- \n
- Osmotic gradient forms across the cell membrane, which causes \u201ccell dehydration\u201d, and<\/li>\n
- Ice formation and abnormal concentration of solutes in the intracellular matrix, osmotic shock, and toxicity.<\/li>\n<\/ol>\n
PurMa Biologics, with decades of experience in cutting-edge science, has manufactured PurMa\u2122 R Cells Cryoprotective Media (RCCM) With DMSO\u00a0 which gives a high survival and recovery rate for reproductive cells.<\/p>\n
There are several criteria that make DMSO a powerful cryoprotective agent<\/h2>\n
- \n
- PurMa\u2122 R -Cell Cryoprotective Media (RCCM-D) provides strong molecular stability.<\/li>\n
- Besides, facilitates a high non-conductor constant.<\/li>\n
- It has the basic property of salt solubility, particularly for anions. Additionally, it has a propensity to act as the acceptor atom in hydrogen bonding.<\/li>\n
- Especially, DMSO makes a \u00a0hydrogen bond with water which is 30% stronger than between two water molecules.<\/li>\n
- The proprietary formulation of PurMa\u2122 RCCM-D allows the cryoprotective agent to get inside the cells instantly and lower cell death because of penetration\/freezing.<\/li>\n<\/ol>\n
![\"R](\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/RCCM-D-cryoprotective-media-with-DMSO.jpg.webp\")
<\/p>\nPurMa\u2122 R -Cell Cryoprotective Media (RCCM-D) penetrates inside the cells instantly and lower cell death.<\/p>\n
Special Features for R Cells Cryoprotective Media (RCCM) With DMSO<\/h2>\n
- \n
- Ready-to-Use medium.<\/li>\n
- Each Lot of our PurMaTM<\/sup> RCCM-D with DMSO goes through pathogens and sterility evaluations.<\/li>\n
- We evaluate each Lot for survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), as well as for the rate of recovery by Alamar Blue test.<\/li>\n
- R Cells Cryoprotective Media (RCCM) With DMSO results in the lowest cell loss during freezing and thawing as well as the highest cell recovery. Moreover, it provides over 66% survival and recovery rate after long term cryopreservation.<\/li>\n
- Sterile (Passed through 0.2\u00b5m filters).<\/li>\n
- Moreover, PurMaTM<\/sup> RCCM-D provides an exceptional cryopreservation for primary and commercial cell lines.<\/li>\n
- Suitable for clinical applications.<\/li>\n<\/ul>\n
Guideline R Cells Cryoprotective Media (RCCM) With DMSO<\/h2>\n
For PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/span><\/a><\/strong><\/p>\n
References<\/strong><\/h2>\n
- \n
- Fertilization of human oocytes following cryopreservation; normal karyotypes and absence of stray chromosomes. <\/a>Gook et al. Hum Reprod. 1994 Apr;9(4):684-91. doi:10.1093\/ oxfordjournals .humrep .a138572.<\/li>\n
- Cryopreservation of single human spermatozoa. <\/a>Cohen et al. Hum Reprod. 1997 May;12(5):994-1001. doi: 10.1093\/humrep\/12.5.994.<\/li>\n<\/ol>\n
\n\n\n
\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n <\/p>\n
- Cryopreservation of single human spermatozoa. <\/a>Cohen et al. Hum Reprod. 1997 May;12(5):994-1001. doi: 10.1093\/humrep\/12.5.994.<\/li>\n<\/ol>\n