Disadvantages of the Serum in Cryoprotective Media<\/h2>\n
Serum and its components are well-known for their beneficial effect on cells during cryopreservation. Nevertheless, there are known disadvantages of serum in cryoprotective media:<\/p>\n
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- The inability to fully sterilize them.<\/li>\n
- They contain an unknown composition.<\/li>\n
- The variation from batch to batch. This results in inconsistency in the rate of survival, recovery, and viability in cryopreservation.<\/li>\n<\/ol>\n
Therefore, a chemically defined Cryoprotective media resolves the several restrictions including the above-mentioned issues.<\/p>\n
Although DMSO, with its penetrating ability, is a strong cryoprotective agent, there are, however, some limitations and disadvantages. For more details about the beneficial role of DMSO, \u00a0please see our other cryoprotective media with DMSO, such as:<\/p>\n
limitations and disadvantages of DMSO<\/h2>\n
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- Basically, it has a broad toxicity.<\/li>\n
- Additionally, it modifies the expression of cells such an NK and T cells<\/li>\n
- It alters the mRNA level of the de novo<\/em> DNA methyltransferase DNMT3A. This, therefore, is accompanied by hyper- or hypo-methylation of many genetic loci.<\/li>\n
- Moreover, it causes difficulties in scaling up DMSO-based cryoprotectants.<\/li>\n
- It damages the mitochondria in certain cell culture models such as astrocytes.<\/li>\n
- It negatively impacts cellular membrane\/cytoskeleton structure integrity by interacting with proteins and dehydrating lipids.<\/li>\n
- Besides, it induces unwanted stem cell differentiation.<\/li>\n
- Affects cellular epigenetic profile resulting in undesirable phenotypic disturbances. That is basically because of repeated usage of DMSO even at sub-toxic levels.<\/li>\n
- Afterwards, it is necessary to remove DMSO by intensive washing. That in turn results in cell sensitivity to osmotic\/mechanical stresses occurring. This is a costly and time-consuming process during freezing and thawing, and moreover leads to a significant loss of cell number.<\/li>\n<\/ol>\n
PurMa\u2122 Serum Free Cryoprotective Media DMSO Free (SFCM) prevents cell dehydration and formation of ice crystals. So, it makes an excellent choice for short- and long-term cryopreservation. Moreover, PurMaTM<\/sup> SFCM allows this media to preserve your sensitive cells without penetrating the cells.<\/p>\n
Please follow the exact protocol as using non penetrating cryomedia requires slow freezing (1\u00b0C\/ min)<\/strong><\/p>\n
![\"Serum](\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Serum-Free-DMSO-Free-Cryoprotevtive-medium.jpg.webp\")
<\/p>\nSpecial Features<\/h2>\n
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- Ready-to-Use medium.<\/li>\n
- It is free of serum and DMSO ( or any other penetrating cryoprotective agents)<\/li>\n
- Each and every Lot of PurMaTM<\/sup> Serum Free Cryoprotective Medium (SFCM), besides the pathogens and sterility evaluations, goes through a survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and testing for the rate of recovery by Alamar Blue test.<\/li>\n
- PurMaTM<\/sup> Serum Free Cryoprotective Medium (SFCM) has been designed for the lowest cell loss during freezing and thawing & highest cell recovery and is proven to have over 62% survival and recovery rate after long term cryopreservation.<\/li>\n
- Sterile (Passed through 0.2\u00b5m filters).<\/li>\n
- PurMaTM<\/sup> Serum Free Cryoprotective Media (SFCM); DMSO Free has proven successful for primary and commercial cell lines.<\/li>\n
- Suitable for clinical applications.<\/li>\n<\/ul>\n
Guideline<\/h2>\n
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab:<\/span> PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/span><\/a><\/span><\/h2>\n
References<\/h2>\n
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- Cryopreservation of stromal vascular fraction of adipose tissue in a serum-free freezing medium.<\/span> <\/a>Thirumala et al. J Tissue Eng Regen Med. 2010 Mar;4(3):224-32. doi: 10.1002\/term.232. PMID: 19967746.<\/li>\n
- Phase transitions and mechanisms of cryoprotection of serum-\/xeno-free media based ondextran and dimethyl sulfoxide.<\/span> <\/a>Pakhomov et al. Cryobiology. 2022 Aug;107:13-22. doi: 10.1016\/j.cryobiol.2022.06.004. Epub 2022 Jun 24.<\/li>\n<\/ol>\n
<\/p>\n
\n\n\n
\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n <\/p>\n
- Phase transitions and mechanisms of cryoprotection of serum-\/xeno-free media based ondextran and dimethyl sulfoxide.<\/span> <\/a>Pakhomov et al. Cryobiology. 2022 Aug;107:13-22. doi: 10.1016\/j.cryobiol.2022.06.004. Epub 2022 Jun 24.<\/li>\n<\/ol>\n
- PurMaTM<\/sup> Serum Free Cryoprotective Medium (SFCM) has been designed for the lowest cell loss during freezing and thawing & highest cell recovery and is proven to have over 62% survival and recovery rate after long term cryopreservation.<\/li>\n