DMSO benefits cryoprotection and cryoprotective agents in several ways<\/h2>\n\n- Provides strong molecular stability.<\/li>\n
- Besides, facilitates a high non-conductor constant.<\/li>\n
- It has the basic property of salt solubility, particularly for anions. Additionally, it has a propensity to act as the acceptor atom in hydrogen bonding.<\/li>\n
- Especially, \u00a0DMSO makes a \u00a0hydrogen bond with water which is 30% stronger than between two water molecules.<\/li>\n<\/ol>\n
The proprietary formulation of PurMaTM<\/sup> HCM allows this media to get inside the cells instantly and lower cell death.<\/p>\n![\"\"](\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Hybridoma-cryoprotective-media-with-DMSO.jpg.webp\")
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Special Features<\/h2>\n\n- Ready-to-Use medium.<\/li>\n
- PurMaTM<\/sup> Hybridoma Cryoprotective Media (HCM) with DMSO provides much higher cell viability after short\/long-term cryoprotection.<\/li>\n
- PurMaTM<\/sup> HCM Prevents the formation of crystals in extracellular space. This, consequently, prevents cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.<\/li>\n
- Preserves morphological appearance as a standard factor of cell recovery.<\/li>\n
- Tested for pH, osmolality, sterility, and endotoxin.<\/li>\n
- Each Lot of PurMaTM<\/sup> HCM goes through rigorous testing for pathogens and sterility. We evaluate each Lot as well for several criteria, including survival (by Trypan Blue method), viability & performance (by MTT Test), and the recovery rate by Alamar Blue test.<\/li>\n
- PurMaTM<\/sup> HCM has been tested successfully for several antibody making cell lines including fused NS1 and CHO cells.<\/li>\n
- Suitable for clinical applications.<\/li>\n<\/ul>\n
Guideline<\/h2>\n
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/a><\/strong><\/span><\/p>\nReferences<\/h2>\n\n- The chemical and physical properties of DMSO. <\/a><\/span>MacGregor WS. Ann N Y Acad Sci. 1967 Mar 15;141(1):3-12. doi: 10.1111\/j.1749-6632.1967.tb34860.x.<\/li>\n
- Improvement of monoclonal antibody production in hybridoma cells by dimethyl sulfoxide. <\/a><\/span>Ling et al. Biotechnol Prog. 2003 Jan-Feb;19(1):158-62. doi: 10.1021\/bp020068d.<\/li>\n<\/ol>\n
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\n\n\n\nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n\nAppearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \npH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \nOsmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \nEndotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \nMycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \nSuitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \nAdditive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \nIndicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \nMycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \nSterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \nForm<\/td>\n Liquid<\/td>\n<\/tr>\n \nShipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n <\/p>\n
The proprietary formulation of PurMaTM<\/sup> HCM allows this media to get inside the cells instantly and lower cell death.<\/p>\n for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/a><\/strong><\/span><\/p>\n <\/p>\n <\/p>\n<\/p>\nSpecial Features<\/h2>\n
\n
Guideline<\/h2>\n
References<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n