{"id":58414,"date":"2023-07-09T01:05:29","date_gmt":"2023-07-09T01:05:29","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=58414"},"modified":"2026-08-13T13:47:17","modified_gmt":"2026-08-13T19:47:17","slug":"dialyzed-fetal-bovine-serum-d-fbs","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/dialyzed-fetal-bovine-serum-d-fbs\/","title":{"rendered":"Dialyzed Fetal Bovine Serum (D-FBS)"},"content":{"rendered":"<h3>Background<\/h3>\n<p>Dialyzed Fetal Bovine Serum (D-FBS) provides more consistency among the cell culture experiments. That especially stands out in studies requiring a more defined environment of small molecules. Moreover, the dialysis process eliminates\/reduces the concentration of low molecular weight components. That includes antibiotics, nucleotides, amino acids, hormones, salts, as well as various small proteins in the serum.<\/p>\n<p><strong>PurMa<sup>TM<\/sup> Dialyzed FBS is Free of Tetracycline and other Antibiotics.<\/strong><\/p>\n<p>Not sure which serum fits your cells? See our complete guide to <a href=\"\/cell-culture-serum\/\">cell culture serum.<\/a><\/p>\n<h2>PurMa<sup>TM<\/sup> Dialyzed FBS (DFBS)<\/h2>\n<ul>\n<li>PurMa<sup>TM<\/sup> Premium FBS is of USA origin.<\/li>\n<li>We perform the dialysis using the 3500 Dalton (3.5 kDa) dialysis tube. The procedure carries out against 10X 10-liter PBS (pH 7.4). At the end, the dialyzed FBS is sterilized by 0.1 \u00b5M filters three times.<\/li>\n<li>The parent FBS has gone through gamma irradiation as well as heat inactivation process.<\/li>\n<li>PurMa Biologics performs vigorous analysis following the dialysis, which results in a certificate of analysis.<\/li>\n<li>Free of Mycoplasma, steroids and hormones, and other pathogens.<\/li>\n<li>Acceptable level of endotoxin (10 mg\/Dl) and hemoglobin (20 mg\/Dl).<\/li>\n<li>Due to the loss of small growth factors and other essential elements below the size of 3.5 kDa we recommend using 15-20% DFBS in your media to ensure cultured cell wellbeing.<\/li>\n<\/ul>\n<h3>Dialyzed FBS vs regular FBS<\/h3>\n<table>\n<thead>\n<tr>\n<th><\/th>\n<th>Regular FBS<\/th>\n<th>Dialyzed FBS<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Small molecules below 3.5 kDa (amino acids, nucleotides, hormones, salts)<\/td>\n<td>Present<\/td>\n<td>Removed or reduced<\/td>\n<\/tr>\n<tr>\n<td>Best for<\/td>\n<td>Routine culture of most cell lines<\/td>\n<td>Defined-media, metabolic and nutrient-response studies<\/td>\n<\/tr>\n<tr>\n<td>Amount in media<\/td>\n<td>Usually 10%<\/td>\n<td>15-20% recommended, to make up for small growth factors lost in dialysis<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Choose dialyzed FBS when the small molecules in ordinary serum would interfere with what you are measuring. For routine culture, our <a href=\"\/product\/premium-fetal-bovine-serum-p-fbs\/\">Fetal Bovine Serum (P-FBS)<\/a> needs no extra processing.<\/p>\n<h3><span style=\"color: #000000;\">Analysis<\/span><\/h3>\n<p>Routine pathological analysis on PurMa bovine and horse serums: <strong><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Routin-Patalogicsl-Analysis-on-PurMa-Bovine-and-Horse-Serums.pdf\"><span style=\"color: #0000ff;\">Routine Pathological Analysis on PurMa Bovine and Horse Serums<\/span><\/a><\/strong><\/p>\n<h3>Further Instructions<\/h3>\n<p>On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.<\/p>\n<h3>References<\/h3>\n<ol>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/20362047\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Optimization of chemically defined cell culture media&#8211;replacing fetal bovine serum in mammalian in vitro methods.<\/span> <\/a>van der Valk et al. Toxicol In Vitro. 2010 Jun;24(4):1053-63. doi: 10.1016\/j.tiv.2010.03.016. Epub 2010 Mar 31. PMID: 2036204.