PurMa\u2122 Trypsin EDTA Free 0.05% is a trypsin without EDTA, an ultra-pure, low-strength solution for gentle dissociation and subculturing of sensitive or delicate adherent cells in mammalian cell culture. Trypsin is a serine protease that cleaves peptide bonds at lysine and arginine residues (except when followed by proline), releasing cells from the culture surface and from one another for passaging, counting, and downstream work.<\/p>\n
Many trypsin solutions include EDTA, a chelating agent that binds calcium and magnesium ions to speed up cell detachment. PurMa\u2122 Trypsin EDTA Free 0.05% contains no EDTA, giving you the gentlest, most controlled dissociation in the range. At this low concentration, it is well suited to delicate cell lines and to protocols where you want to preserve divalent-cation-dependent surface proteins and functions.<\/p>\n
PurMa Biologics produces trypsin in 0.05%, 0.25%, 0.5%, 2.5%, and 5%, both with EDTA and EDTA-free, in phenol red or phenol-red-free formats, so you can match the strength and indicator to your protocol.<\/p>\n
| Parameter<\/th>\n | Specification<\/th>\n<\/tr>\n<\/thead>\n |
|---|---|
| Appearance<\/td>\n | Red, clear liquid<\/td>\n<\/tr>\n |
| pH<\/td>\n | 7.8 \u00b1 0.1<\/td>\n<\/tr>\n |
| Osmolality<\/td>\n | 275\u2013360 mOsm\/L<\/td>\n<\/tr>\n |
| Endotoxin<\/td>\n | NMT < 2 EU\/mL<\/td>\n<\/tr>\n |
| Mycoplasma<\/td>\n | Negative<\/td>\n<\/tr>\n |
| Sterility<\/td>\n | Sterile filtered (0.22 \u00b5m)<\/td>\n<\/tr>\n |
| Indicator<\/td>\n | Phenol red<\/td>\n<\/tr>\n |
| Form<\/td>\n | Liquid<\/td>\n<\/tr>\n |
| Suitability<\/td>\n | Mammalian cell culture<\/td>\n<\/tr>\n |
| Shipping<\/td>\n | Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\nReferences<\/h2>\n<\/p>\n |