Cryopreservation of tissues, especially when they are large, could be challenging. Moreover, the biggest obstacle is forming ice crystals. Undeniably, it causes volume expansion with the destruction of cell membranes and holes in loose connective tissue. Whole Tissue Cryoprotective Media (WTCM); DMSO Free properly penetrates the tissue and rapidly lowers the temperature inside the tissue. Under the DMSO Free condition, tissues must be frozen at the rate of 1\u00b0C\/ min.<\/p>\n
Tissues should be frozen as rapidly as possible to promote the formation of amorphous ice (rather than crystal ice). With a low specific heat constant, liquid nitrogen, when it contacts warm tissues, boils and forms a vapor barrier which acts as an insulator. Consequently, the inner core of the tissue sample freezes slowly and unevenly. The PurMa\u2122 WTCM has high thermal conductivity and therefore, has overcome the mentioned problem.<\/p>\n
PurMa Biologics has developed a DMSO-free whole-tissue cryoprotective media which is the outcome of many years of cutting-edge science. The cryoprotectant in our media is a complex of heavy chain sugars. This combination adequately freezes down the tissue with minimal ice formation and extracellular hydration.<\/p>\n
Please read our protocol before using DMSO Free PurMa\u2122 WTCM Cryoprotective Media as following the protocol is crucial for proper cryopreservation.<\/strong><\/span><\/p>\n for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/span><\/a><\/p>\n <\/p>\nPurMa\u2122 Whole Tissue Cryoprotective Media (WTCM)<\/h2>\n
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Guideline<\/h2>\n
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\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n