{"id":110329,"date":"2024-07-17T09:01:23","date_gmt":"2024-07-17T15:01:23","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=110329"},"modified":"2026-07-31T12:43:43","modified_gmt":"2026-07-31T18:43:43","slug":"purma-biologics-mem-alpha-modification-in-earles-buffer-mem-%ce%b1","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/purma-biologics-mem-alpha-modification-in-earles-buffer-mem-%ce%b1\/","title":{"rendered":"MEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1)"},"content":{"rendered":"<h2 data-rm-block-id=\"block-1\">Background<\/h2>\n<p data-rm-block-id=\"block-2\">MEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1) is certainly a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM -\u03b1 Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.<\/p>\n<h2 data-rm-block-id=\"block-3\">MEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1)\u00a0 contains<\/h2>\n<ul>\n<li data-rm-block-id=\"block-4\">Non-essential amino acids.<\/li>\n<li data-rm-block-id=\"block-5\">Sodium pyruvate.<\/li>\n<li data-rm-block-id=\"block-6\">Lipoic acid.<\/li>\n<li data-rm-block-id=\"block-7\">Vitamin B12.<\/li>\n<li data-rm-block-id=\"block-8\">Biotin and,<\/li>\n<li data-rm-block-id=\"block-9\">Ascorbic acid.<\/li>\n<li data-rm-block-id=\"block-10\">Also, MEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1) does not contain deoxyribonucleosides and ribonucleosides.<\/li>\n<li data-rm-block-id=\"block-11\">Moreover, PurMa<sup>TM<\/sup> MEM-\u03b1 media in Earle&#8217;s buffer includes Earles\u2019 buffer<\/li>\n<\/ul>\n<h2 data-rm-block-id=\"block-12\">The composition of Earle&#8217;s buffer, it contains:<\/h2>\n<ol>\n<li data-rm-block-id=\"block-13\">Calcium chloride<\/li>\n<li data-rm-block-id=\"block-14\">Magnesium sulfate<\/li>\n<li data-rm-block-id=\"block-15\">Potassium chloride<\/li>\n<li data-rm-block-id=\"block-16\">Sodium bicarbonate<\/li>\n<li data-rm-block-id=\"block-17\">Sodium chloride<\/li>\n<li data-rm-block-id=\"block-18\">Sodium Phosphate, monobasic<\/li>\n<\/ol>\n<h2 data-rm-block-id=\"block-19\">Additionally, MEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1)<\/h2>\n<p data-rm-block-id=\"block-20\">it contains:<\/p>\n<ul>\n<li data-rm-block-id=\"block-21\">Low level of glucose (1.00 g\/ liter)<\/li>\n<li data-rm-block-id=\"block-22\">More importantly, Sodium bicarbonate (2.2 g\/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.<\/li>\n<li data-rm-block-id=\"block-23\">2 mM L-glutamine (292 mg\/L)<\/li>\n<li data-rm-block-id=\"block-24\">Also, PurMa Biologics manufactures 118 types of MEM-\u03b1<\/li>\n<\/ul>\n<h2 data-rm-block-id=\"block-25\">Application<\/h2>\n<p data-rm-block-id=\"block-26\">This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes as well as human melanoma cells. Furthermore, MEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1) has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast.<\/p>\n<h2 data-rm-block-id=\"block-27\">Why PurMa Biologics? Why we stand behind our cell culture media.<\/h2>\n<p data-rm-block-id=\"block-28\">Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, we utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.<\/p>\n<h2 data-rm-block-id=\"block-29\">Formulation<\/h2>\n<p data-rm-block-id=\"block-30\">Complete formulation is in the &#8221; Formulation tab&#8221; as well as here: <a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/MEM-alpha-Formulation-in-Earls-Buffer-P3S310123.pdf\" target=\"_blank\" rel=\"noopener\"><strong><span style=\"color: #0000ff;\">MEM-alpha Formulation in Earle&#8217;s Buffer<\/span><\/strong><\/a><\/p>\n<h2 data-rm-block-id=\"block-31\">References<\/h2>\n<ol>\n<li data-rm-block-id=\"block-32\"><span style=\"color: #0000ff;\">The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium.<\/span> Keay et al.\u00a0 Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).<\/li>\n<li data-rm-block-id=\"block-33\"><span style=\"color: #0000ff;\">Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. <\/span>Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)<\/li>\n<\/ol>\n<div class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\" data-rm-block-id=\"block-34\">Parameter<\/th>\n<th align=\"left\" data-rm-block-id=\"block-35\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-36\">Appearance<\/td>\n<td align=\"left\" data-rm-block-id=\"block-37\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-38\">pH<\/td>\n<td align=\"left\" data-rm-block-id=\"block-39\">7.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-40\">Osmolality<\/td>\n<td align=\"left\" data-rm-block-id=\"block-41\">275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-42\">Endotoxin<\/td>\n<td align=\"left\" data-rm-block-id=\"block-43\">NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-44\">Mycoplasma<\/td>\n<td align=\"left\" data-rm-block-id=\"block-45\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-46\">Suitability<\/td>\n<td align=\"left\" data-rm-block-id=\"block-47\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-48\">Additive<\/td>\n<td align=\"left\" data-rm-block-id=\"block-49\">Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-50\">Indicator<\/td>\n<td align=\"left\" data-rm-block-id=\"block-51\">Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-52\">Mycoplasma Detection<\/td>\n<td align=\"left\" data-rm-block-id=\"block-53\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-54\">Sterility Tested<\/td>\n<td align=\"left\" data-rm-block-id=\"block-55\">Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-56\">Form<\/td>\n<td align=\"left\" data-rm-block-id=\"block-57\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-58\">Shipping Condition<\/td>\n<td align=\"left\" data-rm-block-id=\"block-59\">\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p data-rm-block-id=\"block-60\">\n","protected":false},"excerpt":{"rendered":"<p>MEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. Scientist around the globe use MEM -\u03b1 Modification for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN)<\/p>\n","protected":false},"featured_media":127444,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,604],"product_tag":[581,519,520,682,527,517,522,545,683,594,546],"class_list":["post-110329","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-mem-alpha-modification-in-earles-buffer-mem-","product_tag-cell-culture","product_tag-earles-buffer","product_tag-ebss","product_tag-glucose","product_tag-mem","product_tag-mem-alpha","product_tag-mem-","product_tag-phenol-red","product_tag-sodium-bicarbonate","product_tag-standard","product_tag-without-phenol-red","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/110329","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=110329"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127444"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=110329"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=110329"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=110329"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=110329"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}