Basal Medium Eagle-MAX (BME-MAX) in Hanks\u2019 Buffer is a commonly used media for Mammalian cell culture.<\/p>\n
Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.<\/p>\n
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.<\/p>\n
In addition to our Max formulation products, we manufacture PurMaTM <\/sup>GluaSup (Cat# P3S210 559), a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.<\/p>\n Basal Medium Eagle-MAX (BME-MAX) in Hanks\u2019 Buffer contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).<\/p>\n The complete formulation can be found here: BME-MAX with Hanks’ Buffer (HBSS) Formulation<\/span><\/strong><\/a><\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" Basal Medium Eagle-MAX (BME-MAX) in Hanks\u2019 Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. \u00a0 BME-MAX in HBBS significantly improves cell viability and growth. We manufacture several types of BME-MAX based on your requirements.<\/p>\n","protected":false},"featured_media":127433,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,610],"product_tag":[581,682,523,515,666,556,595,545,546],"class_list":["post-113268","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-basal-medium-eagle-bme-in-hanks-buffer","product_tag-cell-culture","product_tag-glucose","product_tag-hanks-buffer","product_tag-hbss","product_tag-l-alanyl-l-glutamine","product_tag-mammalian-cells","product_tag-max","product_tag-phenol-red","product_tag-without-phenol-red","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/113268","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=113268"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127433"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=113268"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=113268"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=113268"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=113268"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Contents<\/h2>\n
Basal Medium Eagle-MAX (BME-MAX) in Hanks\u2019 Buffer contains:<\/h3>\n
\n
Formulation<\/h2>\n
References<\/h2>\n
\n
\n\n
\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n N\/A<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n