MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks\u2019 Buffer is a commonly used media for Mammalian cell culture.<\/p>\n
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.<\/p>\n
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.<\/p>\n
In addition to our Max formulation products, we manufacture PurMaTM <\/sup>GluaSup (Cat# P3S210 559), a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.<\/p>\n PurMa\u2122 MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks\u2019 Buffer is modified from MEM-\u03b1-MAX with the following ingredients:<\/p>\n MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks\u2019 Buffer contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).<\/p>\n The complete formulation can be found here: MEM alpha-MAX with nucleoside in HBSS Buffer Formulation<\/a><\/strong><\/span><\/p>\n \n","protected":false},"excerpt":{"rendered":" MEM \u03b1-Modification with Nucleosides MAX in Hanks\u2019 Buffer (MEM-\u03b1-N-MAX-HBSS) contains L-Alanyl-L-Glutamine, as well as Nucleosides.\u00a0 \u00a0Additionally, MEM-\u03b1-N-MAX (HBSS) significantly improves cell viability and growth. We manufacture several types of MEM-\u03b1-N-MAX based on your requirements.<\/p>\n","protected":false},"featured_media":127451,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,608],"product_tag":[581,682,523,515,666,556,595,517,522,528,545,546],"class_list":["post-112880","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-mem--modification-with-nucleosides-in-hanks-buffer","product_tag-cell-culture","product_tag-glucose","product_tag-hanks-buffer","product_tag-hbss","product_tag-l-alanyl-l-glutamine","product_tag-mammalian-cells","product_tag-max","product_tag-mem-alpha","product_tag-mem-","product_tag-nucleosides","product_tag-phenol-red","product_tag-without-phenol-red","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/112880","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=112880"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127451"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=112880"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=112880"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=112880"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=112880"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Contents<\/h2>\n
MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks\u2019 Buffer contains:<\/h3>\n
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Formulation<\/h2>\n
References<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n