PurMa\u2122 Trypsin-EDTA 2.5% contains ultra-pure trypsin. Furthermore, trypsin is one of the members of the serine protease family which goes through a set of intracellular signaling that results in the activation of this enzyme in the pancreas. Cell culture laboratories widely use trypsin to dissociate the adherent cells for routine cell culture. The Trypsin family is conservative. Trypsin performs the best at 37\u00b0C and at the PH 7.3-8.5.<\/p>\n
Trypsin cleaves its substrates: IGDKLIPA unless these amino acids are followed by proline. PurMa\u2122 Trypsin is HPLC purified 99% purity for bovine pancreas. Moreover, optical rotatory dispersion of trypsin in the presence of calcium shows a major conformational transition between 40 and 45\u00b0 which results in deactivation of trypsin. Therefore, all the buffers needed to be Ca+2 & Mg+2\u00a0free for the proper function of this enzyme.<\/p>\n
Trypsin treatment abolishes the ability of mycoplasmas to attach to the cells. Mycoplasma is one of the common and slowly deteriorating contaminations in cell culture, especially due to the invisibility of the contamination. PurMa Biologics manufactures all the reagents you need to fight mycoplasma in your cell culture. The presence of a trace of SDS and other detergents leads to a decrease in the trypsin activity.<\/p>\n
The activity of this enzyme is further modulated by the sulfation of Tyr154. To overcome the mentioned issue, we use our most sophisticated water quality to ensure the absence of Mg+2 and Ca+2 as well as other heavy metals, please click here: PurMa\u2122 Ultra-Pure Cell Culture Suited Water (Cat# P3W110102) <\/a><\/p>\n Additionally, we include 2mM EDTA as it chelates metals such as calcium, magnesium, lead, mercury, copper, iron\u2026<\/p>\n PurMa Biologics manufactures 0.05%, 0.25%,0.5%, 2.5%\u00a0and 5% Trypsin with EDTA as well as without EDTA in phenol red or without phenol red. Moreover, PurMa\u2122 Trypsin is made in Hanks\u2019 Buffer with 2mM EDTA (pH 7.8).<\/p>\n The complete formulation can be found here: Trypsin with EDTA. 2.5% Formulation<\/a><\/strong><\/span><\/p>\n <\/p>\nFeatures and Benefits<\/h2>\n
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Different Concentrations of Trypsin<\/h2>\n
Formulation<\/h2>\n
References<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.8 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0EDTA<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n