Earle’s Balanced Salt Solution (EBSS) is an isotonic buffer solution. It, however, contains inorganic salts and carbohydrates as an energy source designed for the short-term maintenance of mammalian cells in a CO2 environment. The solution additionally provides an ideal environment to preserve the structural and physiological integrity of cells invitro<\/em> by maintaining pH as well as the balance between intra and extracellular osmotic pressure.<\/p>\n Not sure whether to choose HBSS or EBSS? See our comparison guide<\/a>.<\/p>\n EBSS has an especially excellent role in protocols requiring the use of consistent conditions such as crosslinking, biotinylation, and fluorescent labeling reactions which require an amine-free buffer.<\/p>\n For complete formulation, click here: EBSS Formulation<\/span><\/a><\/strong><\/p>\n <\/p>\n PurMa Biologics manufactures Regular grade of buffer by using:\u00a0 PurMa\u2122 Cell Culture Suited Water (Cat# P3W110101)<\/a><\/p>\n We sterilize the buffers by autoclaving the solution. Finally we pack the buffer in our Class 100 Aseptic Facility.<\/p>\n PurMa Biologics manufactures this grade of buffer by using :PurMa\u2122 <\/a>Cell Culture Suited Water (Cat# P3W110101)<\/a><\/p>\n After adjusting the pH, we finally, sterilize the buffer by\u00a0 3 times passing through 0.2 \u00b5m filters. Additionally,\u00a0 We pack the buffer under N2 gas\u00a0 in our Class 100 Aseptic Facility.<\/p>\n PurMa Biologics manufactures this grade of buffer by using :PurMa\u2122 Ultra-Pure Cell Culture Suited Water (Cat# P3W110102) <\/a><\/p>\n We sterilize the buffers by autoclaving the solution.\u00a0 Additionally, we pass the buffer\u00a0 three times through 0.1 \u00b5m filters,\u00a0 and finally\u00a0 pack it under N2 gas in our Class 100 Aseptic Facility.<\/p>\n PurMa Biologics manufactures this grade of buffer by using: PurMa\u2122 Ultra-Pure Cell Culture Suited Water (Cat# P3W110102) <\/a><\/p>\n After depleting\u00a0 the endotoxins (see below for more details), we sterilize the buffer by 3 times passing through\u00a0 0.1 \u00b5m filters and pack under N2 gas in our Class 100 Aseptic Facility<\/p>\n PurMa Biologics has the most sophisticated endotoxin removal procedure. To remove this pathogen, we pass the buffer \u00a0through the PurMa\u2122 Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column,\u00a0 we covalently conjugate Sepharose 6B\u00a0 to an endotoxin-specific absorbent. We finally\u00a0 eliminate the endotoxin residual by passing the unbound fraction three times through 0.1\u00b5m filters.<\/p>\n References<\/span><\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" An isotonic balanced salt solution for short-term maintenance of mammalian cells in a CO2 environment.<\/p>\nGeneral Features<\/h2>\n
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Specific Features<\/h2>\n
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Application<\/h2>\n
Formulation<\/h2>\n
PurMa Biologics Manufactures Several Grades of Buffers<\/strong><\/h3>\n
Regular Grade Buffer<\/strong><\/h3>\n
Cell Culture Suited Grade Buffer<\/strong><\/h3>\n
RNase and DNase Free Grade Buffer<\/strong><\/h3>\n
Cell Culture Suited \/ Endotoxin Depleted Grade Buffer<\/strong><\/h3>\n
The Endotoxin Removing Procedure from Buffer<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n \u00a0Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.2 \u00b1 0.1; 7.4 \u00b1 0.1; 7.6 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n \u00a0Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0N\/A<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n \u00a0Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n \u00a0Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n \n