{"id":57733,"date":"2023-06-28T23:00:57","date_gmt":"2023-06-28T23:00:57","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=57733"},"modified":"2026-07-31T15:47:40","modified_gmt":"2026-07-31T21:47:40","slug":"purmafectin-ultra-transfection-reagent","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/purmafectin-ultra-transfection-reagent\/","title":{"rendered":"PurMaFectin\u2122 Ultra Transfection Reagent"},"content":{"rendered":"<h2>Background<\/h2>\n<p>PurMa Biologics manufactures PurMaFectin\u2122 Ultra Transfection Reagent with over 97% efficacy. Also, this reagent performs in a non-liposomal manner. Our Ultra Transfection Reagent provides consistent\/reproducible transfection with ignorable cytotoxicity. PurMaFectin\u2122 Ultra combines with DNA, protects it from lysosomal degradation and facilitates efficient plasmid delivery to mammalian\/ eukaryotic nucleus.<\/p>\n<h2>Special Features<\/h2>\n<p>For complete protocol, click here: <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/PurMaFectin_Ultra_UserMannual.pdf\"><strong>PurMaFectin\u2122 Ultra_ Use Manual<\/strong><\/a><\/span><\/p>\n<ul>\n<li>\n<ul>\n<li>Basically, the entire procedure with PurMaFectin\u2122 Ultra is completed within 30 minutes.<\/li>\n<\/ul>\n<ul>\n<li>Furthermore, for the highest efficacy and the lowest dead cells, transfect cells at around 70~80% confluency. We strongly recommend starting the seeding by counting the trypsinized cells so there will be high consistency among your experiments.<\/li>\n<li>Additionally, different cell types react differently to the number of passages when they are used for transfection. So, to minimize the impact of passage on efficiency of transfection, use the cells with the same passage number, or alternatively, apply at least two different concentrations of transfection reagent as control in new transfect\u00a0experiments before the condition is optimized.<\/li>\n<li>We deplete endotoxin from our transfection reagent. The\u00a0 endotoxin-contaminated DNA results in inefficient transfection and can cause high cellular toxicity. To deplete the endotoxin, we use <strong>PurMa<sup>TM <\/sup>Endotoxin Elimination Column (Cat# P6H1216251). <\/strong>In this proprietary kit, Sepharose 6B covalently conjugates to\u00a0 an endotoxin-specific absorbent. The mentioned procedure will end with passing the reagent through 0.1\u00b5m filters 3X (included in the kit). The mentioned procedure eliminates the residual endotoxin.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<table width=\"460\">\n<tbody>\n<tr>\n<td width=\"201\"><strong>Parameter<\/strong><\/td>\n<td width=\"259\"><strong>Specification<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Appearance<\/td>\n<td width=\"259\">clear liquid, Red liquid<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">pH<\/td>\n<td width=\"259\">7.4 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Osmolality<\/td>\n<td width=\"259\">275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Endotoxin<\/td>\n<td width=\"259\">NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Mycoplasma<\/td>\n<td width=\"259\">Negative<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Suitability<\/td>\n<td width=\"259\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Additive<\/td>\n<td width=\"259\">Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Indicator<\/td>\n<td width=\"259\">NA<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Sterility Tested<\/td>\n<td width=\"259\">Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Form<\/td>\n<td width=\"259\">Liquid<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Shipping Condition<\/td>\n<td width=\"259\">Dry Ice<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>References<\/h2>\n<ol>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30686003\/\" rel=\"nofollow noopener\" target=\"_blank\">Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents. <\/a>Rahimi et al. 2018;119(11):701-705. doi: 10.4149\/BLL_2018_125. PMID: 30686003.<\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/8922638\/\" rel=\"nofollow noopener\" target=\"_blank\">Transfection maximizer increases the efficiency of calcium phosphate transfections with mammalian cells. <\/a>Zhang et al. Biotechniques. 1996 Nov;21(5):940-2, 944-5. doi: 10.2144\/96215pf02. PMID: 8922638.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PurMa Biologics manufactures PurMaFectin\u2122 Ultra Transfection Reagent wth over 97% efficacy. Also, this reagent performs as non-liposomal. Our Ultra Transfection Reagent provides consistent\/reproducible transfection with ignorable cytotoxicity PurMaFectin\u2122 Ultra combines with DNA, protects it from lysosomal degradation and facilitates efficient plasmid delivery to mammalian\/ eukaryotic nucleus.<\/p>\n","protected":false},"featured_media":127251,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[30],"product_tag":[636,646,638,571,633,634,639,640],"class_list":["post-57733","product","type-product","status-publish","has-post-thumbnail","product_cat-transfection-reagents","product_tag-antibiotic-mix-solution","product_tag-bacteria","product_tag-mold","product_tag-mycoplasma","product_tag-penicillin","product_tag-streptomycin","product_tag-virus","product_tag-yeast","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/57733","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=57733"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127251"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=57733"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=57733"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=57733"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=57733"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}