{"id":110695,"date":"2024-07-17T21:37:47","date_gmt":"2024-07-18T03:37:47","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=110695"},"modified":"2026-07-23T10:49:50","modified_gmt":"2026-07-23T16:49:50","slug":"mem-alpha-hanks-buffer","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/mem-alpha-hanks-buffer\/","title":{"rendered":"MEM Alpha Modification (MEM-\u03b1) in Hanks\u2019 Buffer"},"content":{"rendered":"<h2>Background<\/h2>\n<p>MEM Alpha Modification (MEM-\u03b1) in Hanks\u2019 Buffer is certainly a suitable choice for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells.\u00a0 Furthermore, eliminating calcium in this media facilitates the growth of cells in suspension cultures.<\/p>\n<h2>Ingredients<\/h2>\n<p>MEM -\u03b1 has improved regular MEM as it contains:<\/p>\n<ul>\n<li>Non-essential amino acids.<\/li>\n<li>Sodium pyruvate.<\/li>\n<li>Lipoic acid.<\/li>\n<li>Vitamin B12.<\/li>\n<li>Biotin and,<\/li>\n<li>Ascorbic acid.<\/li>\n<li>Also, MEM-\u03b1 does not contain deoxyribonucleosides and ribonucleosides.<\/li>\n<\/ul>\n<h2>PurMa\u2122 MEM -\u03b1 media in Hank\u2019s buffer includes HBSS<\/h2>\n<p>Composition of HBSS buffer contains:<\/p>\n<ul>\n<li>Potassium Chloride<\/li>\n<li>Potassium Phosphate, monobasic<\/li>\n<li>Sodium Bicarbonate<\/li>\n<li>Sodium Chloride<\/li>\n<li>Sodium Phosphate, dibasic<\/li>\n<li>Low level of glucose (1.00 g\/ liter)<\/li>\n<li>Sodium bicarbonate (0.35 g\/L)<\/li>\n<li>2 mM L-glutamine (292 mg\/L)<\/li>\n<li>Low level of glucose (1.00 g\/ liter)<\/li>\n<\/ul>\n<p>Furthermore, PurMa Biologics manufactures 123 types of MEM with HBSS buffer<\/p>\n<h2>Why PurMa Biologics? Why we stand behind our cell culture media<\/h2>\n<p>Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.<\/p>\n<h2>Application<\/h2>\n<p>Alpha modification of Minimum Essential Medium Eagle certainly makes an excellent choice for the isolation of mesenchymal stem cells, adipose-derived stem cells as well as osteoblast. MEM -\u03b1 Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.<\/p>\n<h2>Formulation<\/h2>\n<p>to get access to detailed formulation visit &#8221; formulation Tab&#8221; as well as by clicking here :<a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/MEM-alpha-Formulation-in-Hanks-Buffer-HBSS-P3S410123.pdf\" target=\"_blank\" rel=\"noopener\"><strong><span style=\"color: #0000ff;\"> MEM-alpha Formulation in Hank&#8217;s Buffer (HBSS)<\/span><\/strong><\/a><\/p>\n<h2>References<\/h2>\n<ol>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/tech-docs\/paper\/485260\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium<\/span>.<\/a> Keay L.\u00a0 Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).<\/li>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/tech-docs\/paper\/1471873\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction.<\/span><\/a> Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<div class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\">Parameter<\/th>\n<th align=\"left\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\">Appearance<\/td>\n<td align=\"left\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">pH<\/td>\n<td align=\"left\">7.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Osmolality<\/td>\n<td align=\"left\">275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Endotoxin<\/td>\n<td align=\"left\">NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Mycoplasma<\/td>\n<td align=\"left\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Suitability<\/td>\n<td align=\"left\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Additive<\/td>\n<td align=\"left\">Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Indicator<\/td>\n<td align=\"left\">Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Mycoplasma Detection<\/td>\n<td align=\"left\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Sterility Tested<\/td>\n<td align=\"left\">Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Form<\/td>\n<td align=\"left\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Shipping Condition<\/td>\n<td align=\"left\">\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>MEM Alpha Modification (MEM-\u03b1) in Hanks\u2019 Buffer\u00a0 is suitable\u00a0 for mammalian cell culture . Moreover, This media can also be used with a variety of suspension as well as\u00a0 adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells.<\/p>\n","protected":false},"featured_media":127446,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,606],"product_tag":[581,682,523,515,517,522,545,594],"class_list":["post-110695","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-mem-alpha-modification-mem--in-hanks-buffer","product_tag-cell-culture","product_tag-glucose","product_tag-hanks-buffer","product_tag-hbss","product_tag-mem-alpha","product_tag-mem-","product_tag-phenol-red","product_tag-standard","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/110695","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=110695"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127446"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=110695"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=110695"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=110695"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=110695"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}