{"id":58401,"date":"2023-07-08T01:37:00","date_gmt":"2023-07-08T01:37:00","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=58401"},"modified":"2026-07-31T14:00:53","modified_gmt":"2026-07-31T20:00:53","slug":"endotoxin-depleted-fetal-bovine-serum","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/endotoxin-depleted-fetal-bovine-serum\/","title":{"rendered":"Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)"},"content":{"rendered":"<h3>Background<\/h3>\n<p>PurMa Biologics manufactures seven types of bovine serum, three of which, however, are considered premium fetal bovine serums, each specialized for certain requirements in tissue culture. Moreover,\u00a0 We highly evaluate Endotoxin-Depleted Fetal Bovine Serum (ED-FBS) for endotoxin and after triple filtering with 0.1\u00b5m, \u00a0the residual endotoxin was depleted using PurMa<sup>TM <\/sup>Endotoxin Elimination Column (Cat# P6H1216251) ( see below).<\/p>\n<p>Not sure which serum fits your cells? See our complete guide to <a href=\"\/cell-culture-serum\/\">cell culture serum.<\/a><\/p>\n<h2>PurMa<sup>TM <\/sup>Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)<\/h2>\n<ul>\n<li>PurMa<sup>TM <\/sup>Endotoxin Depleted Fetal Bovine Serum (EDP-FBS) is of USA origin.<\/li>\n<li>Additionally, has gone through\u00a0 an endotoxin depletion procedure.<\/li>\n<li>Has gone through\u00a0 gamma irradiation as well as heat inactivation process.<\/li>\n<li>Lastly, we vigorously analyze various aspects and each LOT possesses a certificate of analysis.<\/li>\n<\/ul>\n<h3>Endotoxin Removal Procedure<\/h3>\n<ol>\n<li>PurMa Biologics processes PurMa<sup>TM<\/sup> ED-FBS from PurMa<sup>TM<\/sup> Premium Fetal Bovine Serum (P-FBS).\u00a0\u00a0It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.<\/li>\n<li>The process of removing endotoxin from FBS\u00a0 routinely starts with passing the premium FBS through the PurMa<sup>TM <\/sup>Endotoxin Elimination Column (Cat# P6H1216251) at the rate of 0.1 ml\/minute.<\/li>\n<li>The unbound fractions pass through 0.1\u00b5m filters three times. The mentioned procedure eliminates the residual of endotoxin as well.<\/li>\n<li>We conjugate Sepharose 6B in our proprietary column covalently to a powerful endotoxin-specific absorbent.<\/li>\n<\/ol>\n<h3>Analysis<\/h3>\n<p>PurMa biologics routinely performs pathological analysis on PurMa bovine and horse serums:<span style=\"color: #0000ff;\">\u00a0<strong><a style=\"color: #0000ff;\" href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Routin-Patalogicsl-Analysis-on-PurMa-Bovine-and-Horse-Serums.pdf\" target=\"_blank\" rel=\"noopener\">Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums<\/a><\/strong><\/span><\/p>\n<h3>References<\/h3>\n<ol>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/2805682\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Biochemical and morphological studies of steady state and lipopolysaccharide treated bovine articular cartilage explant cultures<\/span>. <\/a>Tian et al. Connect Tissue Res. 1989;19(2-4):195-218. doi: 10.3109\/03008208909043897. PMID:<\/li>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/7421602\/\" rel=\"nofollow noopener\" target=\"_blank\">The preparation of antigenic hapten-carrier conjugates: a survey. <\/a><\/span>Erlanger BF. Methods Enzymol. 1980;70(A):85-104. doi: 10.1016\/s0076-6879(80)70043-5.<\/li>\n<\/ol>\n<table width=\"708\">\n<tbody>\n<tr>\n<td width=\"366\"><strong>Methodology<\/strong><\/td>\n<td width=\"120\"><strong>Unit measured<\/strong><\/td>\n<td width=\"108\"><strong>Accepted level<\/strong><\/td>\n<td width=\"114\"><strong>Result<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Sterility (Current USP and EP 2.6.1 for Bacteria &amp; Fungi)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">pH<\/td>\n<td width=\"120\">N\/A<\/td>\n<td width=\"108\">measured<\/td>\n<td width=\"114\">6.58<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Osmolality (USP 785)\u00a0\u00a0\u00a0\u00a0 mOsm\/KgH2<\/td>\n<td width=\"120\">mOsm\/KgH20<\/td>\n<td width=\"108\">280-340<\/td>\n<td width=\"114\">288<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Hemoglobin (Fleming &amp; Woolf)<\/td>\n<td width=\"120\">mg\/dL<\/td>\n<td width=\"108\">&lt;25<\/td>\n<td width=\"114\">12.9<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Endotoxin (USP 85)<\/td>\n<td width=\"120\">EU\/mL<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Mycoplasma (Barile &amp; Kern; Large Volume, Direct Culture)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">IgG<\/td>\n<td width=\"120\">Elliza<\/td>\n<td width=\"108\">Measured<\/td>\n<td width=\"114\">0.19 ug\/ml<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Albumin<\/td>\n<td width=\"120\">Eliza<\/td>\n<td width=\"108\">Measured<\/td>\n<td width=\"114\">\u2264 4.6 g\/dL<\/td>\n<\/tr>\n<tr>\n<td width=\"366\"><strong>Virus Testing (9 CFR 113.53c)<\/strong><\/td>\n<td width=\"120\"><\/td>\n<td width=\"108\"><\/td>\n<td width=\"114\"><\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Adenovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Parvovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Respiratory Syncytial Virus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Viral Diarrhea Virus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Rabies<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Reovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Cytopathogenic Agents (IBR)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Hemadsorbing Agents (PI3)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bluetongue<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-fetal-bovine-serum-used-for\/\">What Is Fetal Bovine Serum Used For?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fetal bovine serum with endotoxin reduced to very low levels for endotoxin-sensitive work.<\/p>\n<ul>\n<li>Triple filtered at 0.1 &micro;m, then processed through PurMa&#8217;s Endotoxin Elimination Column<\/li>\n<li>Each lot evaluated for endotoxin<\/li>\n<li>Ideal where residual endotoxin can interfere with results<\/li>\n<\/ul>\n","protected":false},"featured_media":127220,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[31,589],"product_tag":[648,647],"class_list":["post-58401","product","type-product","status-publish","has-post-thumbnail","product_cat-fetal-bovine-serum","product_cat-bovine-serums","product_tag-endotoxin-depleted","product_tag-fetal-bovine-serum","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/58401","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=58401"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127220"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=58401"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=58401"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=58401"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=58401"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}