{"id":58407,"date":"2023-07-08T02:50:04","date_gmt":"2023-07-08T02:50:04","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=58407"},"modified":"2026-08-18T14:40:01","modified_gmt":"2026-08-18T20:40:01","slug":"lipid-depleted-fetal-bovine-serum-ld-fbs","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/lipid-depleted-fetal-bovine-serum-ld-fbs\/","title":{"rendered":"Lipid-Depleted Fetal Bovine Serum (LD-FBS)"},"content":{"rendered":"<h2>Background<\/h2>\n<p>The analysis of lipid metabolism in cultured cells is challenging. That is because the cell culture is carried out in the presence of animal serum, which contains a wide range of undefined lipid species. Generally, the best uses for Lipid-Depleted Fetal Bovine Serum (LD-FBS, also called delipidated FBS) in research applications are:<\/p>\n<ol>\n<li>When the normal concentration of lipids may interfere with the results.<\/li>\n<li>For studies that directly involve lipid metabolism. That is because the composition of serum can have a profound impact on important cellular checkpoints.<\/li>\n<li>It is used for the generation of media with defined lipid species.<\/li>\n<\/ol>\n<p>Not sure which serum fits your cells? See our complete guide to<a href=\"\/cell-culture-serum\/\"> cell culture serum<\/a>.<\/p>\n<h2>Special Features<\/h2>\n<ul>\n<li>PurMa<sup>TM <\/sup>Premium LD-FBS is of USA origin<\/li>\n<li>Additionally, has gone through\u00a0 an endotoxin depletion procedure.<\/li>\n<li>Has gone through gamma irradiation as well as heat inactivation process.<\/li>\n<li>PurMa Biologics performs the most sophisticated lipid depletion.<\/li>\n<li>The process will continue with dialysis using the 3500 Dalton dialysis tube.<\/li>\n<li>The dialysis carries out against 5X 10 Liters of PBD with physiological pH per each 500 ml of serum.<\/li>\n<li>The procedure depletes the lipids, phospholipids, and a long chain of fatty acids.<\/li>\n<li>Lastly, we vigorously analyze various aspects, and each LOT possesses a certificate of analysis.<\/li>\n<li>The process eliminates mycoplasma, IgG, steroids and hormones, and other pathogens.<\/li>\n<li>Acceptable level of hemoglobin (20 mg\/DL) and endotoxin (10 mg\/DL).<\/li>\n<\/ul>\n<h2>Analysis<\/h2>\n<p>An example of results following PurMa\u2122 FBS Lipid depletion procedure : <span style=\"color: #0000ff;\"><strong><a style=\"color: #0000ff;\" href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/An-Example-of-Analysis-on-PurMa-LD-FBS.pdf\" target=\"_blank\" rel=\"noopener\">The Lipid Analysis of Lot: 30143050032423 following Lipid Depletion<\/a><\/strong><\/span><\/p>\n<p>For non-proprietary information in PurMa biologics\u2019 general procedure for FBS Lipid depletion, click here:\u00a0 <a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/FBS-Lipid-Deplition-procedure.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"color: #0000ff;\"><strong>PurMa\u2122 FBS Lipid depletion Procedure<\/strong><\/span><\/a><\/p>\n<p>Routine pathological analysis on PurMa bovine and horse serums:\u00a0<strong><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Routin-Patalogicsl-Analysis-on-PurMa-Bovine-and-Horse-Serums.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"color: #0000ff;\">Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums<\/span><\/a><\/strong><\/p>\n<h2>References<\/h2>\n<ol>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2135985\/\" rel=\"nofollow noopener\" target=\"_blank\">Effect of the removal of lipids on specific precipitation<\/a>.<\/span> Krueger et al. J Exp Med. 1950 Oct 31; 92(5): 383\u2013391. doi:\u00a010.1084\/jem.92.5.383. PMCID: PMC2135985.<\/li>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8275250\/\" rel=\"nofollow noopener\" target=\"_blank\">Preparation of Lipid-Stripped Serum for the Study of Lipid Metabolism in Cell Culture<\/a>.<\/span> Aaron et al. 2018 Jun 5; 8(11): e2876. Published online 2018 Jun 5.doi:10.21769\/Bio Pro toc.2876, PMCID: PMC8275250.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<table width=\"692\">\n<tbody>\n<tr>\n<td width=\"196\"><strong>Methodology<\/strong><\/td>\n<td width=\"165\"><strong>Unit measured<\/strong><\/td>\n<td width=\"165\"><strong>Accepted level<\/strong><\/td>\n<td width=\"165\"><strong>Result<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Sterility (Current USP and EP 2.6.1 for Bacteria &amp; Fungi)<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">pH<\/td>\n<td width=\"165\">N\/A<\/td>\n<td width=\"165\">measured<\/td>\n<td width=\"165\">6.58<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Osmolality (USP 785)\u00a0\u00a0\u00a0\u00a0 mOsm\/KgH2<\/td>\n<td width=\"165\">mOsm\/KgH20<\/td>\n<td width=\"165\">280-340<\/td>\n<td width=\"165\">288<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Hemoglobin (Fleming &amp; Woolf)<\/td>\n<td width=\"165\">mg\/dL<\/td>\n<td width=\"165\">&lt;25<\/td>\n<td width=\"165\">12.9<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Endotoxin (USP 85)<\/td>\n<td width=\"165\">EU\/mL<\/td>\n<td width=\"165\">&lt;20<\/td>\n<td width=\"165\">&lt;0.100<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Mycoplasma (Barile &amp; Kern; Large Volume, Direct Culture)<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">IgG<\/td>\n<td width=\"165\">Elliza<\/td>\n<td width=\"165\">Measured<\/td>\n<td width=\"165\">0.19 ug\/ml<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Albumin<\/td>\n<td width=\"165\">Eliza<\/td>\n<td width=\"165\">Measured<\/td>\n<td width=\"165\">\u2264 4.6 g\/dL<\/td>\n<\/tr>\n<tr>\n<td width=\"196\"><strong>Virus Testing (9 CFR 113.53c)<\/strong><\/td>\n<td width=\"165\"><\/td>\n<td width=\"165\"><\/td>\n<td width=\"165\"><\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Bovine Adenovirus<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Bovine Parvovirus<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Bovine Respiratory Syncytial Virus<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Bovine Viral Diarrhea Virus<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Rabies<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Reovirus<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Cytopathogenic Agents (IBR)<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Hemadsorbing Agents (PI3)<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"196\">Bluetongue<\/td>\n<td width=\"165\">NA<\/td>\n<td width=\"165\">Not Detected<\/td>\n<td width=\"165\">Not Detected<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-fetal-bovine-serum-used-for\/\">What Is Fetal Bovine Serum Used For?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fetal bovine serum with lipids removed, for studies where serum lipids would confound results.<\/p>\n<ul>\n<li>Best for lipid-metabolism research and lipid-sensitive assays<\/li>\n<li>Removes lipids that can affect key cellular checkpoints<\/li>\n<li>Enables preparation of media with defined lipid species<\/li>\n<\/ul>\n","protected":false},"featured_media":127219,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[31,589],"product_tag":[647,642],"class_list":["post-58407","product","type-product","status-publish","has-post-thumbnail","product_cat-fetal-bovine-serum","product_cat-bovine-serums","product_tag-fetal-bovine-serum","product_tag-lipid-depleted","entry","has-media","first","instock","taxable","shipping-taxable","purchasable","product-type-variable","has-rating","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/58407","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=58407"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127219"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=58407"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=58407"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=58407"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=58407"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}