With decades of expertise in stem cell biology, biochemistry, and cell culture, PurMa Biologics has developed a next-generation cryoprotective media specialized for delicate stem cell culture technology. PurMa\u2122 SCCM-D contains DMSO and gives the best preservation for stem cells, particularly proven successfully on hematopoietic, mesenchymal stromal, and induced pluripotent stem cells.<\/p>\n
![\"\"](\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Stem-Cell-cryoprotective-media-with-DMSO_SCCM.jpg.webp\")
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Special Features<\/h2>\n\n- Ready-to-Use.<\/li>\n
- Moreover, PurMa\u2122 SCCM-D provides an exceptional cryopreservation for primary and commercial cell lines.<\/li>\n
- PurMa\u2122 SCCM-D With DMSO provides much higher cell viability after short\/long-term cryoprotection, (66% recovery rate)<\/li>\n<\/ul>\n
\n- PurMaTM<\/sup> SCCM-D prevents the formation of crystals in extracellular space. This, consequently, prevents cellular dehydration and results in less exposure to toxins from the non-recovering cells.<\/li>\n
- Preserves morphological appearance as a standard factor of cell recovery.<\/li>\n
- We test PurMa\u2122 SCCM-D for pH, osmolality, sterility, and endotoxin.<\/li>\n
- Each Lot of PurMaTM<\/sup> SCCM-D goes through several important tests. Those are\u00a0 a survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and the rate of recovery by Alamar Blue test.<\/li>\n
- PurMaTM<\/sup> SCCM-D has been tested successfully for several commonly used stem cells. The most commonly used include hematopoietic, mesenchymal stromal\/stem, and induced pluripotent stem cells.<\/li>\n
- Suitable for clinical applications.<\/li>\n<\/ul>\n
Guideline<\/h2>\n
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/span><\/a><\/strong><\/p>\nReferences<\/h2>\n\n- Long-term preservation of permanent human hematopoietic cell lines<\/span>. <\/a>Mizrahi et al. J Med. 1971; 2(6) :380-6.<\/li>\n
- Survival of human bone marrow progenitor cells after freezing: improved detection in the colony-formation assay<\/span>. <\/a>Schlunk et al. 1981 Apr;18(2):111-8. doi: 10.1016\/0011-2240(81)90082-1<\/li>\n<\/ol>\n\n
\n\n\nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n\nAppearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \npH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \nOsmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \nEndotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \nMycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \nSuitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \nAdditive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \nIndicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \nMycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \nSterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \nForm<\/td>\n Liquid<\/td>\n<\/tr>\n \nShipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n <\/p>\n
- \n
- PurMaTM<\/sup> SCCM-D prevents the formation of crystals in extracellular space. This, consequently, prevents cellular dehydration and results in less exposure to toxins from the non-recovering cells.<\/li>\n
- Preserves morphological appearance as a standard factor of cell recovery.<\/li>\n
- We test PurMa\u2122 SCCM-D for pH, osmolality, sterility, and endotoxin.<\/li>\n
- Each Lot of PurMaTM<\/sup> SCCM-D goes through several important tests. Those are\u00a0 a survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and the rate of recovery by Alamar Blue test.<\/li>\n
- PurMaTM<\/sup> SCCM-D has been tested successfully for several commonly used stem cells. The most commonly used include hematopoietic, mesenchymal stromal\/stem, and induced pluripotent stem cells.<\/li>\n
- Suitable for clinical applications.<\/li>\n<\/ul>\n
Guideline<\/h2>\n
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/span><\/a><\/strong><\/p>\n
References<\/h2>\n
- \n
- Long-term preservation of permanent human hematopoietic cell lines<\/span>. <\/a>Mizrahi et al. J Med. 1971; 2(6) :380-6.<\/li>\n
- Survival of human bone marrow progenitor cells after freezing: improved detection in the colony-formation assay<\/span>. <\/a>Schlunk et al. 1981 Apr;18(2):111-8. doi: 10.1016\/0011-2240(81)90082-1<\/li>\n<\/ol>\n
\n\n\n
\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n <\/p>\n
- Survival of human bone marrow progenitor cells after freezing: improved detection in the colony-formation assay<\/span>. <\/a>Schlunk et al. 1981 Apr;18(2):111-8. doi: 10.1016\/0011-2240(81)90082-1<\/li>\n<\/ol>\n