{"id":58894,"date":"2023-07-13T19:31:48","date_gmt":"2023-07-13T19:31:48","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=58894"},"modified":"2026-07-31T14:16:56","modified_gmt":"2026-07-31T20:16:56","slug":"whole-tissue-cryoprotective-media-with-dmso","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/whole-tissue-cryoprotective-media-with-dmso\/","title":{"rendered":"Whole Tissue Cryoprotective Media (WTCM-D) with DMSO"},"content":{"rendered":"<h2>Background<\/h2>\n<p>Cryopreservation of tissues especially when a big portion is intended to be used is challenging. Additionally, the biggest obstacle is forming ice crystals inside the cells. \u00a0This inevitably causes volume expansion with the destruction of cell membranes and holes in loose connective tissue. Whole Tissue Cryoprotective Media (WTCM-D) with DMSO preserves tissues during rapid freezing. This remarkably promotes \u00a0formation of amorphous ice ( rather than crystal ice).<\/p>\n<h2>PurMa\u2122 WTCM-D \u00a0has particularly overcome several challenges<\/h2>\n<ol>\n<li>Liquid nitrogen has a low specific heat constant. \u00a0When it contacts warm tissues or OCT it boils and forms a vapor barrier and acts as an insulator. As a result, the inner core of the tissue sample freezes slowly and unevenly. This is especially more challenging when the tissue sample is large. The cryoprotective agents in PurMa\u2122 WTCM-D prevent this from happening by penetrating rapidly inside the tissue.<\/li>\n<li>PurMa\u2122 WTCM-D acts regardless of the size of the tissue. PurMa\u2122 WTCM has high thermal conductivity. This, therefore, allows for rapid and efficient cryoprotection using liquid nitrogen.<\/li>\n<li>PurMa<sup>TM<\/sup> WTCM contains ingredients which make it suitable for the preservation of rigid tissues.<\/li>\n<\/ol>\n<h2>Special Features<\/h2>\n<ul>\n<li>Ready-to-Use.<\/li>\n<li>PurMa<sup>TM<\/sup> WTCM provides much higher cell viability after short\/long-term cryoprotection,<\/li>\n<li>PurMa<sup>TM<\/sup> WTCM Prevents the formation of crystals in extracellular space. This, consequently, prevents cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.<\/li>\n<li>Preserves morphological appearance as a standard factor of cell recovery.<\/li>\n<li>Tested for pH, osmolality, sterility, and endotoxin.<\/li>\n<li>Each Lot of PurMa<sup>TM<\/sup> WTCM is tested for pathogens and sterility. We evaluate each Lot as well for several criteria, including survival (by Trypan Blue method), viability &amp; performance (by MTT Test), and the recovery rate by Alamar Blue test.<\/li>\n<li>PurMa<sup>TM<\/sup> WTCM has been tested successfully for several commonly used tissue including rodent heart, brain, and kidney.<\/li>\n<\/ul>\n<h2>Guideline<\/h2>\n<p>for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab:<span style=\"color: #0000ff;\"> <strong><a style=\"color: #0000ff;\" href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/PurMaBiologics-Guideline-for-Cryoprotective-Cell-Type-Selection.pdf\" target=\"_blank\" rel=\"noopener\">PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/a><\/strong><\/span><\/p>\n<h2>References<\/h2>\n<ol>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/5499041\/\" rel=\"nofollow noopener\" target=\"_blank\">Diffusion and distribution of dimethyl sulphoxide in the isolated guinea-pig taenia coli. <\/a><\/span>Elford BC. J Physiol. 1970 Jul;209(1):187-208. doi: 10.1113\/jphysiol.1970.sp009162.<\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25975010\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Cryopreservation of whole ovine ovaries with pedicles as a model for human: parameters of perfusion with simultaneous saturations by cryoprotectants.<\/span> <\/a>Isachenko et al. Clin Lab. 2015;61(3-4):415-20. doi: 10.7754\/clin.lab.2014.140919. PMID:<\/li>\n<\/ol>\n<div class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\">Parameter<\/th>\n<th align=\"left\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\">Appearance<\/td>\n<td align=\"left\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">pH<\/td>\n<td align=\"left\">\u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Osmolality<\/td>\n<td align=\"left\">\u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Endotoxin<\/td>\n<td align=\"left\">\u00a0NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Mycoplasma<\/td>\n<td align=\"left\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Suitability<\/td>\n<td align=\"left\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Additive<\/td>\n<td align=\"left\">\u00a0Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Indicator<\/td>\n<td align=\"left\">\u00a0Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Mycoplasma Detection<\/td>\n<td align=\"left\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Sterility Tested<\/td>\n<td align=\"left\">\u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Form<\/td>\n<td align=\"left\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Shipping Condition<\/td>\n<td align=\"left\">\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>&nbsp;<\/p>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-cryoprotective-media\/\">What Is Cryoprotective Media?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cryopreservation of tissues especially when a big portion is intended to be used is challenging. Additionally, the biggest obstacle is forming ice crystals inside the cells. \u00a0This inevitably, cause volume expansion with the destruction of cell membranes and holes in loose connective tissue. Whole Tissue Cryoprotective Media (WTCM-D) with DMSO, preserve tissues during rapid freezing. This remarkably promotes \u00a0formation of amorphous ice ( rather than crystal ice).<\/p>\n","protected":false},"featured_media":127226,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[58,587],"product_tag":[524,538,537],"class_list":["post-58894","product","type-product","status-publish","has-post-thumbnail","product_cat-cryoprotective-media","product_cat-cryoprotective-media-with-dmso","product_tag-cryoprotective-media","product_tag-whole-tissue","product_tag-with-dmso","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/58894","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=58894"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127226"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=58894"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=58894"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=58894"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=58894"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}