William’s E Medium is a modified version of Williams\u2032 Medium D by Williams and Gunn. Although it is like MEM, there is a difference in glucose as well as amino acid contents.\u00a0 This media was originally used to isolate and culture primary liver epithelial cells, distinguishing epithelial-like cells from fibroblast-like cells in culture. It has been shown that this medium is suited for longer term adult primary cell culture.<\/p>\n
PurMa Biologics manufactures 112 types of William’s E Medium which is certainly the most comprehensive catalog of media.<\/p>\n
Why PurMa Biologics? Why we stand behind our cell culture media:<\/strong><\/p>\n Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize 99% pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.<\/p>\n Application of William’s E Medium<\/strong><\/p>\n This media has elements supporting long term culturing of primary cells. It utilizes a bicarbonate buffering system. This media has been used for primary as well as commercial cell lines including: primary liver epithelial cells, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, as well as hybridoma cells.<\/p>\n Formulation:<\/strong><\/p>\n The complete formulation is available in formulation tab as well here:William’s E Medium Formulation<\/strong><\/a><\/span><\/p>\n <\/p>\n <\/p>\nReferences<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n