Reproducibility and consistency regarding In vitro<\/em> cell culture experiments is a matter of concern. The inconsistency in In vitro<\/em> cell culture is related to batch-wise variations of biological materials, mainly the serum content. PurMa\u2122 Ultra-Premium Fetal Horse Serum (UP-FHS) has far less fluctuations compared to other serums in the market. That is due to the vigorous analysis of this serum and, more importantly, the age of the fetus. Moreover, PurMa\u2122 Ultra-Premium Fetal Horse Serum (UP-FHS) is obtained from very young donor equine serum of US origin, and with a proprietary method, the serum is isolated and purified without compromising the crucial elements for growth.<\/p>\n Not sure which serum fits your cells? See our complete guide to cell culture serum.<\/a><\/p>\n PurMa Biologics routinely tests PurMa\u2122Ultra-Premium Fetal Horse Serum (UP-FHS) for the following elements. That is, of course, besides the routine tests which are provided in the \u201cSerum Analysis Tab.\u201d<\/p>\n With relative order of importance: IgG, Total Protein, Iron, Percent Iron Saturation, Inorganic Phosphorus, Glucose, Alkaline Phosphatase, Potassium, Albumin, Blood Urea Nitrogen, Sodium, Calcium, Chloride, Total Bilirubin, Creatinine, Gamma Globulin, Lactate Dehydrogenase, TIBC, SGOT, SGPT.<\/p>\n Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums<\/span><\/a><\/strong><\/p>\n <\/p>\nPurMa Biologics has three main premium fetal horse serums<\/h2>\n
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The Major Preference of UP-FHS Over P-FHS<\/h2>\n
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Routine Quality Analysis<\/h2>\n
PurMa\u2122 <\/sup>Ultra-Premium FHS<\/u><\/h2>\n
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References<\/h2>\n
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\n Methodology<\/strong><\/td>\n Unit measured<\/strong><\/td>\n Accepted level<\/strong><\/td>\n Result<\/strong><\/td>\n<\/tr>\n \n Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi)<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n \n pH<\/td>\n N\/A<\/td>\n measured<\/td>\n 6.58<\/td>\n<\/tr>\n \n Osmolality (USP 785)\u00a0\u00a0\u00a0\u00a0 mOsm\/KgH2<\/td>\n mOsm\/KgH20<\/td>\n 280-340<\/td>\n 288<\/td>\n<\/tr>\n \n Hemoglobin (Fleming & Woolf)<\/td>\n mg\/dL<\/td>\n <25<\/td>\n 12.9<\/td>\n<\/tr>\n \n Endotoxin (USP 85)<\/td>\n EU\/mL<\/td>\n <20<\/td>\n <0.100<\/td>\n<\/tr>\n \n Mycoplasma (Barile & Kern; Large Volume, Direct Culture)<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n \n IgG<\/td>\n Elliza<\/td>\n Measured<\/td>\n 0.19 ug\/ml<\/td>\n<\/tr>\n \n Albumin<\/td>\n Eliza<\/td>\n Measured<\/td>\n \u2264 4.6 g\/dL<\/td>\n<\/tr>\n \n Virus Testing <\/strong><\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n \n Horse Rhinopneumonitis\/Equine Herpesvirus (EHV)<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n \n Horse Equine Encephalomyelitis<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n \n Horse Equine Infectious Anemia Virus (EIA)<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n \n Horse West Nile Virus<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n \n Rabies<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n \n Reovirus<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n \n Cytopathogenic Agents (IBR)<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n \n Hemadsorbing Agents (PI3)<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n \n Bluetongue<\/td>\n NA<\/td>\n Not Detected<\/td>\n Not Detected<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n