{"id":58363,"date":"2023-07-06T04:40:33","date_gmt":"2023-07-06T04:40:33","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=58363"},"modified":"2026-08-13T13:47:17","modified_gmt":"2026-08-13T19:47:17","slug":"bovine-calf-serum-bcs","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/bovine-calf-serum-bcs\/","title":{"rendered":"Bovine Calf Serum (BCS)"},"content":{"rendered":"<h2>Background<\/h2>\n<p>Laboratories around the globe use Bovine Calf Serum (BCS)\u00a0 to support the growth of many cell types <em>in vitro<\/em>. BCS provides proper growth for human Embryonic Fibroblast as well as models of hepatitis. Moreover, it performs well with U937 macrophage, human lymphocytes as well as blood cells including cord blood cells, T and B lymphocytes. We collect <sup>\u00a0<\/sup>Bovine Calf Serum (BCS) from calves less than 12 months old.<\/p>\n<p>Not sure which serum fits your cells? See our complete guide to\u00a0<a href=\"\/cell-culture-serum\/\">cell culture serum.<\/a><\/p>\n<h3>Bovine Calf Serum (BCS) Vs. Fetal Bovine Serums (FBS)<\/h3>\n<ol>\n<li>BCS contains approximately two-fold the protein compared to fetal bovine serum (FBS); among them are IgGs and other antibodies.<\/li>\n<li>Contains less growth factors than fetal bovine serum, however, it is proven to be adequate for the above-mentioned cell lines.<\/li>\n<li>Importantly, it is much more cost-effective than Fetal Bovine Serum (FBS).<\/li>\n<\/ol>\n<h3>Special Features<\/h3>\n<ul>\n<li>PurMa<sup>TM<\/sup> Bovine Calf Serum (BCS) has US\u00a0 origin.<\/li>\n<li>PurMa <sup>TM <\/sup>Bovine Calf Serum (BCS) has been triple-filtered through 0.1 \u00b5M filters.<\/li>\n<li>\u00a0PurMa Biologics sterilizes BCS with\u00a0 gamma irradiation methodology.<\/li>\n<li>PurMa Biologics\u00a0 performs heat-inactivation on our BCS.<\/li>\n<li>Has been tested for mycoplasma as well as endotoxin (see below), see the certificate of analysis.<\/li>\n<li>Has been tested on multiple cell lines, such as Hek293, COS1, and NS1 cells.<\/li>\n<\/ul>\n<h3>Analysis<\/h3>\n<p>For full\u00a0 Analysis\u00a0 performed on all PurMa Bovine and Horse Serums click here, as well as in &#8220;Documents&#8221; Tab: <strong><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Routin-Patalogicsl-Analysis-on-PurMa-Bovine-and-Horse-Serums.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"color: #0000ff;\">Routine Pathological Analysis on PurMa Bovine and Horse Serums<\/span><\/a><\/strong><\/p>\n<h3>References<\/h3>\n<ol>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/311308\/\" rel=\"nofollow noopener\" target=\"_blank\">Mitogenicity of fetal calf serum. <\/a><\/span>Gergely et al. 1977;11(3-4):259-63. PMID: 311308<\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31936491\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Fetal Calf Serum Exerts an Inhibitory Effect on Replication of Duck Hepatitis A Virus Genotype 1 in Duck Embryo Fibroblast Cells<\/span>. <\/a>Wang, et al. Viruses. 2020 Jan 9;12(1):80. doi: 10.3390\/v12010080. PMID: 31936491.<\/li>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33467719\/\" rel=\"nofollow noopener\" target=\"_blank\">Eicosanoid Content in Fetal Calf Serum Accounts for Reproducibility Challenges in Cell Culture. <\/a><\/span>Niederstaetter et al. 2021 Jan 15;11(1):113. doi: 10.3390\/biom11010113. PMID: 33467719.<\/li>\n<\/ol>\n<table width=\"708\">\n<tbody>\n<tr>\n<td width=\"366\"><strong>Methodology<\/strong><\/td>\n<td width=\"120\"><strong>Unit measured<\/strong><\/td>\n<td width=\"108\"><strong>Accepted level<\/strong><\/td>\n<td width=\"114\"><strong>Result<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Sterility (Current USP and EP 2.6.1 for Bacteria &amp; Fungi)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">pH<\/td>\n<td width=\"120\">N\/A<\/td>\n<td width=\"108\">measured<\/td>\n<td width=\"114\">6.58<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Osmolality (USP 785)\u00a0\u00a0\u00a0\u00a0 mOsm\/KgH2<\/td>\n<td width=\"120\">mOsm\/KgH20<\/td>\n<td width=\"108\">280-340<\/td>\n<td width=\"114\">288<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Hemoglobin (Fleming &amp; Woolf)<\/td>\n<td width=\"120\">mg\/dL<\/td>\n<td width=\"108\">&lt;25<\/td>\n<td width=\"114\">12.9<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Endotoxin (USP 85)<\/td>\n<td width=\"120\">EU\/mL<\/td>\n<td width=\"108\">&lt;20<\/td>\n<td width=\"114\">&lt;0.100<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Mycoplasma (Barile &amp; Kern; Large Volume, Direct Culture)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">IgG<\/td>\n<td width=\"120\">Elliza<\/td>\n<td width=\"108\">Measured<\/td>\n<td width=\"114\">0.19 ug\/ml<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Albumin<\/td>\n<td width=\"120\">Eliza<\/td>\n<td width=\"108\">Measured<\/td>\n<td width=\"114\">\u2264 4.6 g\/dL<\/td>\n<\/tr>\n<tr>\n<td width=\"366\"><strong>Virus Testing (9 CFR 113.53c)<\/strong><\/td>\n<td width=\"120\"><\/td>\n<td width=\"108\"><\/td>\n<td width=\"114\"><\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Adenovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Parvovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Respiratory Syncytial Virus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Viral Diarrhea Virus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Rabies<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Reovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Cytopathogenic Agents (IBR)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Hemadsorbing Agents (PI3)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bluetongue<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-bovine-calf-serum\/\">What Is Bovine Calf Serum?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A cost-effective growth supplement used in labs worldwide for routine in vitro culture of many cell types.<\/p>\n<ul>\n<li>Supports growth of human embryo fibroblasts and hepatitis models<\/li>\n<li>Collected from calves less than 12 months old<\/li>\n<li>US-origin serum for general cell and tissue culture<\/li>\n<\/ul>\n","protected":false},"featured_media":82202,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[31,589],"product_tag":[649,650],"class_list":["post-58363","product","type-product","status-publish","has-post-thumbnail","product_cat-fetal-bovine-serum","product_cat-bovine-serums","product_tag-bovine-serum","product_tag-calf-serum","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/58363","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=58363"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/82202"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=58363"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=58363"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=58363"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=58363"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}