{"id":58877,"date":"2023-07-13T18:56:31","date_gmt":"2023-07-13T18:56:31","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&p=58877"},"modified":"2026-08-17T14:57:57","modified_gmt":"2026-08-17T20:57:57","slug":"mammalian-cryoprotective-media-with-dmso","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/mammalian-cryoprotective-media-with-dmso\/","title":{"rendered":"Mammalian Cryoprotective Media (MCM-D) with DMSO"},"content":{"rendered":"

Background<\/h2>\n

Mammalian Cryoprotective Media (MCM-D) with DMSO provides the best cryoprotection with proven 69% recovery rate (see below). Exposing cells to temperatures below 0\u00b0C without the aid of cryoprotectants typically causes cell death. During cryopreservation cooling aqueous solutions below the equilibrium freezing point inevitably result in the formation of ice crystals in the extracellular media and a decrease in the concentration of water which has two key consequences:<\/p>\n

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  1. Osmotic gradient forms across the cell membrane, which causes \u201ccell dehydration\u201d, and<\/li>\n
  2. Ice formation and abnormal concentration of solutes in the intracellular matrix, osmotic shock, and toxicity.<\/li>\n<\/ol>\n

    More Importantly, we always manufacture\u00a0 our cryoprotective media using our most sophisticated water , for more details, Click here:<\/p>\n

    PurMa\u2122 Ultra-Pure Cell Culture Suited Water<\/span><\/a><\/strong><\/p>\n

    PurMa Biologics is the leading company in manufacturing cryoprotective media. We follow two essential goals:<\/p>\n

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    1. Allowing cells to be stored at deep cryogenic temperatures with minimal cell damage and recovery with high levels of appropriate functionality and viability, and<\/li>\n
    2. Allow minimal toxicity. \u00a0Undoubtedly, this is a challenge we have overcome in the past 3 decades.<\/li>\n<\/ol>\n

      Accordingly, we manufacture several DMSO -dependent and DMSO Free cryoprotective media. PurMaTM<\/sup> (MCM) contains DMSO.<\/p>\n

      PurMaTM<\/sup> Mammalian Cryoprotective Media (MCM) has an excellent performance for short-term cold storage as well as long-term cryopreservation. Please see the below figure<\/p>\n

      \"Mammalian<\/p>\n

      DMSO benefits cryoprotection and cryoprotective agent in several ways<\/h2>\n
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      1. Provides strong molecular stability.<\/li>\n
      2. Besides, facilitates a high non-conductor constant.<\/li>\n
      3. It has the basic property of salt solubility, particularly for anions. Additionally, it has a propensity to act as the acceptor atom in hydrogen bonding.<\/li>\n
      4. Especially, \u00a0DMSO makes a \u00a0hydrogen bond with water which is 30% stronger than between two water molecules.<\/li>\n
      5. The proprietary formulation of PurMaTM<\/sup> Mammalian Cryoprotective Media (MCM) allows the MCM to get inside the cells instantly and lower cell death because of penetration\/freezing.<\/li>\n<\/ol>\n

        Special Features<\/h2>\n