<\/li>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6742575\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Removal of serum lipids and lipid-derived metabolites to investigate breast cancer cell biology<\/span><\/a>.Brovkovych et al. Proteomics; available in PMC 2019 Sep 12.Published in final edited formas: Proteomics. 2019 Sep; 19(18): e1800370. Published online 2019 May 7. doi: 10.1002\/pmic.201800370. PMCID: PMC6742575.<\/li>\n<\/ol>\n<table width=\"708\">\n<tbody>\n<tr>\n<td width=\"366\"><strong>Methodology<\/strong><\/td>\n<td width=\"120\"><strong>Unit measured<\/strong><\/td>\n<td width=\"108\"><strong>Accepted level<\/strong><\/td>\n<td width=\"114\"><strong>Result<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Sterility (Current USP and EP 2.6.1 for Bacteria &amp; Fungi)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">pH<\/td>\n<td width=\"120\">N\/A<\/td>\n<td width=\"108\">measured<\/td>\n<td width=\"114\">6.58<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Osmolality (USP 785)\u00a0\u00a0\u00a0\u00a0 mOsm\/KgH2<\/td>\n<td width=\"120\">mOsm\/KgH20<\/td>\n<td width=\"108\">280-340<\/td>\n<td width=\"114\">288<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Hemoglobin (Fleming &amp; Woolf)<\/td>\n<td width=\"120\">mg\/dL<\/td>\n<td width=\"108\">&lt;25<\/td>\n<td width=\"114\">12.9<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Endotoxin (USP 85)<\/td>\n<td width=\"120\">EU\/mL<\/td>\n<td width=\"108\">&lt;20<\/td>\n<td width=\"114\">&lt;0.100<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Mycoplasma (Barile &amp; Kern; Large Volume, Direct Culture)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">IgG<\/td>\n<td width=\"120\">Elliza<\/td>\n<td width=\"108\">Measured<\/td>\n<td width=\"114\">0.19 ug\/ml<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Albumin<\/td>\n<td width=\"120\">Eliza<\/td>\n<td width=\"108\">Measured<\/td>\n<td width=\"114\">\u2264 4.6 g\/dL<\/td>\n<\/tr>\n<tr>\n<td width=\"366\"><strong>Virus Testing (9 CFR 113.53c)<\/strong><\/td>\n<td width=\"120\"><\/td>\n<td width=\"108\"><\/td>\n<td width=\"114\"><\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Adenovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Parvovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Respiratory Syncytial Virus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Viral Diarrhea Virus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Rabies<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Reovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Cytopathogenic Agents (IBR)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Hemadsorbing Agents (PI3)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bluetongue<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-fetal-bovine-serum-used-for\/\">What Is Fetal Bovine Serum Used For?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fetal bovine serum dialyzed to remove small molecules, for experiments that need a more defined culture environment.<\/p>\n<ul>\n<li>Reduces low-molecular-weight components: antibiotics, nucleotides, amino acids, and hormones<\/li>\n<li>Improves consistency for defined-media and metabolic studies<\/li>\n<\/ul>\n","protected":false},"featured_media":127218,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[31,589],"product_tag":[586,647],"class_list":["post-58414","product","type-product","status-publish","has-post-thumbnail","product_cat-fetal-bovine-serum","product_cat-bovine-serums","product_tag-dialyzed-serum","product_tag-fetal-bovine-serum","entry","has-media","first","instock","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/58414","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=58414"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127218"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=58414"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=58414"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=58414"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=58414"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}