{"id":58448,"date":"2023-07-10T05:08:21","date_gmt":"2023-07-10T05:08:21","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=58448"},"modified":"2026-10-06T23:11:43","modified_gmt":"2026-10-07T05:11:43","slug":"dialyzed-fetal-horse-serum-dfhs","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/dialyzed-fetal-horse-serum-dfhs\/","title":{"rendered":"Dialyzed Fetal Horse Serum (DFHS)"},"content":{"rendered":"<h2>Background<\/h2>\n<p>Animal serum contains many undefined components. PurMa\u2122 Dialyzed Fetal Horse Serum (DFHS) provides more consistency among the cell culture experiments. This is especially true in studies requiring a more defined environment of small molecules. The dialysis process eliminates\/ reduces the concentration of low molecular weight components. That includes antibiotics, nucleotides, amino acids, hormones, salts, and various small proteins in serum.<\/p>\n<p><strong><span style=\"color: #ff0000;\">PurMa<sup>TM<\/sup> Dialyzed FHS is Free of Tetracycline and other Antibiotics.<\/span> <\/strong><\/p>\n<h3><strong>PurMa\u2122<\/strong> <strong>Dialyzed FHS (DFHS)<\/strong><\/h3>\n<ul>\n<li>DFHS has been dialyzed using the 3500 Dalton (3.5 kDa) dialysis tube. The dialysis is carried out against 10X 10-liter PBS (pH 7.4). At the end, the dialyzed FHS is filtered three times (0.1 \u00b5M filters).<\/li>\n<li>PurMa\u2122 Dialyzed Fetal Horse Serum (DFHS) is of USA origin.<\/li>\n<li>The parent DFHS has been gamma irradiated.<\/li>\n<li>Following the dialysis, the FHS goes through heat inactivation process.<\/li>\n<li>Following the dialysis, the FHS goes through vigorous analysis and will get a certificate of analysis.<\/li>\n<li>Moreover, PurMa\u2122 Dialyzed Fetal Horse Serum (DFHS) is free of Mycoplasma, steroids and hormones and other pathogens.<\/li>\n<li>PurMa\u2122 DFHS is free of endotoxin and also contains an acceptable level of hemoglobin (20 mg\/Dl).<\/li>\n<li><strong>Due to the loss of small growth factors and other essential elements below the size of 3.5 kDa we recommend using 15-20% DFHS in your media to ensure cultured cell wellbeing. <\/strong>\n<ul>\n<li><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3><strong>Further Instructions <\/strong><\/h3>\n<p>On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.<\/p>\n<p>Routine pathological analysis on PurMa bovine and horse serums: <strong><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Routin-Patalogicsl-Analysis-on-PurMa-Bovine-and-Horse-Serums.pdf\"><span style=\"color: #0000ff;\">Routine Pathological Analysis on PurMa Bovine and Horse Serums<\/span><\/a><\/strong><\/p>\n<h3>References<\/h3>\n<ol>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/511211\/\" rel=\"nofollow noopener\" target=\"_blank\">Effect of horse serum on neural cell differentiation in tissue culture. <\/a><\/span>Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.<\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31621377\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Higher percentage of horse serum in culture media blocks attachment of PC12 cells.<\/span> <\/a>Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<table width=\"708\">\n<tbody>\n<tr>\n<td width=\"366\"><strong>Methodology<\/strong><\/td>\n<td width=\"120\"><strong>Unit measured<\/strong><\/td>\n<td width=\"108\"><strong>Accepted level<\/strong><\/td>\n<td width=\"114\"><strong>Result<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Sterility (Current USP and EP 2.6.1 for Bacteria &amp; Fungi)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">pH<\/td>\n<td width=\"120\">N\/A<\/td>\n<td width=\"108\">measured<\/td>\n<td width=\"114\">6.58<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Osmolality (USP 785)\u00a0\u00a0\u00a0\u00a0 mOsm\/KgH2<\/td>\n<td width=\"120\">mOsm\/KgH20<\/td>\n<td width=\"108\">280-340<\/td>\n<td width=\"114\">288<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Hemoglobin (Fleming &amp; Woolf)<\/td>\n<td width=\"120\">mg\/dL<\/td>\n<td width=\"108\">&lt;25<\/td>\n<td width=\"114\">12.9<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Endotoxin (USP 85)<\/td>\n<td width=\"120\">EU\/mL<\/td>\n<td width=\"108\">&lt;20<\/td>\n<td width=\"114\">&lt;0.100<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Mycoplasma (Barile &amp; Kern; Large Volume, Direct Culture)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">IgG<\/td>\n<td width=\"120\">Elliza<\/td>\n<td width=\"108\">Measured<\/td>\n<td width=\"114\">0.19 ug\/ml<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Albumin<\/td>\n<td width=\"120\">Eliza<\/td>\n<td width=\"108\">Measured<\/td>\n<td width=\"114\">\u2264 4.6 g\/dL<\/td>\n<\/tr>\n<tr>\n<td width=\"366\"><strong>Virus Testing <\/strong><\/td>\n<td width=\"120\"><\/td>\n<td width=\"108\"><\/td>\n<td width=\"114\"><\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Horse Rhinopneumonitis\/Equine Herpesvirus (EHV)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Horse Equine Encephalomyelitis<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Horse Equine Infectious Anemia Virus (EIA)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Horse West Nile Virus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Rabies<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Reovirus<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Cytopathogenic Agents (IBR)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Hemadsorbing Agents (PI3)<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\">Bluetongue<\/td>\n<td width=\"120\">NA<\/td>\n<td width=\"108\">Not Detected<\/td>\n<td width=\"114\">Not Detected<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-horse-serum\/\">What Is Horse Serum?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fetal horse serum dialyzed to remove small molecules, for experiments that need a more defined environment.<\/p>\n<ul>\n<li>Reduces low-molecular-weight components for defined-media studies<\/li>\n<li>Improves consistency across cell culture experiments<\/li>\n<\/ul>\n<p class=\"purma-twin\">Need the bovine version? See <a href=\"https:\/\/purmabiologics.com\/blog\/product\/dialyzed-fetal-bovine-serum-d-fbs\/\">Dialyzed Fetal Bovine Serum (D-FBS)<\/a>.<\/p>\n","protected":false},"featured_media":127216,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[31,590],"product_tag":[585,586,554],"class_list":["post-58448","product","type-product","status-publish","has-post-thumbnail","product_cat-fetal-bovine-serum","product_cat-horse-serums","product_tag-dialyzed-fetal-horse-serum","product_tag-dialyzed-serum","product_tag-horse-serum","entry","has-media","first","instock","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/58448","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=58448"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127216"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=58448"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=58448"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=58448"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=58448"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}