Minimum Essential Media (MEM) In Hanks\u2019 Buffer is a modification of Basal Medium Eagle. It was originally developed by Harry Eagle for specific\u00a0 requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle\u2019s buffer for use in a CO2<\/sub>\u00a0incubator, or with Hanks’ salts for use without CO2<\/sub>.<\/p>\n Hanks buffer, it contains:<\/p>\n Moreover, PurMa Biologics manufactures 122 types of MEM including several types of essential media MEM in Hanks’<\/p>\n Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize 99% pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.<\/p>\n Minimum Essential Media MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).<\/p>\n Complete formulation click here as well as in Formulation Tab: MEM (HBSS) Formulation in Hanks’ Buffer<\/strong><\/a><\/span><\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" Minimum Essential Media (MEM) In Hanks\u2019 Buffer is a modification of Basal Medium Eagle. This media includes higher concentrations of amino acids making it suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures two types of media, MEM in hanks buffer as well as MEM with Earle\u2019s buffer for use in a CO2<\/sub>\u00a0incubator, or with Hanks\u2019 salts for use without CO2<\/sub>.<\/p>\n","protected":false},"featured_media":127442,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,605],"product_tag":[581,523,515,545,594],"class_list":["post-109774","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-minimum-essential-media-mem-in-hanks-buffer","product_tag-cell-culture","product_tag-hanks-buffer","product_tag-hbss","product_tag-phenol-red","product_tag-standard","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/109774","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=109774"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127442"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=109774"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=109774"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=109774"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=109774"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}PurMa\u2122<\/sup> MEM media in Hank\u2019s buffer includes Hank’s buffer<\/h2>\n
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Additionally, Minimum Essential Media (MEM) In Hanks\u2019 Buffer contains:<\/h2>\n
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Why PurMa Biologics? Why we stand behind our cell culture media<\/h2>\n
Applications<\/h2>\n
Formulation<\/h2>\n
References<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n N\/A<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n