DMSO benefits cryoprotection and cryoprotective agent in several ways<\/h2>\n\n- Provides strong molecular stability.<\/li>\n
- Besides, facilitates a high non-conductor constant.<\/li>\n
- It contains the basic properties, salts solubility particularly for anions. Additionally contains a propensity to act as the acceptor atom in hydrogen bonding.<\/li>\n
- Especially, \u00a0DMSO makes a \u00a0hydrogen bond with water which is 30% stronger than between two water molecules.<\/li>\n
- The proprietary formulation of PurMaTM<\/sup> Mammalian Cryoprotective Media (MCM) allows the MCM to get inside the cells instantly and lower cell death because of penetration\/freezing.<\/li>\n<\/ol>\n
Although, DMSO with the penetrating ability, is a strong cryoprotective agent, there is however some limitations and disadvantages. For more details about the beneficial role of DMSO, \u00a0please see our other cryoprotective media with DMSO, such as:<\/p>\n
limitations and disadvantages of DMSO<\/h2>\n\n- Basically, it has a broad toxicity.<\/li>\n
- Additionally, it modifies the expression of cells such an NK and T cells<\/li>\n
- It alters the mRNA level of the de novo<\/em> DNA methyltransferase DNMT3A. This, therefore, is accompanied by hyper- or hypo-methylation of many genetic loci.<\/li>\n
- Moreover, it causes difficulties in scaling up DMSO-based cryoprotectants.<\/li>\n
- It damages the mitochondria in certain cell culture models such as astrocytes.<\/li>\n
- It negatively impacts cellular membrane\/cytoskeleton structure integrity by interacting with proteins and dehydrating lipids.<\/li>\n
- Besides, it induces unwanted stem cell differentiation.<\/li>\n
- Affects cellular epigenetic profile resulting in undesirable phenotypic disturbances. That is basically because of repeated usage of DMSO even at sub-toxic levels.<\/li>\n
- Afterwards, it is necessary to remove DMSO by intensive washing. That in turn results in cell sensitivity to osmotic\/mechanical stresses occurring. This is a costly and time-consuming process during freezing and thawing, and moreover leads to a significant loss of cell number.<\/li>\n<\/ol>\n
PurMaTM <\/sup>Hybridoma Cryoprotective Media (HCM); DMSO Free provides high post-thaw cell viability. Besides, it preserves morphology, proliferation as well as \u00a0the differentiation potentials of hybridoma cells.<\/p>\n![\"Hybridoma](\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Hybridoma-DMSO-Free-cry.-media.jpg.webp\")
<\/p>\n
Special Features<\/h2>\n\n- Ready-to-Use medium.<\/li>\n
- It does not contain DMSO or any other penetrating agents.<\/li>\n
- Each Lot of PurMaTM<\/sup> HCM goes through pathogens and sterility evaluations.<\/li>\n
- Additionally, we perform the following survival tests on each Lot of PurMaTM<\/sup> HCM: \u00a0Survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and finally, \u00a0for the rate of recovery by Alamar Blue test.<\/li>\n
- Moreover, PurMaTM<\/sup> HCM has been designed for the lowest cell loss during freezing and thawing.<\/li>\n
- PurMaTM<\/sup> HCM yields highest cell recovery, 64% survival and recovery rate after long term cryopreservation.<\/li>\n
- PurMaTM<\/sup> HCM gives faster recovery which results in less exposure to the toxins from the non-recovering cells.<\/li>\n
- Preserves morphological appearance as the main recovery factor for cells.<\/li>\n
- Tested for pH, osmolality, sterility, and endotoxin.<\/li>\n
- PurMaTM<\/sup> HCM has been tested successfully for several antibody making cell lines including fused NS1 and CHO cells.<\/li>\n
- Suitable for clinical applications.<\/li>\n<\/ul>\n
Guideline<\/h2>\n
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/span><\/a><\/p>\nReferences<\/h2>\n\n- Differentiation of erythroleukemic cells in vitro: irreversible induction by dimethyl sulfoxide (DMSO). <\/a><\/span>Preisler et al. 1975 Jun;85(3):537-46. doi: 10.1002\/jcp.1040850305.<\/li>\n
- Dimethyl sulfoxide-free cryopreservation solutions for hematopoietic stem cell grafts. <\/a><\/span>Kaushal et al. 2022 Mar;24(3):272-281. doi: 10.1016\/j.jcyt.2021.09.002. Epub 2021 Oct 12.<\/li>\n<\/ol>\n
<\/p>\n
\n\n\n\nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n\nAppearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \npH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \nOsmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \nEndotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \nMycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \nSuitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \nAdditive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \nIndicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \nMycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \nSterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \nForm<\/td>\n Liquid<\/td>\n<\/tr>\n \nShipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n <\/p>\n
Although, DMSO with the penetrating ability, is a strong cryoprotective agent, there is however some limitations and disadvantages. For more details about the beneficial role of DMSO, \u00a0please see our other cryoprotective media with DMSO, such as:<\/p>\n
