{"id":59205,"date":"2023-07-21T19:02:09","date_gmt":"2023-07-21T19:02:09","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=59205"},"modified":"2026-07-31T14:56:26","modified_gmt":"2026-07-31T20:56:26","slug":"nuclease-free-water-non-depc","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/nuclease-free-water-non-depc\/","title":{"rendered":"Ultra-Pure Nuclease Free Water (Non-DEPC Treated)"},"content":{"rendered":"<h2>Background<\/h2>\n<p>PurMa\u2122 Ultra-Pure Nuclease Free Water (Non-DEPC Treated) is specialized for highly sophisticated molecular biology studies. Moreover, this water possesses the highest standards for molecular biology studies by removing all contaminants such as:<\/p>\n<ol>\n<li>Organic and non-organic compounds.<\/li>\n<li>Dissolved and particulate matters with any sizes.<\/li>\n<li>Volatile and non-volatile<\/li>\n<li>Reactive, and inert<\/li>\n<li>Hydrophilic and hydrophobic<\/li>\n<li>Dissolved gases.<\/li>\n<\/ol>\n<h2>Special Features<\/h2>\n<ul>\n<li>Purified by Deionization (DI) followed by Reverse Osmosis process (18.2 \u2126M\/cm<sup>2<\/sup>).<\/li>\n<li>Additionally, the trace of heavy metals including Ca<sup>+2<\/sup> and Mg<sup> +2 <\/sup>has been depleted using Ion exchange chromatography resins.<\/li>\n<li>Moreover, it is Double &#8211; Distilled.<\/li>\n<li>Sterilized by 0.1 \u00b5m filters.<\/li>\n<li>And lastly, Packed under N2<\/li>\n<\/ul>\n<p><strong>Important Note:<\/strong> Do NOT expose this water to open air for a long time as it absorbs traces of various ions including bicarbonate.<\/p>\n<p>PurMa\u2122 Non DEPC Treated Ultra-Pure Nuclease Free Water is suitable for high accuracy molecular biology studies, as well as analytical analysis applications such as:<\/p>\n<ol>\n<li>It is definitely the best choice for mRNA &amp; Total RNA Isolation.<\/li>\n<li>Mass spectrometry certainly performs the best with this quality of water.<\/li>\n<li>Electrophoresis, cloning, PCR, RNA, and DNA isolation.<\/li>\n<li>Especially, chromosome analysis or karyotyping as they need high quality water.<\/li>\n<li>Also, northern and southern blotting.<\/li>\n<li>Evaluated for proteases.<\/li>\n<li>Evaluated for phosphatases.<\/li>\n<\/ol>\n<h2>References<\/h2>\n<ol>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31362430\/\" rel=\"nofollow noopener\" target=\"_blank\">A Review on Reverse Osmosis and Nanofiltration Membranes for Water Purification. <\/a><\/span>Yang et al. Polymers (Basel). 2019 Jul 29;11(8):1252. doi: 10.3390\/polym11081252. PMID:<\/li>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35654232\/\" rel=\"nofollow noopener\" target=\"_blank\">Removal of emerging organic micropollutants via modified-reverse osmosis\/nanofiltration membranes: A review. <\/a><\/span>Khoo et al. 2022 Oct;305:135151. 2022.135151. Epub 2022 May 30.<\/li>\n<\/ol>\n<table width=\"460\">\n<tbody>\n<tr>\n<td colspan=\"2\" width=\"460\"><strong>General specifications<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"201\"><strong>Parameter<\/strong><\/td>\n<td width=\"259\"><strong>Specification<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Grade<\/td>\n<td width=\"259\">Ultra-Pure Molecular Biology<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Appearance<\/td>\n<td width=\"259\">Clear<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">pH<\/td>\n<td width=\"259\">5-7<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Osmolality<\/td>\n<td width=\"259\">275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Filtration<\/td>\n<td width=\"259\">Sterile filtered using 0.1 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Indicator<\/td>\n<td width=\"259\">NA<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Conductivity (Measured)<\/td>\n<td width=\"259\">0.056 \u00b5s\/cm<sup>2<\/sup><\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Resistancy (Calculated)<\/td>\n<td width=\"259\">18.2 \u2126m<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Shipping Condition<\/td>\n<td width=\"259\">Ambient<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Refractive index<\/td>\n<td width=\"259\"><em>n<\/em>20\/D 1.34 (lit.)<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" width=\"460\"><strong>Molecular Biology Parameters<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"201\">DNases &amp; RNases<\/td>\n<td width=\"259\">NOT Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">proteases<\/td>\n<td width=\"259\">NOT Detected<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">phosphatases<\/td>\n<td width=\"259\">NOT Detected<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" width=\"460\"><strong>Pathogens Evaluation<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Endotoxin<\/td>\n<td width=\"259\">NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Mycoplasma<\/td>\n<td width=\"259\">Negative<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Mold &amp; Fungi\/Yeast<\/td>\n<td width=\"259\">Negative<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Bacteria<\/td>\n<td width=\"259\">Negative<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Sodium pyruvate<\/td>\n<td width=\"259\">NA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PurMa\u2122 Ultra-Pure Nuclease Free Water (Non-DEPC Treated) is specialized for highly sophisticated molecular biology studies. Moreover, PurMa Biologics manufactures this water by double &#8211; Distillation as well as sterilizing by passing through 0.1\u00b5m filters.<\/p>\n","protected":false},"featured_media":127243,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[78],"product_tag":[617,563,616,615,534],"class_list":["post-59205","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-suited-water-and-water-treatments","product_tag-distillation","product_tag-molecular-biology-water","product_tag-non-depc-treated","product_tag-nuclease-free-water","product_tag-ultra-pure","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/59205","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=59205"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127243"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=59205"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=59205"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=59205"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=59205"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}