{"id":59288,"date":"2023-07-25T17:33:39","date_gmt":"2023-07-25T17:33:39","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=59288"},"modified":"2026-07-31T14:37:09","modified_gmt":"2026-07-31T20:37:09","slug":"water-bath-treatment-500x","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/water-bath-treatment-500x\/","title":{"rendered":"Water Bath Treatment (500X)"},"content":{"rendered":"<h2>Background<\/h2>\n<p>At 37\u00b0C, water creates humidity which makes an excellent environment for growing all types of pathogens. Basically, growing various pathogens in a water bath container in the absence of a preventing agent is inevitable. \u00a0PurMa<sup>TM <\/sup>Water Bath Treatment (500X) prevents growing all the known pathogens in water bath incubators.<\/p>\n<h2>Special Features<\/h2>\n<ol>\n<li>Firstly, dilute PurMa<sup>TM <\/sup>Water Bath Treatment 500 times.<\/li>\n<li>Secondly, PurMa\u2122 Water Bath contains fast-acting biocidal agents with a moderately long duration of action.<\/li>\n<li>PurMa<sup>TM <\/sup>Water Bath Treatment certainly does not cause damage to the stainless steel or copper tray.<\/li>\n<li>Moreover, it is non-toxic, non-volatile.<\/li>\n<li>It actively eliminates bacteria, mold, yeast and most viruses, fungi, and protozoa.<\/li>\n<\/ol>\n<h2>Important Notes<\/h2>\n<ul>\n<li>If the below instruction is followed, PurMa Biologics guarantees NO pathogen growth in your water bath for the duration of 8 weeks.<\/li>\n<li>If you, however, go above 500 times dilution, the treatment will be too weak to be effective.<\/li>\n<li>PurMa\u2122 General Water Bath treatment is not intended for a CO2 incubator.<\/li>\n<\/ul>\n<h2>Instruction to Use<\/h2>\n<ul>\n<li>Primarily, Spray water bath with 70% alcohol before using this product.<\/li>\n<li>Dilute the PurMa<sup>TM <\/sup>Water Bath Treatment 500 times with either molecular biology or cell culture suited water.<\/li>\n<li>Importantly, avoid leaving the water bath lid open overnight, add water if needed.<\/li>\n<\/ul>\n<h2>References:<\/h2>\n<ol>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29847734\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Effects of CO<sub>2<\/sub> water-bath treatment on blood flow and angiogenesis in ischemic hind limb of diabetic rat<\/span>. <\/a>Xu et al. Can J Physiol Pharmacol. 2018 Oct;96(10):1017-1021.. Epub 2018 May 30.<\/li>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23478918\/\" rel=\"nofollow noopener\" target=\"_blank\">Water-bath method for sonographic evaluation of superficial structures of the extremities in children. <\/a><\/span>Krishnamurthy et al. Pediatr Radiol. 2013 Mar;43 Suppl 1:S41-7. Epub 2013 Mar 12.<\/li>\n<\/ol>\n<table width=\"460\">\n<tbody>\n<tr>\n<td width=\"201\"><strong>Parameter<\/strong><\/td>\n<td width=\"259\"><strong>Specification<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Appearance<\/td>\n<td width=\"259\">Red liquid<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">pH<\/td>\n<td width=\"259\">7.4 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Osmolality<\/td>\n<td width=\"259\">275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Endotoxin<\/td>\n<td width=\"259\">NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Mycoplasma<\/td>\n<td width=\"259\">Negative<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Suitability<\/td>\n<td width=\"259\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Indicator<\/td>\n<td width=\"259\">NA<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Sterility Tested<\/td>\n<td width=\"259\">Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Form<\/td>\n<td width=\"259\">Liquid<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Shipping Condition<\/td>\n<td width=\"259\">Dry Ice<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>At 37\u00b0C, water creates humidity which makes an excellent environment for growing all types of pathogens. Basically, growing various pathogens in a water bath container in the absence of a prevention agent is inevitable. PurMa<sup>TM <\/sup>Water Bath Treatment (500X) prevents growing all the known pathogens in water bath incubators.<\/p>\n","protected":false},"featured_media":127242,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[78],"product_tag":[565,564],"class_list":["post-59288","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-suited-water-and-water-treatments","product_tag-preventing-pathogens","product_tag-waterbath-treatment","entry","has-media","first","instock","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/59288","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=59288"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127242"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=59288"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=59288"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=59288"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=59288"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}