- \n
- Basically, it has a broad toxicity.<\/li>\n
- Additionally, it modifies the expression of cells such an NK and T cells<\/li>\n
- It alters the mRNA level of the de novo<\/em> DNA methyltransferase DNMT3A. This, therefore, is accompanied by hyper- or hypo-methylation of many genetic loci.<\/li>\n
- Moreover, it causes difficulties in scaling up DMSO-based cryoprotectants.<\/li>\n
- It damages the mitochondria in certain cell culture models such as astrocytes.<\/li>\n
- It negatively impacts cellular membrane\/cytoskeleton structure integrity by interacting with proteins and dehydrating lipids.<\/li>\n
- Besides, it induces unwanted stem cell differentiation.<\/li>\n
- Affects cellular epigenetic profile resulting in undesirable phenotypic disturbances. That is basically because of repeated usage of DMSO even at sub-toxic levels.<\/li>\n
- Afterwards, it is necessary to remove DMSO by intensive washing. That in turn results in cell sensitivity to osmotic\/mechanical stresses occurring. This is a costly and time-consuming process during freezing and thawing, and moreover leads to a significant loss of cell number.<\/li>\n<\/ol>\n
PurMa\u2122 DMSO Free Stem Cell Cryoprotective Media provides high post-thaw cell viability and preserves original morphology, proliferation, and differentiation potentials of tested stem cells such as MSCs.<\/p>\n
![\"Stem](\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Stem-Cell-Cryoprotective-Media_DMSO-Free-.jpg.webp\")
<\/p>\nSpecial Features<\/h2>\n
- \n
- Ready-to-Use.<\/li>\n
- PurMaTM<\/sup> DMSO Free SCCM provides much higher cell viability after short\/long-term cryoprotection, (61% recovery rate)<\/li>\n
- PurMaTM<\/sup> DMSO Free SCCM Prevents the formation of crystals in extracellular space, consequently, preventing cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.<\/li>\n
- Preserves morphological appearance as a standard factor of cell recovery.<\/li>\n
- Tested for pH, osmolality, sterility, and endotoxin.<\/li>\n
- Each Lot of PurMaTM<\/sup> SCCM besides the pathogens and sterility evaluations goes through a survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and the rate of recovery by Alamar Blue test.<\/li>\n
- PurMaTM<\/sup> DMSO Free SCCM has been tested successfully for several commonly used stem cells including hematopoietic, mesenchymal stromal\/stem, and induced pluripotent stem cells.<\/li>\n
- Suitable for clinical applications.<\/li>\n<\/ul>\n
Guideline<\/h2>\n
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/span><\/a><\/strong><\/p>\n
References<\/h2>\n
- \n
- Evaluation of polyvinylpyrrolidone as a cryoprotectant for adipose tissue-derived adult stem cells. <\/a><\/span>Thirumala et al. Tissue Eng Part C Methods. 2010 Aug;16(4):783-92. doi:10.1089\/ten .TEC.2009.0552.<\/li>\n
- Long-term preservation of permanent human hematopoietic cell lines.<\/span> <\/a>Mizrahi et al. J Med. 1971; 2(6) :380-6.<\/li>\n<\/ol>\n
<\/p>\n
\n\n\n
\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n <\/p>\n
- Long-term preservation of permanent human hematopoietic cell lines.<\/span> <\/a>Mizrahi et al. J Med. 1971; 2(6) :380-6.<\/li>\n<\/ol>\n
- PurMaTM<\/sup> DMSO Free SCCM Prevents the formation of crystals in extracellular space, consequently, preventing cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.<\/li>\n