{"id":113833,"date":"2024-07-20T06:44:02","date_gmt":"2024-07-20T12:44:02","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=113833"},"modified":"2026-07-23T10:49:54","modified_gmt":"2026-07-23T16:49:54","slug":"basal-medium-eagle-bme-in-earles-buffer","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/basal-medium-eagle-bme-in-earles-buffer\/","title":{"rendered":"Basal Medium Eagle (BME) in Earle\u2019s Buffer"},"content":{"rendered":"<h2>Background<\/h2>\n<p>Basal Medium Eagle (BME) in Earle\u2019s Buffer originally developed by Harry Eagle. This media is one of the most widely used of all synthetic cell culture media. Furthermore, Basal Medium Eagle (BME) in Earle\u2019s Buffer, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. Moreover, PurMa Biologics manufacturers BME with Earle\u2019s as well as in Hanks&#8217; salts for use without CO<sub>2<\/sub>.<\/p>\n<h2>Ingredients<\/h2>\n<h3>PurMa\u2122 Basal Medium Eagle (BME) in Earle\u2019s Buffer contains:<\/h3>\n<h4>Earl&#8217;s buffer includes:<\/h4>\n<ol>\n<li>Calcium chloride<\/li>\n<li>Magnesium sulfate<\/li>\n<li>Potassium chloride<\/li>\n<li>Sodium bicarbonate<\/li>\n<li>Sodium chloride<\/li>\n<li>and lastly, Sodium Phosphate, monobasic<\/li>\n<\/ol>\n<p>PurMa Biologics so far, manufactures 109 types of BME in Earle\u2019s buffer.<\/p>\n<h2>Why PurMa Biologics? Why we stand behind our cell culture media.<\/h2>\n<p>Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, we utilize 99% pure amino acids. This will significantly increase the consistency among your experiments. Undoubtedly, considering the highest quality, our prices certainly are extremely reasonable.<\/p>\n<h2>Application<\/h2>\n<p>Basal Medium Eagle (BME) in Earle\u2019s Buffer\u00a0provides an excellent nutritional capacity for suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as \u00a0primary rat astrocytes.<\/p>\n<h2>Formulation<\/h2>\n<p>for complete formulation click here, alternatively you can find it in \u201cFormulation Tab\u201d:\u00a0 <a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/BME-with-Earls-Buffer-P3S310891.pdf\"><strong><span style=\"color: #0000ff;\">BME with Earle&#8217;s Buffer Formulation<\/span> <\/strong><\/a><\/p>\n<p><strong>References:<\/strong><\/p>\n<ol>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/4322279\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. <\/span><\/a>Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128\/am.21.1.1-5.1971. PMID:<\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/14464909\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. <\/span><\/a>LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128\/jb.83.3.475-482.1962. PMID: 14464909<\/li>\n<\/ol>\n<div class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\">Parameter<\/th>\n<th align=\"left\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\">Appearance<\/td>\n<td align=\"left\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">pH<\/td>\n<td align=\"left\">7.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Osmolality<\/td>\n<td align=\"left\">275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Endotoxin<\/td>\n<td align=\"left\">NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Mycoplasma<\/td>\n<td align=\"left\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Suitability<\/td>\n<td align=\"left\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Additive<\/td>\n<td align=\"left\">N\/A<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Indicator<\/td>\n<td align=\"left\">Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Mycoplasma Detection<\/td>\n<td align=\"left\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Sterility Tested<\/td>\n<td align=\"left\">Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Form<\/td>\n<td align=\"left\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Shipping Condition<\/td>\n<td align=\"left\">\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Basal Medium Eagle (BME) in Earl\u2019s Buffer developed by Harry Eagle, and certainly is one of the most widely used of all synthetic cell culture media. BME\u00a0 has demonstrated widely applicable\u00a0 for supporting monolayer cell\u00a0 growth in wide variety of normal as well as transformed cell lines.<\/p>\n","protected":false},"featured_media":127430,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,976],"product_tag":[514,519,520,682,545,683,546],"class_list":["post-113833","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-basal-medium-eagle-bme-in-earles-buffer","product_tag-bme","product_tag-earles-buffer","product_tag-ebss","product_tag-glucose","product_tag-phenol-red","product_tag-sodium-bicarbonate","product_tag-without-phenol-red","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/113833","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=113833"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127430"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=113833"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=113833"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=113833"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=113833"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}