limitations and disadvantages of DMSO<\/h2>\n\n- Basically, it has a broad toxicity.<\/li>\n
- Additionally, it modifies the expression of cells such an NK and T cells<\/li>\n
- It alters the mRNA level of the de novo<\/em> DNA methyltransferase DNMT3A. This, therefore, is accompanied by hyper- or hypo-methylation of many genetic loci.<\/li>\n
- Moreover, it causes difficulties in scaling up DMSO-based cryoprotectants.<\/li>\n
- It damages the mitochondria in certain cell culture models such as astrocytes.<\/li>\n
- It negatively impacts cellular membrane\/cytoskeleton structure integrity by interacting with proteins and dehydrating lipids.<\/li>\n
- Besides, it induces unwanted stem cell differentiation.<\/li>\n
- Affects cellular epigenetic profile resulting in undesirable phenotypic disturbances. That is basically because of repeated usage of DMSO even at sub-toxic levels.<\/li>\n
- Afterwards, it is necessary to remove DMSO by intensive washing. That in turn results in cell sensitivity to osmotic\/mechanical stresses occurring. This is a costly and time-consuming process during freezing and thawing, and moreover leads to a significant loss of cell number.<\/li>\n<\/ol>\n
PurMaTM <\/sup>Hybridoma Cryoprotective Media (HCM); DMSO Free provides high post-thaw cell viability. Besides, it preserves morphology, proliferation as well as \u00a0the differentiation potentials of hybridoma cells.<\/p>\n<\/p>\n
Special Features<\/h2>\n\n- Ready-to-Use medium.<\/li>\n
- It does not contain DMSO or any other penetrating agents.<\/li>\n
- Each Lot of PurMaTM<\/sup> HCM goes through pathogens and sterility evaluations.<\/li>\n
- Additionally, we perform the following survival tests on each Lot of PurMaTM<\/sup> HCM: \u00a0Survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and finally, \u00a0for the rate of recovery by Alamar Blue test.<\/li>\n
- Moreover, PurMaTM<\/sup> HCM has been designed for the lowest cell loss during freezing and thawing.<\/li>\n
- PurMaTM<\/sup> HCM yields highest cell recovery, 64% survival and recovery rate after long term cryopreservation.<\/li>\n
- PurMaTM<\/sup> HCM gives faster recovery which results in less exposure to the toxins from the non-recovering cells.<\/li>\n
- Preserves morphological appearance as the main recovery factor for cells.<\/li>\n
- Tested for pH, osmolality, sterility, and endotoxin.<\/li>\n
- PurMaTM<\/sup> HCM has been tested successfully for several antibody making cell lines including fused NS1 and CHO cells.<\/li>\n
- Suitable for clinical applications.<\/li>\n<\/ul>\n
Guideline<\/h2>\n
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/span><\/a><\/p>\nReferences<\/h2>\n\n- Differentiation of erythroleukemic cells in vitro: irreversible induction by dimethyl sulfoxide (DMSO). <\/a><\/span>Preisler et al. 1975 Jun;85(3):537-46. doi: 10.1002\/jcp.1040850305.<\/li>\n
- Dimethyl sulfoxide-free cryopreservation solutions for hematopoietic stem cell grafts. <\/a><\/span>Kaushal et al. 2022 Mar;24(3):272-281. doi: 10.1016\/j.jcyt.2021.09.002. Epub 2021 Oct 12.<\/li>\n<\/ol>\n
<\/p>\n
\n\n\n\nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n\nAppearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \npH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \nOsmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \nEndotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \nMycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \nSuitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \nAdditive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \nIndicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \nMycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \nSterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \nForm<\/td>\n Liquid<\/td>\n<\/tr>\n \nShipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n <\/p>\n
PurMaTM <\/sup>Hybridoma Cryoprotective Media (HCM); DMSO Free provides high post-thaw cell viability. Besides, it preserves morphology, proliferation as well as \u00a0the differentiation potentials of hybridoma cells.<\/p>\n for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa\u2122 Guideline for Cryoprotecting various cell lines<\/span><\/a><\/p>\n <\/p>\n <\/p>\n<\/p>\nSpecial Features<\/h2>\n
\n
Guideline<\/h2>\n
References<\/h2>\n
\n
\n\n
\n \nParameter<\/th>\n Specification<\/th>\n<\/tr>\n<\/thead>\n \n Appearance<\/td>\n Red, clear liquid<\/td>\n<\/tr>\n \n pH<\/td>\n \u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n \u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n \n Endotoxin<\/td>\n \u00a0NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Suitability<\/td>\n \u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n \n Additive<\/td>\n \u00a0Sodium pyruvate<\/td>\n<\/tr>\n \n Indicator<\/td>\n \u00a0Phenol red<\/td>\n<\/tr>\n \n Mycoplasma Detection<\/td>\n Negative<\/td>\n<\/tr>\n \n Sterility Tested<\/td>\n \u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n \n Form<\/td>\n Liquid<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n \u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n