{"id":58456,"date":"2023-07-10T05:15:42","date_gmt":"2023-07-10T05:15:42","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=58456"},"modified":"2026-08-31T12:50:55","modified_gmt":"2026-08-31T18:50:55","slug":"endotoxin-depleted-fetal-horse-serum","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/endotoxin-depleted-fetal-horse-serum\/","title":{"rendered":"PurMa\u2122 Endotoxin-Depleted Fetal Horse Serum (ED-FHS)"},"content":{"rendered":"<h2 data-rm-block-id=\"block-1\">Background<\/h2>\n<p data-rm-block-id=\"block-2\">PurMa Biologics manufactures seven types of horse serum, including\u00a0 PurMa\u2122 Endotoxin Depleted Fetal Horse Serum (ED-FHS)\u00a0 each specialized for certain requirements in tissue culture:<\/p>\n<ol>\n<li data-rm-block-id=\"block-3\">PurMa\u2122 Premium Fetal Horse Serum (P-FHS).<\/li>\n<li data-rm-block-id=\"block-4\">PurMa\u2122 Ultra-Premium Fetal Horse Serum (UP-FHS).<\/li>\n<li data-rm-block-id=\"block-5\">PurMa\u2122 Endotoxin-Depleted Fetal Horse Serum (ED-FHS).<\/li>\n<\/ol>\n<p data-rm-block-id=\"block-6\">Additionally, PurMa\u2122 Endotoxin-Depleted Fetal Horse Serum (ED-FHS) is processed from PurMa\u2122 Premium Fetal Horse Serum (P-FHS).\u00a0\u00a0It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.<\/p>\n<p data-rm-block-id=\"block-7\">Not sure which serum fits your cells? See our complete guide to <a href=\"\/cell-culture-serum\/\">cell culture serum.<\/a><\/p>\n<h2 data-rm-block-id=\"block-8\">Endotoxin Removal<\/h2>\n<p data-rm-block-id=\"block-9\">PurMa Biologics removes the endotoxin from FHS\u00a0 by passing the premium FHS through the proprietary\u00a0<strong> PurMa<sup>\u2122 <\/sup>Endotoxin Elimination Column (Cat# P6H1216251). <\/strong>The Sepharose 6B in our\u00a0 column is covalently conjugated to an endotoxin-specific absorbent. The mentioned procedure follows by eliminating the residual endotoxin by passing the unbound fraction three times through 0.1\u00b5m filters.<\/p>\n<h2 data-rm-block-id=\"block-10\">Product Features<\/h2>\n<ul>\n<li data-rm-block-id=\"block-11\">Firstly, PurMa\u2122 Endotoxin Depleted Fetal Horse Serum (ED-FHS) is of USA origin.<\/li>\n<li data-rm-block-id=\"block-12\">Also, it is free of endotoxin.<\/li>\n<li data-rm-block-id=\"block-13\">Moreover, PurMa\u2122 Endotoxin Depleted Fetal Horse Serum (ED-FHS) has been gamma irradiated.<\/li>\n<li data-rm-block-id=\"block-14\">Has been heat inactivated.<\/li>\n<li data-rm-block-id=\"block-15\">And lastly, has been vigorously analyzed with the certificate of analysis.<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-16\">Routine pathological analysis on PurMa bovine and horse serums:<\/p>\n<h3 data-rm-block-id=\"block-17\">Reference<\/h3>\n<ol>\n<li data-rm-block-id=\"block-18\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/511211\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Effect of horse serum on neural cell differentiation in tissue culture.<\/span> <\/a>Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.<\/li>\n<li data-rm-block-id=\"block-19\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31621377\/\" rel=\"nofollow noopener\" target=\"_blank\">Higher percentage of horse serum in culture media blocks attachment of PC12 cells. <\/a><\/span>Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.<\/li>\n<\/ol>\n<table width=\"708\">\n<tbody>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-20\">\n<h3><strong>Methodology<\/strong><\/h3>\n<\/td>\n<td width=\"120\" data-rm-block-id=\"block-21\"><strong>Unit measured<\/strong><\/td>\n<td width=\"108\" data-rm-block-id=\"block-22\"><strong>Accepted level<\/strong><\/td>\n<td width=\"114\" data-rm-block-id=\"block-23\"><strong>Result<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-24\">Sterility (Current USP and EP 2.6.1 for Bacteria &amp; Fungi)<\/td>\n<td width=\"120\" data-rm-block-id=\"block-25\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-26\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-27\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-28\">pH<\/td>\n<td width=\"120\" data-rm-block-id=\"block-29\">N\/A<\/td>\n<td width=\"108\" data-rm-block-id=\"block-30\">measured<\/td>\n<td width=\"114\" data-rm-block-id=\"block-31\">6.58<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-32\">Osmolality (USP 785)\u00a0\u00a0\u00a0\u00a0 mOsm\/KgH2<\/td>\n<td width=\"120\" data-rm-block-id=\"block-33\">mOsm\/KgH20<\/td>\n<td width=\"108\" data-rm-block-id=\"block-34\">280-340<\/td>\n<td width=\"114\" data-rm-block-id=\"block-35\">288<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-36\">Hemoglobin (Fleming &amp; Woolf)<\/td>\n<td width=\"120\" data-rm-block-id=\"block-37\">mg\/dL<\/td>\n<td width=\"108\" data-rm-block-id=\"block-38\">&lt;25<\/td>\n<td width=\"114\" data-rm-block-id=\"block-39\">12.9<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-40\">Endotoxin (USP 85)<\/td>\n<td width=\"120\" data-rm-block-id=\"block-41\">EU\/mL<\/td>\n<td width=\"108\" data-rm-block-id=\"block-42\">&lt;20<\/td>\n<td width=\"114\" data-rm-block-id=\"block-43\">&lt;0.100<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-44\">Mycoplasma (Barile &amp; Kern; Large Volume, Direct Culture)<\/td>\n<td