![\"Chemically](\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/CDLL15-graph-Image-1080x618.png\")
<\/strong><\/p>\nImportant Notes:<\/h2>\n
It is crucially vital, for a gradual transition from media containing FBS to chemically defined cell culture media, to follow these steps:<\/p>\n
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- Starting Point: 10% FBS+ (control) (you need two controls, one to be left in only 10% FBS and continue monitoring for comparing with 0% FBS + PurMa\u2122 <\/sup>Chemically Defined Medium. The second one chemically defined medium + 10% FBS which is going through the reduction of FBS process)<\/li>\n
- 48 hours later: 8% FBS in + PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n
- 48 hours later: 6% FBS in + PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n
- 48 hours later: 4% FBS+ PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n
- 48 hours later: 2% FBS+ PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n
- 48 hours later: 1% FBS + PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n
- 24 hours later: 0% FBS and only PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n<\/ul>\n
Importantly, for each change, spin down and fully discard the media and replace it with the less percentages of FBS.<\/p>\n
Noticeably, you don\u2019t have to start with a huge number of cells (~500,000 cells\/ml). Additionally, we strongly recommend you start with more than one plate as some of the plates might get contaminated<\/p>\n
So, once the cells are kept in chemically defined Media, you should always keep them in the same chemically defined medium from the same vendor and the same formulation, even for freezing (see below).<\/p>\n
Usually, after you get your cells adapted to PurMa\u2122 <\/sup>Chemically Defined Medium you need to make several plates and freeze down several vials. The mentioned cells are adapted to chemically defined medium and when you thaw them in the first week, in addition to PurMa\u2122 Chemically Defined Medium, you need to add 10% PurMa\u2122 <\/sup>CD Booster (Cat# P3C10144).<\/p>\n
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Formulation<\/h2>\n
The (CD-LL15)includes a base formulation including L-Alanine -L glutamine : Base Formulation of Leibovitz\u2019s L-15 Medium, P3C310613<\/a><\/strong><\/span><\/p>\n
Additionally, (CD-LL-15) contains a proprietary synthetic cocktail of specific growth factors, hormones, and other nutrients which makes it independent of any serum and any trace of animal products.<\/p>\n
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References<\/h2>\n
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- 48 hours later: 8% FBS in + PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n