It is absolutely crucial to mention that the\u00a0PurMa\u2122 Growth Supplement for each conventional media such as DMEM, IMDM, RPMI-1640\u2026 (base media) are slightly different in combination, amount of several growth factors, ions, chemicals, pH with particular adjustments needs to be done, based on the environments the base media provides.<\/p>\n
This provides a consistent and controlled environment for cell growth and research. Noticeably, CD-MEM- EBSS Medium is essential for applications that require reproducibility and regulatory compliance as well as for studying specific cellular processes, without extraneous variables. Furthermore, CD-MEM- EBSS Medium is designed for custom formulations tailored for various cell types<\/p>\n
<\/p>\n
![\"Graph](\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2024\/07\/Graph-of-CD-BME-in-EBSS-1080x618.jpg\")
<\/p>\n
Important Notes<\/h2>\n
It is crucially vital for a gradual transition from media containing FBS to chemically defined cell culture media, follow these steps:<\/p>\n
- \n
- Starting Point: 10% FBS+ (control) (you need two controls, one to be left in only 10% FBS and continue monitoring for comparing with 0% FBS + PurMa\u2122 <\/sup>Chemically Defined Medium. The second one is chemically defined medium + 10% FBS, which is going through the reduction of the FBS process.)<\/li>\n
- 48 hours later: 8% FBS in + PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n
- 48 hours later: 6% FBS in + PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n
- 48 hours later: 4% FBS+ PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n
- 48 hours later: 2% FBS+ PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n
- 48 hours later: 1% FBS + PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n
- 24 hours later: 0% FBS and only PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n<\/ul>\n
Importantly, for each change, spin down and fully discard the media and replace it with a lower percentage of FBS.<\/p>\n
Noticeably, you don\u2019t have to start with a huge number of cells (~500,000 cells\/ml). Additionally, we strongly recommend you start with more than one plate as some of the plates might get contaminated<\/p>\n
So, once the cells are kept in chemically defined medium, you should always keep them in the same chemically defined medium from the same vendor and the same formulation, even for freezing (see below).<\/p>\n
Usually, after you get your cells adapted to PurMa\u2122 <\/sup>Chemically Defined Medium you need to make several plates and freeze down several vials. The mentioned cells are adapted to chemically defined medium and when you thaw them in the first week, in addition to PurMa\u2122 Chemically Defined Medium, you need to add 10% PurMa\u2122 <\/sup>CD Booster (Cat# P3C10144).<\/p>\n
Formulation<\/h2>\n
The CD-MEM- EBSS Medium includes a base formulation including L-Alanine -L Glutamine: Base formulation for CD BME in EBSS P3C310891<\/strong><\/span><\/a><\/p>\n
Additionally, CD-MEM- EBSS Medium contains a proprietary synthetic cocktail of specific growth factors, hormones, and other nutrients which make it independent of any serum and any trace of animal products.<\/p>\n
References<\/h2>\n
- \n
- Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells.<\/span><\/a> Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128\/am.21.1.1-5.1971.<\/li>\n
- Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells.<\/a> <\/span>LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128\/jb.83.3.475-482.1962. PMID: 14464909<\/li>\n<\/ol>\n
\n\n\n
\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n Sterile filtered using 0.1 \u00b5m filter, 3 Times<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature, Overnight<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n <\/p>\n","protected":false},"excerpt":{"rendered":"
PurMa\u2122 Chemically Defined BME\u00a0 in Earls’ Buffer (CD-MEM- EBSS) medium does not need any serum, fully synthetic and is free of any trace of animal products.\u00a0 Additionally, the cell growth with CD-MEM- EBSS Medium is equivalent to Medium+ 10% FBS.<\/p>\n","protected":false},"featured_media":125120,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,976],"product_tag":[596,514,581,519,520,682,668,666,545,669,667,683,546],"class_list":["post-114247","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-basal-medium-eagle-bme-in-earles-buffer","product_tag-advanced","product_tag-bme","product_tag-cell-culture","product_tag-earles-buffer","product_tag-ebss","product_tag-glucose","product_tag-human-transferrin","product_tag-l-alanyl-l-glutamine","product_tag-phenol-red","product_tag-recombinant-insulin","product_tag-serum-reduction","product_tag-sodium-bicarbonate","product_tag-without-phenol-red","entry","has-media","first","instock","taxable","shipping-taxable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/114247","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=114247"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/125120"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=114247"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=114247"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=114247"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=114247"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
- Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells.<\/a> <\/span>LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128\/jb.83.3.475-482.1962. PMID: 14464909<\/li>\n<\/ol>\n
- 48 hours later: 8% FBS in + PurMa\u2122 <\/sup>Chemically Defined Medium<\/li>\n