Ian McPherson and Michael Stoker developed Glasgow\u2019s Modified Eagle\u2019s Medium. They used polyoma virus to transform four fibroblast clones from a culture of baby hamster kidney cells.\u00a0 As a result of particular criteria mentioned below, Glasgow Minimum Essential Medium (GMEM) is used without serum. It needs to be supplemented with 10% Tryptose phosphate broth.<\/p>\n
Moreover, it contains twice amount of amino acids and vitamins compared to the original Basal Medium Eagle.<\/p>\n
PurMa Biologics manufactures 77 types of GMEM<\/p>\n
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize 99% pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.<\/p>\n
It is suitable for growth of primate embryonic stem cells, MDCK, NS0, BHK-21 as well as CHO cells containing the GS expression system.<\/p>\n
As an alternative to 10% Tryptose phosphate broth, we strongly recommend PurMa TM 50X GS Supplement. This is certainly an enriched mixture of amino acids and nucleosides, and Fetal Bovine Serum (FBS) (Cat# P3S528332). Furthermore, this media has been formulated for use in a 5% carbon dioxide atmosphere. As a result, it utilizes a bicarbonate buffering system.<\/p>\n
The Complete formulation is available in ” formulation tab” as well as by clicking here: Glasgow\u2019s (GMEM) Formulation<\/span><\/strong><\/a><\/p>\n <\/p>\nReferences<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n