![\"Chemically](\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Chemically-Defined-William-E-Graph-1080x590.jpg\")
<\/p>\nPurMa\u2122 Chemically Defined Williams\u2019 E Medium (CD Williams\u2019 E Medium) is a proprietary formulation used to grow cells in vitro without any undefined or animal-derived components. Moreover,\u00a0CD Williams\u2019 E Medium contains known quantities of pure chemicals, including amino acids, vitamins, salts, sugars, and more importantly a proprietary combination of synthetic growth factors.<\/p>\n
It is absolutely crucial to mention that the\u00a0PurMa\u2122 Growth Supplement for each conventional media such as DMEM, IMDM, RPMI-1640\u2026 (base media) are slightly different in combination, amount of several growth factors, ions, chemicals, pH with particular adjustments needs to be done, based on the environments the base media provides.<\/p>\n
This provides a consistent and controlled environment for cell growth and research. Noticeably, CD Williams\u2019 E Medium is essential for applications that require reproducibility and regulatory compliance as well as for studying specific cellular processes, without extraneous variables. Furthermore, CD Williams\u2019 E Medium is designed for custom formulations tailored for various cell types<\/p>\n
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It is crucially important to have a gradual transition from media containing FBS to chemically defined cell culture media. Follow these steps:<\/p>\n
Importantly, for each change, spin down and fully discard the media and replace it with the next lower percentage of FBS.<\/p>\n
Noticeably, you don\u2019t have to start with a huge number of cells (~500,000 cells\/ml). Additionally, we strongly recommend you start with more than one plate as some of the plates might get contaminated<\/p>\n
So, once the cells are kept in chemically defined Media, you should always keep them in the same chemically defined medium from the same vendor and the same formulation, even for freezing (see below).<\/p>\n
Usually, after you get your cells adapted to PurMa\u2122 <\/sup>Chemically Defined Medium you need to make several plates and freeze down several vials. The mentioned cells are adapted to chemically defined medium and when you thaw them in the first week, in addition to PurMa\u2122 Chemically Defined Medium, you need to add 10% PurMa\u2122 <\/sup>CD Booster (Cat# P3C10144).<\/p>\n The CD Williams\u2019 E Medium includes a base formulation including L-Alanine -L glutamine : Base Formulation of CD William’s E Medium, P3C310880<\/strong><\/span><\/a><\/p>\n Additionally, CD Williams\u2019 E Medium contains a proprietary synthetic cocktail of specific growth factors, hormones, and other nutrients which makes it independent of any serum and any trace of animal products.<\/p>\n <\/p>\nFormulation<\/h2>\n
References<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n Sterile filtered using 0.1 \u00b5m filter, 3 Times<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature, Overnight<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n