{"id":58423,"date":"2023-07-09T07:34:10","date_gmt":"2023-07-09T07:34:10","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=58423"},"modified":"2026-08-13T13:47:17","modified_gmt":"2026-08-13T19:47:17","slug":"charcoal-stripped-fetal-bovine-serum","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/charcoal-stripped-fetal-bovine-serum\/","title":{"rendered":"Charcoal-Stripped Fetal Bovine Serum (CS-FBS)"},"content":{"rendered":"<h2>Background<\/h2>\n<p>Charcoal stripped FBS (CS-FBS) is fetal bovine serum treated with activated carbon to remove hormones, steroids, and other lipophilic substances.<\/p>\n<ul>\n<li>This process removes all the non-polar material such as lipophilic (lipid-related) materials (viruses, certain growth factors, hormones, and cytokines).<\/li>\n<li>Acts as an effective blocker agent in immunoassay systems as well as immunoprecipitating analysis (by coating the Sepharose-activated beads in order to prevent nonspecific binding).<\/li>\n<li>Moreover, it performs the function regardless of the molecular weight of the target.<\/li>\n<\/ul>\n<p>PurMa Biologics applies Norit A charcoal coated with Dextran T-70 to manufacture a high-quality Charcoal-Stripped Fetal Bovine Serum.<\/p>\n<p>Not sure which serum fits your cells? See our complete guide to <a href=\"\/cell-culture-serum\/\">cell culture serum.<\/a><\/p>\n<h3>PurMa\u2122 Charcoal-Stripped FBS (<strong>CS-FBS<\/strong>)<\/h3>\n<ul>\n<li>PurMa<sup>TM<\/sup> Premium FBS is of USA origin.<\/li>\n<li>Goes through heat inactivation and gamma irradiation.<\/li>\n<li>Following the stripping, the FBS goes through heat inactivation process.<\/li>\n<li>PurMa Biologics performs vigorous analysis following the stripping and will provide a certificate of analysis.<\/li>\n<li>The procedure depletes any residue of \u00a0mycoplasma, steroids and hormones, and other pathogens.<\/li>\n<li>Acceptable level of endotoxin (10 mg\/Dl) and hemoglobin (20 mg\/Dl).<\/li>\n<li>Due to the loss of a vast variety of hormones and steroids (such as growth factors), we recommend using 15-20% CS-FBS in your media to ensure cultured cells&#8217; wellbeing.<\/li>\n<\/ul>\n<p>Routine pathological analysis on PurMa bovine and horse serums: <strong><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Routin-Patalogicsl-Analysis-on-PurMa-Bovine-and-Horse-Serums.pdf\"><span style=\"color: #0000ff;\">Routine Pathological Analysis on PurMa Bovine and Horse Serums<\/span><\/a><\/strong><\/p>\n<h3>Further Instructions<\/h3>\n<p>On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.<\/p>\n<h3>References<\/h3>\n<ol>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/1000504\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture<\/span>. <\/a>Lippman et al. Cancer Res. 1976 Dec;36(12):4595-601. PMID:<\/li>\n<li>Leake et al. <em>Steroid Hormones: A Practical Approach. <\/em>Oxford, UK: IRL Press; 1987.<\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/7053842\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Effects of estrogens and antiestrogens on estrogen receptor dynamics and the induction of progesterone receptor in MCF-7 human breast cancer cells<\/span>. <\/a>Eckert et al. Cancer Res. 1982 Jan;42(1):139-44.<\/li>\n<\/ol>\n<table width=\"708\">\n<tbody>\n<tr>\n<td width=\"366\"><strong>Methodology<\/strong><\/td>\n<td width=\"120\"><strong>Unit measured<\/strong><\/td>\n<td width=\"108\"><strong>Accepted level<\/strong><\/td>\n<td width=\"114\"><strong>Result<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Sterility (Current USP and EP 2.6.1 for Bacteria &amp; Fungi)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">pH<\/td>\n<td width=\"120\">N\/A<\/td>\n<td width=\"108\">measured<\/td>\n<td width=\"114\">6.58<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Osmolality (USP 785)\u00a0\u00a0\u00a0\u00a0 mOsm\/KgH2<\/td>\n<td width=\"120\">mOsm\/KgH20<\/td>\n<td width=\"108\">280-340<\/td>\n<td width=\"114\">288<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Hemoglobin (Fleming &amp; Woolf)<\/td>\n<td width=\"120\">mg\/dL<\/td>\n<td width=\"108\">&lt;25<\/td>\n<td width=\"114\">12.9<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Endotoxin (USP 85)<\/td>\n<td width=\"120\">EU\/mL<\/td>\n<td width=\"108\">&lt;20<\/td>\n<td width=\"114\">&lt;0.100<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Mycoplasma (Barile &amp; Kern; Large Volume, Direct Culture)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">IgG<\/td>\n<td width=\"120\">Elliza<\/td>\n<td width=\"108\">Measured<\/td>\n<td width=\"114\">0.19 ug\/ml<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Albumin<\/td>\n<td width=\"120\">Eliza<\/td>\n<td width=\"108\">Measured<\/td>\n<td width=\"114\">\u2264 4.6 g\/dL<\/td>\n<\/tr>\n<tr>\n<td width=\"366\"><strong>Virus Testing (9 CFR 113.53c)<\/strong><\/td>\n<td width=\"120\"><\/td>\n<td width=\"108\"><\/td>\n<td width=\"114\"><\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Adenovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Parvovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Respiratory Syncytial Virus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bovine Viral Diarrhea Virus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Rabies<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Reovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Cytopathogenic Agents (IBR)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Hemadsorbing Agents (PI3)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bluetongue<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-fetal-bovine-serum-used-for\/\">What Is Fetal Bovine Serum Used For?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fetal bovine serum treated with activated charcoal to remove hormones and steroids.<\/p>\n<ul>\n<li>Activated-carbon stripping removes steroids and hormones<\/li>\n<li>For studies where background hormones or steroids must be minimized<\/li>\n<\/ul>\n","protected":false},"featured_media":127221,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[31,589],"product_tag":[643,647],"class_list":["post-58423","product","type-product","status-publish","has-post-thumbnail","product_cat-fetal-bovine-serum","product_cat-bovine-serums","product_tag-charcoal-stripped","product_tag-fetal-bovine-serum","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/58423","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=58423"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127221"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=58423"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=58423"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=58423"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=58423"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}