PurMa\u2122 Trypsin EDTA Free 5% contains ultra-pure trypsin. This enzyme is a member of the serine protease family. Trypsin, initially produced in the pancreas, is subsequently secreted and activated in the intestines. Cell culture laboratories use trypsin to dissociate adherent cells for routine cell culture.<\/p>\n
Trypsin cleaves its substrates: IGDKLIPA unless these amino acids are followed by proline. PurMa\u2122 trypsin is HPLC purified at 99% purity for bovine pancreas. Moreover, optical rotatory dispersion of trypsin in the presence of calcium shows a major conformational transition between 40 and 45\u00b0 which results in deactivation of trypsin. Therefore, all the buffers needed to be Ca+2 & Mg+2 free for the proper function of this enzyme.<\/p>\n
The activity of this enzyme is further modulated by the sulfation of Tyr154. To overcome the mentioned issue, we use our most sophisticated water quality to ensure the absence of Mg+2 and Ca+2 as well as other heavy metals, please see: PurMa\u2122 Ultra-Pure Cell Culture Suited Water (Cat# P3W110102) <\/a><\/span><\/p>\n Additionally, we include 2mM EDTA as it chelates metals such as calcium, magnesium, lead, mercury, copper, iron\u2026<\/p>\n Trypsin treatment abolishes the ability of mycoplasmas to attach to the cells. Incidentally, mycoplasma is a common, slowly deteriorating contamination in cell culture, especially due to the invisibility of the contamination. PurMa Biologics manufactures all the reagents you need to fight mycoplasma in your cell culture. The presence of a trace of SDS and other detergents leads to a decrease in the trypsin activity.<\/p>\n PurMa Biologics manufactures trypsin without EDTA 0.05 % as a complete Ready-To-Use solution that contains 1X Hanks\u2019 buffer and DOES NOT need any additional ingredients.<\/p>\n PurMa Biologics produces 0.05%, 0.25%, 0.5%, 2.5%, and 5% Trypsin with EDTA as well as without EDTA in phenol red or without phenol red. Moreover, we manufacture PurMa\u2122 Trypsin in Hanks\u2019 Buffer with 2mM EDTA (pH 7.8).<\/p>\n Here is the complete formulation: 5% Trypsin without EDTA\u00a0<\/a><\/strong><\/span><\/p>\n Adhesion of mycoplasmas to eukaryotic cells. <\/a><\/span>Razin et al. Ciba Found Symp. 1981;80:98-118. doi: 10.1002\/9780470720639.ch8. PMID: 6790254.<\/p>\n Fate of trypsin and chymotrypsin in the human small intestine. <\/a><\/span>Goldberg DM, Roy AD. 1969 Jun;10(6):477-83. doi: 10.1136\/gut.10.6.477. PMID: 5786145.<\/p>\nPurMa\u2122 Trypsin Deactivates Mycoplasma in your Cell Culture<\/h2>\n
Features and Benefits<\/h2>\n
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Concentrations<\/h2>\n
Formulation<\/h2>\n
References<\/h2>\n