MEM \u03b1-Modification with Nucleosides\u00a0 in Hanks\u2019 buffer provides a great nutritional culture environment for mammalian cells.\u00a0 This media also plays a key role in the process of selection of transfected DHFR-negative cells. Moreover, MEM-\u03b1-N-HBSS is widely used for a variety of suspension and adherent cells. Calcium, however, plays a crucial role in media as eliminating this ion facilitates the growth of cells in suspension cultures.<\/p>\n
MEM-\u03b1 with nucleosides is basically the modified version of MEM-\u03b1 and contains:<\/p>\n
The composition of HBSS buffer:<\/p>\n
Additionally, MEM \u03b1-Modification with Nucleosides in Hanks\u2019 Buffer also Contains:<\/p>\n
PurMa Biologics manufactures 119 types of MEM-\u03b1-N-HBSS<\/p>\n
Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize 99% pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.<\/p>\n
Furthermore,\u00a0 MEM-\u03b1-N-HBSS is used for various cell types such as keratinocytes, primary rat astrocytes, as well as human melanoma cells. Also, MEM-\u03b1-N-HBSS has been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblasts. Moreover, MEM \u03b1-Modification is widely used for primary osteoblast cultures.<\/p>\n
For complete formulation\u00a0 click here as well as ” formulation Tab : MEM alpha with nucleoside in HBSS Buffer Formulation<\/a><\/strong><\/span><\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" MEM \u03b1-Modification with Nucleosides in Hanks\u2019 buffer provides a great nutritional culture environment for mammalian cells.\u00a0 this media as well plays a key role in the process of selection for transfected DHFR-negative cells.<\/p>\n","protected":false},"featured_media":127450,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,608],"product_tag":[581,523,515,517,522,528,545,594],"class_list":["post-112443","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-mem--modification-with-nucleosides-in-hanks-buffer","product_tag-cell-culture","product_tag-hanks-buffer","product_tag-hbss","product_tag-mem-alpha","product_tag-mem-","product_tag-nucleosides","product_tag-phenol-red","product_tag-standard","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/112443","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=112443"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127450"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=112443"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=112443"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=112443"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=112443"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}References<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n