{"id":113144,"date":"2024-07-19T11:50:48","date_gmt":"2024-07-19T17:50:48","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&p=113144"},"modified":"2026-07-31T12:43:43","modified_gmt":"2026-07-31T18:43:43","slug":"basal-medium-eagle-bme-in-hanks-buffer","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/basal-medium-eagle-bme-in-hanks-buffer\/","title":{"rendered":"Basal Medium Eagle (BME) in Hanks\u2019 Buffer"},"content":{"rendered":"

Background<\/h2>\n

Basal Medium Eagle (BME) in Hanks\u2019 Buffer was originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Markedly, BME is the predecessor of Eagle\u2019s Minimum Essential Medium (MEM) and Dulbecco\u2019s Modified Eagle\u2019s Medium (DME). PurMa Biologics presently manufactures BME with Earle\u2019s for use in a CO2<\/sub>\u00a0incubator, or with Hanks’ (HBSS) salts for use without CO2<\/sub>.<\/p>\n

Ingredients<\/h2>\n

As the composition, this media contains HBSS buffer<\/p>\n

HBSS buffer contains:<\/p>\n

    \n
  1. Potassium Chloride<\/li>\n
  2. Potassium Phosphate, monobasic<\/li>\n
  3. Sodium Bicarbonate<\/li>\n
  4. Sodium Chloride<\/li>\n
  5. And lastly, Sodium Phosphate, dibasic<\/li>\n<\/ol>\n

    Application<\/h2>\n

    PurMa Biologics manufactures 117 types of Basal Medium Eagle (BME) in Hanks\u2019 Buffer. This media has been successfully used for a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as \u00a0primary rat astrocytes.<\/p>\n

    Formulation<\/h2>\n

    For complete formulation click, or alternatively \u00a0look at the \u201c Formulation Tab\u201d: \u00a0BME with Hanks’ Buffer (HBSS) Formulation<\/strong><\/a><\/span><\/p>\n

    References<\/h2>\n
      \n
    1. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells.<\/span> <\/a>LEWIS et al. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128\/jb.83.3.475-482.1962. PMID: 14464909<\/li>\n
    2. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. <\/a><\/span>Healy et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128\/am.21.1.1-5.1971.<\/li>\n
    3. Differential cytopathogenicity accompanying Mycoplasma pneumoniae infection of human lung fibroblasts maintained in newborn bovine serum or fetal bovine serum. <\/a><\/span>Upchurch et al. In Vitro. 1983 Mar;19(3 Pt 1):203-9. doi: 10.1007\/BF0 2618060. PMID: 6187665.<\/li>\n<\/ol>\n

      \n

      \n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
      Parameter<\/th>\nSpecification<\/th>\n<\/tr>\n<\/thead>\n
      Appearance<\/td>\nRed, clear liquid<\/td>\n<\/tr>\n
      pH<\/td>\n7.2 \u00b1 0.1<\/td>\n<\/tr>\n
      Osmolality<\/td>\n275-360 mOsm\/L<\/td>\n<\/tr>\n
      Endotoxin<\/td>\nNMT< 2EU\/mL<\/td>\n<\/tr>\n
      Mycoplasma<\/td>\nNegative<\/td>\n<\/tr>\n
      Suitability<\/td>\n\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n
      Additive<\/td>\nN\/A<\/td>\n<\/tr>\n
      Indicator<\/td>\nPhenol red<\/td>\n<\/tr>\n
      Mycoplasma Detection<\/td>\nNegative<\/td>\n<\/tr>\n
      Sterility Tested<\/td>\nSterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n
      Form<\/td>\nLiquid<\/td>\n<\/tr>\n
      Shipping Condition<\/td>\n\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

      \n","protected":false},"excerpt":{"rendered":"

      Basal Medium Eagle (BME) in Hanks\u2019 Buffer was originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME is the predecessor of Eagle\u2019s Minimum Essential Medium (MEM) and Dulbecco\u2019s Modified Eagle\u2019s Medium (DME).<\/p>\n","protected":false},"featured_media":127432,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,610],"product_tag":[581,523,515,545,594],"class_list":["post-113144","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-basal-medium-eagle-bme-in-hanks-buffer","product_tag-cell-culture","product_tag-hanks-buffer","product_tag-hbss","product_tag-phenol-red","product_tag-standard","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/113144","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=113144"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127432"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=113144"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=113144"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=113144"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=113144"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}