width=\"120\" data-rm-block-id=\"block-45\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-46\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-47\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-48\">IgG<\/td>\n<td width=\"120\" data-rm-block-id=\"block-49\">Elliza<\/td>\n<td width=\"108\" data-rm-block-id=\"block-50\">Measured<\/td>\n<td width=\"114\" data-rm-block-id=\"block-51\">0.19 ug\/ml<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-52\">Albumin<\/td>\n<td width=\"120\" data-rm-block-id=\"block-53\">Eliza<\/td>\n<td width=\"108\" data-rm-block-id=\"block-54\">Measured<\/td>\n<td width=\"114\" data-rm-block-id=\"block-55\">\u2264 4.6 g\/dL<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-56\"><strong>Virus Testing <\/strong><\/td>\n<td width=\"120\" data-rm-block-id=\"block-57\"><\/td>\n<td width=\"108\" data-rm-block-id=\"block-58\"><\/td>\n<td width=\"114\" data-rm-block-id=\"block-59\"><\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-60\">Horse Rhinopneumonitis\/Equine Herpesvirus (EHV)<\/td>\n<td width=\"120\" data-rm-block-id=\"block-61\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-62\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-63\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-64\">Horse Equine Encephalomyelitis<\/td>\n<td width=\"120\" data-rm-block-id=\"block-65\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-66\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-67\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-68\">Horse Equine Infectious Anemia Virus (EIA)<\/td>\n<td width=\"120\" data-rm-block-id=\"block-69\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-70\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-71\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-72\">Horse West Nile Virus<\/td>\n<td width=\"120\" data-rm-block-id=\"block-73\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-74\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-75\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-76\">Rabies<\/td>\n<td width=\"120\" data-rm-block-id=\"block-77\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-78\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-79\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-80\">Reovirus<\/td>\n<td width=\"120\" data-rm-block-id=\"block-81\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-82\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-83\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-84\">Cytopathogenic Agents (IBR)<\/td>\n<td width=\"120\" data-rm-block-id=\"block-85\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-86\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-87\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-88\">Hemadsorbing Agents (PI3)<\/td>\n<td width=\"120\" data-rm-block-id=\"block-89\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-90\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-91\">Not Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"366\" data-rm-block-id=\"block-92\">Bluetongue<\/td>\n<td width=\"120\" data-rm-block-id=\"block-93\">NA<\/td>\n<td width=\"108\" data-rm-block-id=\"block-94\">Not Detected<\/td>\n<td width=\"114\" data-rm-block-id=\"block-95\">Not Detected<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p data-rm-block-id=\"block-96\"><strong>Learn more:<\/strong> <a href=\"\/what-is-horse-serum\/\">What Is Horse Serum?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>PurMa\u2122 Endotoxin-Depleted Fetal Horse Serum (ED-FHS) is specifically designed to support robust and reliable cell growth by effectively removing endotoxins that can compromise cellular function. Endotoxin depletion creates a more stable and physiologically supportive environment for cell proliferation, leading to improved experimental consistency and performance.By using ED-FHS, researchers can:<\/p>\n<ul>\n<li>Reduce variability in cell culture conditions<\/li>\n<li>Enhance cell viability, growth, and productivity<\/li>\n<li>Improve the reliability and reproducibility of results, especially with endotoxin-sensitive cell lines and assays<\/li>\n<\/ul>\n<p>PurMa\u2122 ED-FHS represents a significant advancement for the culture of sensitive cell types, making it ideal for high-precision and endotoxin-sensitive applications.<\/p>\n","protected":false},"featured_media":127215,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[31,590],"product_tag":[584,554,582],"class_list":["post-58456","product","type-product","status-publish","has-post-thumbnail","product_cat-fetal-bovine-serum","product_cat-horse-serums","product_tag-endotoxin-depleted-horse-serum","product_tag-horse-serum","product_tag-premium-horse-serum","entry","has-media","first","instock","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/58456","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=58456"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127215"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=58456"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=58456"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=58456"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=58456"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}