MEM Alpha-Modification with Nucleosides MAX in Earle\u2019s Buffer (MEM-Alpha-N-MAX-EBBS) is a commonly used media for Mammalian cell culture.<\/p>\n
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.<\/p>\n
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.<\/p>\n
In addition to our Max formulation products, we manufacture PurMaTM <\/sup>GluaSup (Cat# P3S210 559), a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.<\/p>\n PurMa\u2122 MEM Alpha-Modification with Nucleosides MAX in Earle\u2019s Buffer (MEM-Alpha-N-MAX-EBBS) is modified from MEM-\u03b1-MAX with the following ingredients:<\/p>\n MEM Alpha-Modification with Nucleosides MAX in Earle\u2019s Buffer (MEM-Alpha-N-MAX-EBBS) contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).<\/p>\n For complete formulation Click here: MEM alpha-MAX with Nucleoside Formulation<\/strong> <\/a><\/span><\/p>\n \n","protected":false},"excerpt":{"rendered":" MEM \u03b1-Modification with Nucleosides MAX in Earle\u2019s Buffer (MEM-\u03b1-N-MAX-EBBS) contains L-Alanyl-L-Glutamine. \u00a0 Additionally, MEM-\u03b1-N-MAX (EBBS) significantly improves cell viability and growth. We manufacture several types of MEM-\u03b1-N-MAX based on your requirements.<\/p>\n","protected":false},"featured_media":127449,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,607],"product_tag":[581,519,520,682,666,556,595,517,522,528,545,683,546],"class_list":["post-112204","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-mem-alpha-with-nucleosides-in-earles-buffer-mem--n-ebss","product_tag-cell-culture","product_tag-earles-buffer","product_tag-ebss","product_tag-glucose","product_tag-l-alanyl-l-glutamine","product_tag-mammalian-cells","product_tag-max","product_tag-mem-alpha","product_tag-mem-","product_tag-nucleosides","product_tag-phenol-red","product_tag-sodium-bicarbonate","product_tag-without-phenol-red","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/112204","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=112204"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127449"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=112204"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=112204"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=112204"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=112204"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Contents<\/h2>\n
MEM Alpha-Modification with Nucleosides MAX in Earle\u2019s Buffer (MEM-Alpha-N-MAX-EBBS) contains:<\/h3>\n
\n
\n
Formulation<\/h2>\n
References<\/h2>\n
\n
\n\n
\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n