PurMa\u2122 Trypsin EDTA Free 0.5% is a\u00a0trypsin without EDTA<\/strong>, an ultra-pure solution used to gently dissociate adherent cells in mammalian cell culture. Trypsin is a serine protease that cleaves peptide bonds at lysine and arginine residues (except when followed by proline), releasing cells from the culture surface and from one another for passaging, counting, and downstream work.<\/p>\n Many trypsin solutions include EDTA, a chelating agent that binds calcium and magnesium ions to speed up detachment. PurMa\u2122 Trypsin EDTA Free 0.5% contains\u00a0no EDTA<\/strong>, giving you a gentler, more controlled dissociation. It’s preferred when working with cells sensitive to EDTA or to calcium\/magnesium depletion, or when you want to preserve divalent-cation-dependent surface proteins and functions.<\/p>\n PurMa Biologics produces trypsin in 0.05%, 0.25%, 0.5%, 2.5%, and 5%, both with EDTA and EDTA-free, in phenol red or phenol-red-free formats, so you can match the strength and indicator to your protocol.<\/p>\nWhy Trypsin Without EDTA?<\/h2>\n
Features and Benefits<\/h2>\n
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Available Concentrations<\/h2>\n
Specifications<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n 7.8 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275\u2013360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT < 2 EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility<\/td>\n Sterile filtered (0.22 \u00b5m)<\/td>\n<\/tr>\n \n Indicator<\/td>\n Phenol red<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Suitability<\/td>\n Mammalian cell culture<\/td>\n<\/tr>\n \n Shipping<\/td>\n Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n References<\/h2>\n
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