<p>MCCOY\u2019S 5A Contains:<\/p>\n<ul>\n<li>High glucose (3g\/L)<\/li>\n<li>Non-Essential Amino Acids<\/li>\n<li>Phenol Red<\/li>\n<li>L-glutamine<\/li>\n<\/ul>\n<h3>Why PurMa Biologics? Why we stand behind our cell culture media:<\/h3>\n<p>Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize 99% pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.<\/p>\n<h3>Application<\/h3>\n<p>McCoy\u2019s 5A Modified Media (M5AMM) is widely used for commercial cell lines such as CHO, HEK 293, HeLa, as well as Jurkat cells. Moreover, PurMa<sup>TM<\/sup> McCoy\u2019s Medium contains no proteins, lipids, or growth factors. Therefore, McCoy\u2019s 5A Modified Media (M5AMM) requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).<\/p>\n<h3>Formulation<\/h3>\n<p>complete formulation is available in formulation tab as well as here:<strong><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/McCoys-5A-Formulation-P3S010724.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"color: #0000ff;\">McCoy&#8217;s 5A Formulation<\/span><\/a><\/strong><\/p>\n<h4>References<\/h4>\n<ol>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/172190\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Investigations on in vitro survival and virulence of T. pallidum under aerobiosis.<\/span> <\/a>Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136\/sti.51.5.296. PMID: 172190<\/li>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/6413389\/\" rel=\"nofollow noopener\" target=\"_blank\">Mycoplasmal infection of insect cell cultures. <\/a><\/span>Steiner et al.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007\/BF02628958.PMID: 6413389.<\/li>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/2980399\/\" rel=\"nofollow noopener\" target=\"_blank\">In vitro culture of postimplantation hamster embryos. <\/a><\/span>Ebron-et al. Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016\/s0890-6238(88)80006-6.PMID: 2980399<\/li>\n<\/ol>\n<div class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\">Parameter<\/th>\n<th align=\"left\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\">Appearance<\/td>\n<td align=\"left\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">pH<\/td>\n<td align=\"left\">\u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Osmolality<\/td>\n<td align=\"left\">\u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Endotoxin<\/td>\n<td align=\"left\">\u00a0NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Mycoplasma<\/td>\n<td align=\"left\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Suitability<\/td>\n<td align=\"left\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Additive<\/td>\n<td align=\"left\">\u00a0Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Indicator<\/td>\n<td align=\"left\">\u00a0Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Mycoplasma Detection<\/td>\n<td align=\"left\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Sterility Tested<\/td>\n<td align=\"left\">\u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Form<\/td>\n<td align=\"left\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Shipping Condition<\/td>\n<td align=\"left\">\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>&nbsp;<\/p>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-mccoys-5a\/\">What Is McCoy&#8217;s 5A?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>McCoy\u2019s 5A Modified Media (M5AMM) is widely used cor tumor cells. Additionally,\u00a0 modified version of this media\u00a0 contains several folds of amino acids and vitamins compared to the original formulation.<\/p>\n","protected":false},"featured_media":127438,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,599],"product_tag":[581,682,686,556,677,545,594],"class_list":["post-105015","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-mccoys-5a-modified-media-m5amm","product_tag-cell-culture","product_tag-glucose","product_tag-l-glutamine","product_tag-mammalian-cells","product_tag-mccoys-5a","product_tag-phenol-red","product_tag-standard","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/105015","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=105015"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127438"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=105015"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=105015"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=105015"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=105015"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}