HEPES Stock Buffer Solution is one of the commonly used buffers in cell cultures as well as biochemical analysis. HEPES maintains physiological pH despite changes in carbon dioxide concentration, therefore it acts better than bicarbonate in cell culture applications. \u00a0However, HEPES shows an unwanted photochemical process in the presence of riboflavin when exposed to ambient light to produce hydrogen peroxide. We therefore recommend keeping the mentioned solutions in the dark.<\/p>\n
PurMa Biologics manufactures Regular grade of buffer by using:\u00a0 PurMa\u2122 Cell Culture Suited Water (Cat# P3W110101)<\/a><\/p>\n We sterilize the buffers by autoclaving the solution. Finally we pack the buffer in our Class 100 Aseptic Facility.<\/p>\n PurMa Biologics manufactures this grade of buffer by using :PurMa\u2122 <\/a>Cell Culture Suited Water (Cat# P3W110101)<\/a><\/p>\n After adjusting the pH, we finally, sterilize the buffer by\u00a0 3 times passing through 0.2 \u00b5m filters. Additionally,\u00a0 We pack the buffer under N2 gas\u00a0 in our Class 100 Aseptic Facility.<\/p>\n PurMa Biologics manufactures this grade of buffer by using :PurMa\u2122 Ultra-Pure Cell Culture Suited Water (Cat# P3W110102) <\/a><\/p>\n We sterilize the buffers by autoclaving the solution.\u00a0 Additionally, we pass the buffer\u00a0 three times through 0.1 \u00b5m filters,\u00a0 and finally\u00a0 pack it under N2 gas in our Class 100 Aseptic Facility.<\/p>\n PurMa Biologics manufactures this grade of buffer by using: PurMa\u2122 Ultra-Pure Cell Culture Suited Water (Cat# P3W110102) <\/a><\/p>\n After depleting\u00a0 the endotoxins (see below for more details), we sterilize the buffer by 3 times passing through\u00a0 0.1 \u00b5m filters and pack under N2 gas in our Class 100 Aseptic Facility<\/p>\n PurMa Biologics has the most sophisticated endotoxin removal procedure. To remove this pathogen, we pass the buffer \u00a0through the PurMa\u2122 Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column,\u00a0 we covalently conjugate Sepharose 6B\u00a0 to an endotoxin-specific absorbent. We finally\u00a0 eliminate the endotoxin residual by passing the unbound fraction three times through 0.1\u00b5m filters.<\/p>\n <\/p>\nCell Culture Suited Grade Buffer<\/strong><\/h3>\n
RNase and DNase Free Grade Buffer<\/strong><\/h3>\n
Cell Culture Suited \/ Endotoxin Depleted Grade Buffer<\/strong><\/h3>\n
The Endotoxin Removing Procedure from Buffer<\/h2>\n
References<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 6.5 \u00b1 0.1; 7.0 \u00b1 0.1; 7.5 \u00b1 0.1; 7.6 \u00b1 0.1; 8.0 \u00b1 0.1; 8.5 \u00b1 0.1; 9.0 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0N\/A<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0N\/A<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n