MEM Alpha with Nucleosides in Earle\u2019s Buffer (MEM-\u03b1-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside, and the formulation is available below. Depending on cell types, laboratories use MEM-\u03b1-N for growing mammalian cells for a variety of suspension as well as adherent cells. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.<\/p>\n
MEM Alpha Modification with Nucleosides in Earle\u2019s Buffer (MEM-\u03b1-N-EBSS) is a modification of MEM.<\/p>\n
As the composition of Earls buffer, it contains:<\/p>\n
it contains:<\/p>\n
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.<\/p>\n
This media can also be used for keratinocytes, primary rat astrocytes, as well as human melanoma cells. MEM Alpha with Nucleosides in Earle\u2019s Buffer (MEM-\u03b1-N-EBSS) has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast. Additionally, PurMa\u2122 MEM Alpha with Nucleosides in Earle\u2019s Buffer (MEM-\u03b1-N-EBSS) facilitates the growth and maintenance of primary osteoblast cultures. furthermore, this media performs well for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.<\/p>\n
Click here for complete formulation as well as in ” Formulation Tab”: MEM alpha with Nucleoside Formulation<\/a><\/strong><\/span><\/p>\n \n","protected":false},"excerpt":{"rendered":" MEM Alpha with Nucleosides in Earle\u2019s Buffer (MEM-\u03b1-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside and, furthermore depending on cell types, laboratories use MEM-\u03b1-N for growing mammalian cells.<\/p>\n","protected":false},"featured_media":127448,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,607],"product_tag":[581,519,520,682,527,517,522,528,545,683,594],"class_list":["post-111767","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-mem-alpha-with-nucleosides-in-earles-buffer-mem--n-ebss","product_tag-cell-culture","product_tag-earles-buffer","product_tag-ebss","product_tag-glucose","product_tag-mem","product_tag-mem-alpha","product_tag-mem-","product_tag-nucleosides","product_tag-phenol-red","product_tag-sodium-bicarbonate","product_tag-standard","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/111767","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=111767"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127448"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=111767"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=111767"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=111767"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=111767"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}References<\/h2>\n
\n
\n\n
\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n